Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The growth factor erythropoietin (Epo) has shown neuronal protective action in addition to its well known proerythroid activity. Furthermore, Epo has dealt with cellular inflammation by inhibiting the expression of several proinflammatory cytokines, such as IL-1 and TNF-α. The action of TNF can have both apoptotic and antiapoptotic consequences due to altered balance between different cell signalling pathways. This work has focused on the apoptotic effects of this cytokine and the potential protective action of Epo. The model we used was neuroblastoma SH-SY5Y cells cultured in the presence of 25 ng/ml TNF-α or pretreated with 25 U/ml Epo for 12 h before the addition of TNF-α. Apoptosis was evaluated by differential cell count after Hoechst staining, analysis of DNA ladder pattern, and measurement of caspase activity. Despite its ability to induce NF-κB nuclear translocation, TNF-α induced cell death, which was found to be associated to upregulation of TNF Receptor 1 expression. On the other hand, cells activated by Epo became resistant to cell death. Prevention of death receptor upregulation and caspase activation may explain this antiapoptotic effect of Epo, which may be also favoured by the induction of a higher expression of protective factors, such as Bcl-2 and NF-κB, through mechanisms involving Jak/STAT and PI3K signalling pathways. © 2008 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells
Autor:Pregi, N.; Wenker, S.; Vittori, D.; Leirós, C.P.; Nesse, A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, Piso 4, Ciudad Universitaria, C1428EHA Ciudad Autonoma de Buenos Aires, Argentina
Palabras clave:Apoptosis; Erythropoietin; Inflammation; Neuroprotection; NF-κB; SH-SY5Y cells; TNF-α; caspase; erythropoietin; Janus kinase; phosphatidylinositol 3 kinase; STAT protein; tumor necrosis factor alpha; tumor necrosis factor receptor 1; apoptosis; article; cell death; cell line; controlled study; enzyme activation; enzyme activity; human; human cell; neuroprotection; priority journal; protein transport
Año:2009
Volumen:315
Número:3
Página de inicio:419
Página de fin:431
DOI: http://dx.doi.org/10.1016/j.yexcr.2008.11.005
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:caspase, 186322-81-6; erythropoietin, 11096-26-7; Janus kinase, 161384-16-3; phosphatidylinositol 3 kinase, 115926-52-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v315_n3_p419_Pregi

Referencias:

  • Ghezzi, P., Brines, M., Erythropoietin as an antiapoptotic, tissue-protective cytokine (2004) Cell Death Differ., 11 (SUPPL. 1), pp. S37-S44
  • Ehrenreich, H., Aust, C., Krampe, H., Jahn, H., Jacob, S., Herrmann, M., Sirén, A.-L., Erythropoietin: novel approaches to neuroprotection in human brain disease (2004) Metab. Brain Dis., 19, pp. 195-206
  • Um, M., Lodish, H.F., Antiapoptotic effects of erythropoietin in differentiated neuroblastoma SH-SY5Y cells require activation of both the STAT5 and AKT signalling pathways (2006) J. Biol. Chem., 281, pp. 5648-5656
  • Chen, Z.-Y., Asavaritikrai, P., Prchal, J.T., Noguchi, C.T., Endogenous erythropoietin signaling is required for normal neural progenitor cell proliferation (2007) J. Biol. Chem., 282, pp. 25875-25883
  • Silva, M., Grillot, D., Benito, A., Richard, C., Nunez, G., Fernandez-Luna, J.L., Erythropoietin can promote erythroid progenitor survival by repressing apoptosis through Bcl-XL and Bcl-2 (1996) Blood, 88, pp. 1576-1582
  • Pregi, N., Vittori, D., Perez, G., Leirós, C.P., Nesse, A., Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells (2006) Biochim. Biophys. Acta, 1763, pp. 238-246
