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Abstract:

The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974.

Registro:

Documento: Artículo
Título:Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
Autor:Medrano, E.; Piras, R.; Mordoh, J.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina
Palabras clave:colchicine; cytochalasin b; phytohemagglutinin; sepharose; vinblastine; dna synthesis; human; in vitro study; lymphocyte; lymphocyte membrane; lymphocyte transformation; normal human; theoretical study; thymidine h 3; Binding Sites; Cell Division; Cell Survival; Colchicine; Cytochalasin B; DNA; Human; In Vitro; Lectins; Lymphocyte Activation; Lymphocytes; Thymidine; Time Factors; Tritium; Vinblastine
Año:1974
Volumen:86
Número:2
Página de inicio:295
Página de fin:300
DOI: http://dx.doi.org/10.1016/0014-4827(74)90716-2
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:colchicine, 64-86-8; cytochalasin B, 14930-96-2; phytohemagglutinin, 9008-97-3; sepharose, 61970-08-9; vinblastine, 865-21-4; Colchicine, 64-86-8; Cytochalasin B, 14930-96-2; DNA, 9007-49-2; Lectins; Thymidine, 50-89-5; Tritium, 10028-17-8; Vinblastine, 865-21-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v86_n2_p295_Medrano

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Citas:

---------- APA ----------
Medrano, E., Piras, R. & Mordoh, J. (1974) . Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin. Experimental Cell Research, 86(2), 295-300.
http://dx.doi.org/10.1016/0014-4827(74)90716-2
---------- CHICAGO ----------
Medrano, E., Piras, R., Mordoh, J. "Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin" . Experimental Cell Research 86, no. 2 (1974) : 295-300.
http://dx.doi.org/10.1016/0014-4827(74)90716-2
---------- MLA ----------
Medrano, E., Piras, R., Mordoh, J. "Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin" . Experimental Cell Research, vol. 86, no. 2, 1974, pp. 295-300.
http://dx.doi.org/10.1016/0014-4827(74)90716-2
---------- VANCOUVER ----------
Medrano, E., Piras, R., Mordoh, J. Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin. Exp. Cell Res. 1974;86(2):295-300.
http://dx.doi.org/10.1016/0014-4827(74)90716-2