Navegar

Colección

Datos Estadísticas

Artículo

Gonzalez Deniselle, M.C.; Garay, L.; Gonzalez, S.; Guennoun, R.; Schumacher, M.; De Nicola, A.F. "Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease" (2005) Experimental Neurology. 195(2):518-523
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The Wobbler mouse, a mutant characterized by motoneuron degeneration in the cervical spinal cord, has been used to test the efficacy of novel treatments for human motoneuron diseases (HMD). Previous reports have shown that slow axonal transport is impaired in Wobblers and other models of HMD. Since progesterone (PROG) corrects some morphological, molecular, and functional abnormalities of Wobbler mice, we studied if steroid exposure for 8 weeks restored retrograde axonal transport by measuring motoneuron labeling after injection of fluorogold into the limb muscles. The dye was injected into forelimb biceps bracchii and flexor or into the rearlimb gastrocnemius muscles; 6 days later, the number of fluorescent motoneurons and the total number of cresyl violet stained motoneurons were counted in the cervical (C5-T1) or lumbar (L3-L5) spinal cord regions. A pronounced reduction (- 42.2%) of the percent of fluorescent motoneurons in Wobbler mice cervical cord was noted, which was significantly corrected after PROG treatment. In contrast, labeling of lumbar motoneurons was not reduced in Wobbler mice and was not affected by PROG treatment. In no case PROG showed an effect in control mice. Concomitantly, PROG slightly but significantly increased biceps weight of Wobbler mice. Behaviorally, PROG-treated Wobblers performed better on a motor test (hanging time from a horizontal rope) compared to untreated counterparts. We postulate a dual role for PROG in the Wobbler mouse, in part by prevention of motoneuron degeneration and also by enhancement of axonal transport. The latter mechanism could improve the traffic of neurotrophic factors from the forelimb muscles into the ailing motoneurons, improving neuromuscular function in this murine model of HMD. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease
Autor:Gonzalez Deniselle, M.C.; Garay, L.; Gonzalez, S.; Guennoun, R.; Schumacher, M.; De Nicola, A.F.
Filiación:Laboratory of Neuroendocrine Biochemistry, Instituto de Biología Y Medicina Experimental, University of Buenos, Obligado 2490, 1428 Buenos Aires, Argentina
Department of Biochemistry, Faculty of Medicine, University of Buenos, Obligado 2490, 1428 Buenos Aires, Argentina
INSERM U488, University Paris 11, Kremlin-Bicêtre, France
Instituto Universitario de Ciencias de la Salud, Fundación H. A. Barceló, Buenos Aires, Argentina
Instituto de Biologia Y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Axonal transport; Fluorogold; Motoneuron degeneration; Progesterone; Spinal cord; Wobbler mouse; fluorescent dye; fluorogold; neurotrophic factor; progesterone; animal behavior; animal experiment; animal model; animal tissue; arm muscle; article; biceps brachii muscle; cell count; cell labeling; cervical spinal cord; controlled study; drug effect; drug exposure; drug mechanism; female; flexor muscle; fluorescence; gastrocnemius muscle; leg muscle; male; motoneuron; motor neuron disease; motor performance; mouse; mouse mutant; nerve degeneration; nerve fiber transport; neuromuscular function; nonhuman; priority journal; spinal cord motoneuron; weight gain; Animals; Axonal Transport; Behavior, Animal; Cervical Vertebrae; Disease Models, Animal; Female; Male; Mice; Mice, Neurologic Mutants; Motor Neuron Disease; Motor Neurons; Progestins; Spinal Cord; Stilbamidines; Upper Extremity
Año:2005
Volumen:195
Número:2
Página de inicio:518
Página de fin:523
DOI: http://dx.doi.org/10.1016/j.expneurol.2005.06.015
Título revista:Experimental Neurology
Título revista abreviado:Exp. Neurol.
ISSN:00144886
CODEN:EXNEA
CAS:fluorogold, 82785-14-6; progesterone, 57-83-0; 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; Progestins; Stilbamidines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v195_n2_p518_GonzalezDeniselle

Referencias:

  • Abe, K., Morita, S., Kikuchi, T., Itoyama, S., Protective effect of a novel free-radical scavenger, OPC-14117, on wobbler mouse motor neuron disease (1997) J. Neurosci. Res., 48, pp. 63-70
  • Azcoitia, I., Leonelli, E., Magnaghi, V., Veiga, S., Garcia-Segura, L.M., Melcangi, R.V., Progesterone and its derivatives dihydroprogesterone and tetrahydroprogesterone reduce myelin fiber morphological abnormalities and myelin fiber loss in the sciatic nerve of aged rats (2003) Neurobiol. Aging, 24, pp. 853-860
  • Boillée, S.M., Peschanski, M., Junier, M.-P., The Wobbler mouse: A neurodegeneration jigsaw puzzle (2003) Mol. Neurobiol., 28, pp. 65-106
  • Bose, P., Fielding, R., Vacca-Galloway, L.L., Effects of assisted feeding on Wobbler mouse motoneuron disease and on serotonergic and peptidergic sprouting in the cervical spinal ventral horn (1999) Brain Res. Bull., 48, pp. 429-439
  • Clarke, P.G.H., Developmental cell death: Morphological diversity and multiple mechanisms (1990) Anat. Embryol., 181, pp. 195-213
  • Cudkowicz, M.E., Brown, R.H., Amyotrophic lateral sclerosis and related motor neuron diseases (1998) Principles of Molecular Medicine, pp. 907-911. , J.L. Jameson Humana Press New Jersey
  • Dave, K.R., Bradley, W.G., Perez-Pinzón, M.A., Early mitochondrial dysfunction occurs in motor cortex and spinal cord at the onset of disease in the Wobbler mouse (2003) Exp. Neurol., 182, pp. 412-420
  • Desarnaud, F., Do Thi, A.Q.M., Brown, G., Lemke, G., Suter, U., Baulieu, E.E., Schumacher, M., Progesterone stimulates the activity of the promoters of peripheral myelin protein-22 and protein zero genes in Schwann cells (1998) J. Neurochem., 71, pp. 1765-1768
  • Duchen, L.W., Strick, S.J., An hereditary motor neurone disease with progressive denervation of muscle in the mouse: The mutant "wobbler" (1968) J. Neurol., Neurosurg. Psychiatry, 31, pp. 535-542
  • González, S.L., Labombarda, F., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F., Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord (2004) Neuroscience, 125, pp. 605-614
  • González Deniselle, M.C., González, S., Lima, A., Wilkin, G., De Nicola, A.F., The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPHDiaphorase in the spinal cord of Wobbler mouse, a model for amyotrophic lateral sclerosis (1999) Neurochem. Res., 24, pp. 1-8
  • González Deniselle, M.C., López Costa, J.J., Pecci Saavedra, J., Pietranera, L., González, S.L., Garay, L., Guennoun, R., De Nicola, A.F., Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease (2002) Neurobiol. Dis., 11, pp. 457-468
  • González Deniselle, M.C., López Costa, J.J., González, S.L., Labombarda, F., Garay, L., Guennoun, R., Schumacher, M., De Nicola, A.F., Basis of progesterone in spinal cord neurodegeneration (2003) J. Steroid Biochem. Mol. Biol., 83, pp. 199-209
  • González Deniselle, M.C., Garay, L., López-Costa, J.J., González, S., Mougel, A., Guennoun, R., Schumacher, M., De Nicola, A.F., Progesterone treatment reduces NADPH-Diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease (2004) Brain Res., 1014, pp. 71-79
  • Haenggeli, C., Kato, A.C., Rapid and reproducible methods using fluorogold for labelling a subpopulation of cervical motoneurons: An application in the wobbler mouse (2002) J. Neurosci. Methods, 116, pp. 119-124
  • Ikeda, K., Iwasaki, Y., Kinoshita, M., Neuronal nitric oxide synthase inhibitor, 7-nitroindazole, delays motor dysfunction and spinal motoneuron degeneration in the wobbler mouse (1998) J. Neurol. Sci., 160, pp. 9-15
  • Ishiyama, T., Okada, R., Nishibe, H., Mitsumoto, H., Nakayama, C., Riluzole slows the progression of neuromuscular dysfunction in the wobbler mouse motor neuron disease (2004) Brain Res., 1019, pp. 226-236
  • Junier, M.-P., Coulpier, M.N., Le Forestier, N., Cadusseau, J., Suzuki, F., Peschanski, M., Dreyfus, P.A., Transforming growth factor alpha (TGF alpha) expression in degenerating motoneurons of the murine mutant Wobbler: A neuronal signal for astrogliosis? (1994) J. Neurosci., 14, pp. 4206-4216
  • Junier, M.P., Legendre, P., Esguerra, C.V., Tinel, M., Coulpier, M., Dreyfus, P.A., Bahr, M., Regulation of growth factor gene expression in degenerating motoneurons of the murine mutant wobbler: A cellular patch-sampling/RT-PCR study (1998) Mol. Cell. Neurosci., 12, pp. 168-177
  • Kaupmann, K., Simon-Chazottes, D., Guener, J.L., Jokusch, J., Wobbler, a mutation affecting motoneuron survival and gonadal functions in the mouse, maps to proximal chromosome 11 (1992) Genomics, 13, pp. 39-43
  • Labombarda, F., González, S.L., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F., Cellular basis for progesterone neuroprotection in the injured spinal cord (2002) J. Neurotrauma, 19, pp. 343-355
  • Leong, S.K., Ling, E.A., Labelling neurons with fluorescent dyes administered via intravenous, subcutaneous or intraperitoneal route (1990) J. Neurosci. Methods, 32, pp. 15-23
  • Melcangi, R.C., Magnaghi, V., Martini, L., Aging in peripheral nerves: Regulation of myelin protein genes by steroid hormones (2000) Prog. Neurobiol., 60, pp. 291-308
  • Mitsumoto, H., Bradley, W.G., Murine motor neuron disease (the Wobbler mouse): Degeneration and regeneration of the lower motor neuron (1982) Brain Res., 105, pp. 811-834
  • Mitsumoto, H., Gambetti, P., Impaired axonal transport in wobbler mouse motor neuron disease (1986) Ann. Neurol., 19, pp. 36-43
  • Mohajeri, M.H., Figlewicz, D.A., Bohn, M.C., Selective loss of α motoneurons innervating the medial gastrocnemius muscle in a mouse model of amyotrophic lateral sclerosis (1998) Exp. Neurol., 150, pp. 329-336
  • Pernas Alonso, R., Perrone-Capano, C.F., Volpicelli, F., Di Porzio, H., Regionalized neurofilament accumulation and motoneuron degeneration are linked phenotypes in Wobbler neuromuscular disease (2001) Neurobiol. Dis., 8, pp. 581-589
  • Popper, P., Farber, D.B., Micevych, P.E., Minoofar, K., Bronstein, J.M., RTPM-2 expression and TUNEL staining in neurodegenerative diseases: Studies in wobbler and rd mice (1997) Exp. Neurol., 143, pp. 246-254
  • Price, D.L., Cleveland, D.W., Koliatsis, V.E., Motor neuron disease and animal models (1994) Neurobiol. Dis., 1, pp. 3-11
  • Schmued, L.C., Fallon, J.H., Fluoro-Gold: A new fluorescent retrograde axonal tracer with numerous unique properties (1986) Brain Res., 377, pp. 147-154
  • Tsuzaka, K.T., Ishiyama, T., Pioro, E.P., Mitsumoto, H., Role of brain-derived neurotrophic factor in Wobbler mouse motor neuron disease (2001) Muscle Nerve, 24, pp. 474-480
  • Yung, K.K.L., Tang, F., Vacca-Galloway, L.L., Changes of neuropeptides in spinal cord and brain stem of Wobbler mouse at different stages of motoneuron disease (1992) Neuroscience, 50, pp. 209-222