  • Kretz, A., Happold, C.J., Marticke, J.K., Isenmann, S., Erythropoietin promotes regeneration of adult CNS neurons via Jak2/AKT pathway activation (2005) Mol. Cell. Neurosci., 29, pp. 569-579
  • Chen, G., Shi, J.X., Hang, C.H., Xie, W., Liu, J., Liu, X., Inhibitory effects on cerebral inflammatory agents that accompany traumatic brain injury in a rat model: a potential neuroprotective mechanism of recombinant human erythropoietin (RHUEPO) (2007) Neurosci. Lett., 425, pp. 177-182
  • Aggarwal, B.B., Signalling pathways of the TNF superfamily: a double-edged sword (2003) Nat. Rev. Immunol., 3, pp. 745-756
  • Hehlgans, T., Pfeffer, K., The intriguing biology of the tumor necrosis factor/tumor necrosis factor receptor superfamily: players, rules and the games (2005) Immunology, 115, pp. 1-20
  • Tucker, S.J., Rae, C., Littlejon, A.F., Paul, A., MacEwan, D.J., Switching leukemia cell phenotype between life and death (2004) Proc. Natl. Acad. Sci., 101, pp. 12940-12945
  • Varfolomeev, E.E., Ashkenazi, A., Tumor necrosis factor: an apoptosis JunKie? (2004) Cell, 116, pp. 491-497
  • Jupp, O.J., McFarlane, S.M., Anderson, H.M., Littlejohn, A.F., Mohamed, A.A., MacKay, R.H., Vandenabeele, P., MacEwan, D.J., Type II tumor necrosis factor-α (TNFR2) activates c-Jun N-terminal kinase (JNK) but not mitogen-activated protein kinase (MAPK) or p38 MAPK pathways (2001) Biochem. J., 359, pp. 525-535
  • Baker, D., Butler, D., Scallon, B.J., O, J.K., Neill, Turk, J.L., Feldmann, M., Control of established experimental allergic encephalomyelitis by inhibition of tumor necrosis factor (TNF) activity within the central nervous system using monoclonal antibodies and TNF receptor-immunoglobulin fusion proteins (1994) Eur. J. Immunol., 24, pp. 2040-2048
  • Sirén, A.L., Fratelli, M., Brines, M., Goemans, C., Casagrande, S., Lewczuk, P., Keenan, S., Ghezzi, P., Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress (2001) Proc. Natl. Acad. Sci. U. S. A., 98, pp. 4044-4049
  • Agnello, D., Bigini, P., Villa, P., Mennini, T., Cerami, A., Brines, M.L., Ghezzi, P., Erythropoietin exerts an anti-inflammatory effect on the CNS in a model of experimental autoimmune encephalomyelitis (2002) Brain Res., 952, pp. 128-134
  • Brines y, M., Cerami, A., Emerging biological roles for erythropoietin in the nervous system (2005) Nat. Rev. Neurosci., 6, pp. 484-494
  • Lowry, O.H., Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays (1983) J. Immunol. Methods, 65, pp. 55-63
  • Vittori, D., Pregi, N., Pérez, G., Garbossa, G., Nesse, A., The distinct erythropoietin functions that promote cell survival and proliferation are affected by aluminium exposure through mechanisms involving erythropoietin receptor (2005) Biochim. Biophys. Acta, 1743, pp. 29-36
  • Laemmli, U.K., Amos, L.A., Klug, A., Correlation between structural transformation and cleavage of the major head protein of T4 bacteriophage (1976) Cell, 7, pp. 191-203
  • Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) Anal. Biochem., 162, pp. 156-159
  • Benito, A., Silva, M., Grillot, D., Núñez, G., Fernández-Luna, J.L., Apoptosis induced by erythroid differentiation of human leukaemia cell lines is inhibited by Bcl-xL (1996) Blood, 87, pp. 3837-3843
  • McKinney, M., Robbins, M., Chronic atropine administration up-regulates rat cortical muscarinic m 1 receptor mRNA molecules: assessment with the RT/PCR (1992) Brain Res. Mol. Brain Res., 12, pp. 39-45
