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Abstract:

A proliferation assay based on the production of mini-units of tissue was adopted and modified for the simultaneous determination of cell proliferation rate and the effect of genistein in rat cerebral cortex. Mini- units of tissue were produced from rat cerebral cortex immediately after killing the animal and incubated with culture medium containing 3H-methyl- thymidine during 90 min. The proliferation rate was assessed by measurement of 3H-methyl-thymidine incorporation into trichloroacetic acid insoluble material/mg of protein/min. The mini-unit method preserves the neural-cell topological relation existing in vivo and, in addition, has several additional advantages: (1) the short incubation time required limits the metabolic changes, (2) the sensitivity to drugs can be assessed simultaneously with the cell proliferation rate, (3) the complete procedure can be performed within 4-6 h, and (4) many experiments can be performed with the tissue from one animal. Genistein in doses from 10 to 100 μM inhibited cell proliferation in a concentration-dependent manner. The percentage of inhibition was highest in young animals and decreased with increasing age. This method is a powerful tool for the study of drugs with short-time onset mechanisms of action and can be useful for the screening of new drugs.

Registro:

Documento: Artículo
Título:Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex
Autor:Yakisich, J.S.; Sidén, A.; Idoyaga Vargas, V.; Eneroth, P.; Cruz, M.
Filiación:Applied Biochemistry, Clinical Research Center, Huddinge University Hospital, Huddinge, Sweden
Department of Neurology, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden
Fac. de Ciencias Exactas y Naturales, Inst. de Invest. Bioquimicas F., Buenos Aires, Argentina
Palabras clave:Cell proliferation; Cerebral cortex; DNA synthesis; Drug sensitivity; Genistein; Tyrosine kinase inhibitor; genistein; protein tyrosine kinase; animal cell; animal tissue; article; brain cortex; cell metabolism; cell proliferation; culture medium; DNA replication; DNA synthesis; DNA synthesis inhibition; drug sensitivity; nonhuman; priority journal; rat
Año:1998
Volumen:151
Número:2
Página de inicio:194
Página de fin:202
DOI: http://dx.doi.org/10.1006/exnr.1998.6814
Título revista:Experimental Neurology
Título revista abreviado:Exp. Neurol.
ISSN:00144886
CODEN:EXNEA
CAS:genistein, 446-72-0; protein tyrosine kinase, 80449-02-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v151_n2_p194_Yakisich

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Citas:

---------- APA ----------
Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., Eneroth, P. & Cruz, M. (1998) . Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex. Experimental Neurology, 151(2), 194-202.
http://dx.doi.org/10.1006/exnr.1998.6814
---------- CHICAGO ----------
Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., Eneroth, P., Cruz, M. "Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex" . Experimental Neurology 151, no. 2 (1998) : 194-202.
http://dx.doi.org/10.1006/exnr.1998.6814
---------- MLA ----------
Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., Eneroth, P., Cruz, M. "Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex" . Experimental Neurology, vol. 151, no. 2, 1998, pp. 194-202.
http://dx.doi.org/10.1006/exnr.1998.6814
---------- VANCOUVER ----------
Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., Eneroth, P., Cruz, M. Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex. Exp. Neurol. 1998;151(2):194-202.
http://dx.doi.org/10.1006/exnr.1998.6814