Citas:

---------- APA ----------
Gonzalez Deniselle, M.C., Garay, L., Gonzalez, S., Guennoun, R., Schumacher, M. & De Nicola, A.F. (2005) . Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease. Experimental Neurology, 195(2), 518-523.
http://dx.doi.org/10.1016/j.expneurol.2005.06.015
---------- CHICAGO ----------
Gonzalez Deniselle, M.C., Garay, L., Gonzalez, S., Guennoun, R., Schumacher, M., De Nicola, A.F. "Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease" . Experimental Neurology 195, no. 2 (2005) : 518-523.
http://dx.doi.org/10.1016/j.expneurol.2005.06.015
---------- MLA ----------
Gonzalez Deniselle, M.C., Garay, L., Gonzalez, S., Guennoun, R., Schumacher, M., De Nicola, A.F. "Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease" . Experimental Neurology, vol. 195, no. 2, 2005, pp. 518-523.
http://dx.doi.org/10.1016/j.expneurol.2005.06.015
---------- VANCOUVER ----------
Gonzalez Deniselle, M.C., Garay, L., Gonzalez, S., Guennoun, R., Schumacher, M., De Nicola, A.F. Progesterone restores retrograde labeling of cervical motoneurons in Wobbler mouse motoneuron disease. Exp. Neurol. 2005;195(2):518-523.
http://dx.doi.org/10.1016/j.expneurol.2005.06.015