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Abstract:

We have previously reported that in the distal stump of ligated sciatic nerves, there is a change in the distribution of myelin basic protein (MBP) and P 0 protein immunoreactivities. These results agreed with the studies of myelin isolated from the distal stump of animals submitted to ligation of the sciatic nerve, showing a gradual increase in a 14 kDa band with an electrophoretic mobility similar to that of an MBP isoform, among other changes. This band, which was resolved into two bands of 14 and 15 kDa using a 16% gel, was found to contain a mixture of MBP fragments and peptides with great homology with α- and β-globins. In agreement with these results, we have demonstrated that the mRNA of α-globin is present in the proximal and distal stumps of the ligated nerve. It is also detected at very low levels in Schwann cells isolated from normal nerves. These results could be due to the presence of α- and/or β-globin arising from immature cells of the erythroid series. Also, they could be present in macrophages, which spontaneously migrate to the injured nerve to promote the degradation of myelin proteins. Cells isolated from normal adult rat bone marrow which were injected intraortically were found to migrate to the injured area. These cells could contribute to the remyelination of the damaged area participating in the removal of myelin debris, through their transdifferentiation into Schwann cells or through their fusion with preexisting Schwann cells in the distal stump of the injured sciatic nerve. © 2006 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
Autor:Setton-Avruj, C.P.; Musolino, P.L.; Salis, C.; Alló, M.; Bizzozero, O.; Villar, M.J.; Soto, E.F.; Pasquini, J.M.
Filiación:Department of Biological Chemistry, Institute of Biological and Physical Chemistry (IQUIFIB), School of Pharmacy and Biochemistry, 956 Buenos Aires, Argentina
School of Biomedical Sciences, Austral University, Av. Pte. Peron 1500, B1629AHJ Pilar, Prov. Buenos Aires, Argentina
Department of Cell Biology and Physiology, University of New Mexico, Health Sciences Center, Albuquerque, NM 87131, United States
Laboratory of Physiology and Molecular Biology (LFBM), Department of Physiology and Molecular and Cell Biology, IFIBYNE, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Palabras clave:α-Globin; Bone marrow mononuclear cells; Macrophages; Myelin proteins; Peripheral myelin; Wallerian degeneration; alpha globin; beta globin; glycine dehydrogenase (decarboxylating); isoprotein; messenger RNA; myelin; myelin basic protein; peptide; animal cell; animal experiment; animal model; animal tissue; article; bone marrow cell; cell isolation; cell migration; controlled study; electrophoretic mobility; erythroid cell; immunoreactivity; macrophage; nerve cell differentiation; nerve degeneration; nerve injury; nerve ligation; nerve regeneration; nonhuman; polyacrylamide gel electrophoresis; priority journal; protein degradation; protein isolation; rat; real time polymerase chain reaction; Schwann cell; sciatic nerve; Western blotting; Animals; Blotting, Western; Bone Marrow Cells; Cell Movement; Electrophoresis, Polyacrylamide Gel; Female; Globins; Immunohistochemistry; Male; Myelin Basic Proteins; Nerve Degeneration; Nerve Regeneration; Nuclease Protection Assays; Peptides; Peripheral Nerves; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Schwann Cells; Sciatic Nerve; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin
Año:2007
Volumen:203
Número:2
Página de inicio:568
Página de fin:578
DOI: http://dx.doi.org/10.1016/j.expneurol.2006.09.024
Título revista:Experimental Neurology
Título revista abreviado:Exp. Neurol.
ISSN:00144886
CODEN:EXNEA
CAS:glycine dehydrogenase (decarboxylating), 37259-67-9; Globins, 9004-22-2; Myelin Basic Proteins; Peptides; RNA, Messenger; Trypsin, EC 3.4.21.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v203_n2_p568_SettonAvruj

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Citas:

---------- APA ----------
Setton-Avruj, C.P., Musolino, P.L., Salis, C., Alló, M., Bizzozero, O., Villar, M.J., Soto, E.F.,..., Pasquini, J.M. (2007) . Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process. Experimental Neurology, 203(2), 568-578.
http://dx.doi.org/10.1016/j.expneurol.2006.09.024
---------- CHICAGO ----------
Setton-Avruj, C.P., Musolino, P.L., Salis, C., Alló, M., Bizzozero, O., Villar, M.J., et al. "Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process" . Experimental Neurology 203, no. 2 (2007) : 568-578.
http://dx.doi.org/10.1016/j.expneurol.2006.09.024
---------- MLA ----------
Setton-Avruj, C.P., Musolino, P.L., Salis, C., Alló, M., Bizzozero, O., Villar, M.J., et al. "Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process" . Experimental Neurology, vol. 203, no. 2, 2007, pp. 568-578.
http://dx.doi.org/10.1016/j.expneurol.2006.09.024
---------- VANCOUVER ----------
Setton-Avruj, C.P., Musolino, P.L., Salis, C., Alló, M., Bizzozero, O., Villar, M.J., et al. Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process. Exp. Neurol. 2007;203(2):568-578.
http://dx.doi.org/10.1016/j.expneurol.2006.09.024