Artículo

Adamo, A.M.; Paez, P.M.; Escobar Cabrera, O.E.; Wolfson, M.; Franco, P.G.; Pasquini, J.M.; Soto, E.F. "Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin" (2006) Experimental Neurology. 198(2):519-529
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Abstract:

Twenty-one-day-old Wistar rats were fed a diet containing 0.6% cuprizone for 2 weeks. Studies carried out after withdrawal of cuprizone showed histological evidences of marked demyelination in the corpus callosum. Biochemical studies of isolated myelin showed a marked decrease in myelin proteins, phospholipids, and galactocerebrosides as well as a marked decrease in myelin yield. Treatment of these animals with a single intracranial injection of 350 ng of apotransferrin at the time of withdrawal of cuprizone induced a marked increase in myelin deposition resulting in a significantly improved remyelination, evaluated by histological, immunocytochemical, and biochemical parameters, in comparison to what was observed in spontaneous recovery. Immunocytochemical studies of cryotome sections to analyze developmental parameters of the oligodendroglial cell population at the time of termination of cuprizone and at different times thereafter showed that in the untreated animals, there was a marked increase in the number of NG2-BrdU-positive precursor cells together with a marked decrease in MBP expression at the peak of cuprizone-induced demyelination. As expected, the amount of precursor cells decreased markedly during spontaneous remyelination and was accompanied by an increase in MBP reactivity. In the apotransferrin-treated animals, these phenomena occurred much faster, and remyelination was much more efficient than in the untreated controls. The results of this study suggest that apotransferrin is a very active promyelinating agent which could be important for the treatment of certain demyelinating conditions. © 2006 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin
Autor:Adamo, A.M.; Paez, P.M.; Escobar Cabrera, O.E.; Wolfson, M.; Franco, P.G.; Pasquini, J.M.; Soto, E.F.
Filiación:Instituto de Química y Físicoquímica Biológica (IQUIFIB), UBA-CONICET, Departamento de Química Biológica, Buenos Aires, C1113AAD, Argentina
Palabras clave:Apotransferrin; Cuprizone; Demyelinating diseases; Demyelination; Oligodendroglial cell differentiation; Remyelination; apotransferrin; broxuridine; cuprizone; galactosylceramide; myelin protein; phospholipid; animal experiment; animal model; animal tissue; article; chemical analysis; controlled study; corpus callosum; demyelinating disease; demyelination; female; histology; immunocytochemistry; male; nonhuman; priority journal; protein expression; protein isolation; rat; remyelinization; statistical significance; stem cell; Analysis of Variance; Animals; Animals, Newborn; Antigens; Antigens, CD11b; Apoproteins; Brain; Bromodeoxyuridine; Cell Count; Cuprizone; Cytoskeletal Proteins; Demyelinating Diseases; Drug Interactions; Galactolipids; Glial Fibrillary Acidic Protein; Immunohistochemistry; Indoles; Myelin Basic Proteins; Myelin Sheath; Proteoglycans; Rats; Rats, Wistar; Recovery of Function; Regeneration; Time Factors; Transferrin
Año:2006
Volumen:198
Número:2
Página de inicio:519
Página de fin:529
DOI: http://dx.doi.org/10.1016/j.expneurol.2005.12.027
Título revista:Experimental Neurology
Título revista abreviado:Exp. Neurol.
ISSN:00144886
CODEN:EXNEA
CAS:broxuridine, 59-14-3; cuprizone, 370-81-0; galactosylceramide, 85305-88-0; Antigens; Antigens, CD11b; Apoproteins; apotransferrin; Bromodeoxyuridine, 59-14-3; chondroitin sulfate proteoglycan 4; Cuprizone, 370-81-0; Cytoskeletal Proteins; DAPI, 47165-04-8; Galactolipids; Glial Fibrillary Acidic Protein; Indoles; Myelin Basic Proteins; Proteoglycans; Transferrin, 11096-37-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v198_n2_p519_Adamo

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Citas:

---------- APA ----------
Adamo, A.M., Paez, P.M., Escobar Cabrera, O.E., Wolfson, M., Franco, P.G., Pasquini, J.M. & Soto, E.F. (2006) . Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin. Experimental Neurology, 198(2), 519-529.
http://dx.doi.org/10.1016/j.expneurol.2005.12.027
---------- CHICAGO ----------
Adamo, A.M., Paez, P.M., Escobar Cabrera, O.E., Wolfson, M., Franco, P.G., Pasquini, J.M., et al. "Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin" . Experimental Neurology 198, no. 2 (2006) : 519-529.
http://dx.doi.org/10.1016/j.expneurol.2005.12.027
---------- MLA ----------
Adamo, A.M., Paez, P.M., Escobar Cabrera, O.E., Wolfson, M., Franco, P.G., Pasquini, J.M., et al. "Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin" . Experimental Neurology, vol. 198, no. 2, 2006, pp. 519-529.
http://dx.doi.org/10.1016/j.expneurol.2005.12.027
---------- VANCOUVER ----------
Adamo, A.M., Paez, P.M., Escobar Cabrera, O.E., Wolfson, M., Franco, P.G., Pasquini, J.M., et al. Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin. Exp. Neurol. 2006;198(2):519-529.
http://dx.doi.org/10.1016/j.expneurol.2005.12.027