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Abstract:

Ocular herpes simplex virus type-1 (HSV-1) infections remain an important cause of corneal disease which may result in a loss of vision. Meliacine (MA), an antiviral activity present in crude leaf extracts of Melia azedarach L. that inhibits HSV-1 multiplication in vitro, was studied in a murine herpetic stromal keratitis experimental model. Adult Balb/c mice were inoculated with HSV-1 at their corneas after abrasion. MA was administered topically three times a day for 3 consecutive days, beginning at 24 and 96 hr after infection. Infected animals treated or not with MA were monitored for the development of ocular disease by a binocular microscope for 16 days. MA significantly reduced the incidence and the severity of blepharitis, neovascularization and stromal keratitis with respect to untreated infected mice, regardless the schedule of treatment assayed. Histological examination of corneas from MA-treated animals revealed no tissue damage, whereas corneal samples from untreated infected mice showed inflammation, vascularization and necrosis. In uninfected mice treated with MA, we found no evidence of corneal damage and histopathological studies showed no changes in the corneas of these mice. Treatment with MA at 24 hours post-infection (h.p.i.) reduced viral multiplication in the eye by 1-1.5 orders of magnitude. Studies on latency revealed that MA sligthly affected the establishment of a latent infection. Thus, MA proved to exert an antiviral action on the development of herpetic stromal keratitis when supplied by post-treatment. Unexpectedly, treatment with MA after 96 h.p.i prevented ocular disease, suggesting an in vivo immunomodulating activity of MA. © 2002 Elsevier Science Ltd.

Registro:

Documento: Artículo
Título:Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
Autor:Paula Pifarré, M.; Berra, A.; Coto, C.E.; Alché, L.E.
Filiación:Dept. of Biochem., Sch. of Sci., Univ. of Buenos Aires, Pabellon II, Buenos Aires 1428, Argentina
Laboratory of Ocular Immunology, School of Medicine, University of Buenos Aires, J.E. Uriburu 950, Buenos Aires, Argentina
Palabras clave:Herpes simplex virus; In vivo; Latency; Melia azedarach L.; Murine model; Plant antiviral; Stromal keratitis; Melia azedarach extract; meliacine; plant extract; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antiviral activity; article; controlled study; disease severity; Herpes simplex virus 1; histopathology; immunomodulation; inoculation; keratitis; microscopy; mouse; necrosis; nonhuman; priority journal; vascularization
Año:2002
Volumen:75
Número:3
Página de inicio:327
Página de fin:334
DOI: http://dx.doi.org/10.1016/S0014-4835(02)92027-0
Título revista:Experimental Eye Research
Título revista abreviado:Exp. Eye Res.
ISSN:00144835
CODEN:EXERA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144835_v75_n3_p327_PaulaPifarre

Referencias:

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Citas:

---------- APA ----------
Paula Pifarré, M., Berra, A., Coto, C.E. & Alché, L.E. (2002) . Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice. Experimental Eye Research, 75(3), 327-334.
http://dx.doi.org/10.1016/S0014-4835(02)92027-0
---------- CHICAGO ----------
Paula Pifarré, M., Berra, A., Coto, C.E., Alché, L.E. "Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice" . Experimental Eye Research 75, no. 3 (2002) : 327-334.
http://dx.doi.org/10.1016/S0014-4835(02)92027-0
---------- MLA ----------
Paula Pifarré, M., Berra, A., Coto, C.E., Alché, L.E. "Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice" . Experimental Eye Research, vol. 75, no. 3, 2002, pp. 327-334.
http://dx.doi.org/10.1016/S0014-4835(02)92027-0
---------- VANCOUVER ----------
Paula Pifarré, M., Berra, A., Coto, C.E., Alché, L.E. Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice. Exp. Eye Res. 2002;75(3):327-334.
http://dx.doi.org/10.1016/S0014-4835(02)92027-0