  • Yang, L., Lindholm, K., Konishi, Y., Li, R., Shen, Y., Target depletion of distinct tumor necrosis factor receptor subtypes reveals hippocampal neuron death and survival through different signal transduction pathways (2002) J. Neurosci., 22, pp. 3025-3032
  • Thorburn, Death receptor-induced cell killing (2004) Cell Signal., 16, pp. 139-144
  • Socolovsky, M., Fallon, A.E., Wang, S., Brugnara, C., Lodish, H.F., Fetal anemia and apoptosis of red cell progenitors in Stat5a-/-5b-/- mice: a direct role for Stat5 in Bcl-X(L) induction (1999) Cell, 98, pp. 181-191
  • Chong, Z.Z., Kang, J.-Q., Maiese, K., Erythropoietin fosters both intrinsic and extrinsic neuronal protection through modulation of microglia, Akt, Bad, and caspase-mediated pathways (2003) Br. J. Pharmacol., 138, pp. 1107-1118
  • Thommesen, L., Lægreid, A., Distinct differences between TNF receptor 1- and TNF Receptor 2-mediated activation of NFκB (2005) J. Biochem. Mol. Biol., 38, pp. 281-289
  • Nicolas, O., Gavin, R., Braun, N., Ureña, J.M., Fontana, X., Soriano, E., Aguzzi, A., del Río, J.A., Bcl-2 overexpression delays caspase-3 activation and rescues cerebellar degeneration in prion-deficient mice that overexpress amino-terminally truncated prion (2007) FASEB J., 21, pp. 3107-3117
  • Bittorf, T., Büchse, T., Sasse, T., Jaster, R., Brock, J., Activation of the transcription factor NF-κB by the erythropoietin receptor. Structural requirements and biological significance (2001) Cell Signal., 13, pp. 673-681
  • Chong, Z.Z., Li, F., Maiese, K., Erythropoietin requires NF-κB and its nuclear translocation to prevent early and late apoptotic neuronal injury during β-amyloid toxicity (2005) Curr. Neurovasc. Res., 2, pp. 387-399
  • Digicaylioglu, M., Lipton, S.A., Erythropoietin-mediated neuroprotection involves cross-talk between Jak2 and NF-kappaB signalling cascades (2001) Nature, 412, pp. 641-647
  • Micheau, O., Tschopp, J., Induction of TNF Receptor I-mediated apoptosis via two sequential signalling complexes (2003) Cell, 114, pp. 181-190
  • Krueger, A., Baumann, S., Krammer, P., Kirchhoff, S., FLICE-Inhibitory proteins: regulators of death receptor-mediated apoptosis (2001) Mol. Cell. Biol., 21, pp. 8247-8254
  • Rafiee, P., Shi, Y., Su, J., Pritchard, K.A., Tweddell, J.S., Baker, J.E., Erythropoietin protects the infant heart against ischemia-reperfusion injury by triggering multiple signaling pathways (2005) Basic Res. Cardiol., 100, pp. 187-197
  • Akhtar, R.S., Ness, J.M., Roth, K.A., Bcl-2 family regulation of neuronal development and neurodegeneration (2004) Biochim. Biophys. Acta, 1644, pp. 189-203
  • Maiese, K., Li, F., Chong, Z.Z., Erythropoietin in the brain: can the promise to protect be fulfilled? (2004) Trends Pharmacol. Sci., 25, pp. 577-583
  • Baeuerle, P.A., Baltimore, D., NF-kappa B: ten years after (1996) Cell, 87, pp. 13-20
  • Marchetti, L., Klein, M., Schlett, K., Pfizenmaier, K., Eisel, U.M.L., Tumor necrosis factor (TNF)-mediated neuroprotection against glutamate-induced excitotoxicity is enhanced by N-methyl-D-aspartate receptor activation (2004) J. Biol. Chem., 279, pp. 32861-32881
  • Taoufik, E., Petit, E., Divoux, D., Tseveleki, V., Mengozzi, M., Roberts, M., Valable, S., Probert, L., TNF receptor I sensitizes neurons to erythropoietin- and VEGF-mediated neuroprotection after ischemic and excitotoxic injury (2008) Proc. Natl. Acad. Sci. U. S. A., 105, pp. 6185-6190

Citas:

---------- APA ----------
Pregi, N., Wenker, S., Vittori, D., Leirós, C.P. & Nesse, A. (2009) . TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells. Experimental Cell Research, 315(3), 419-431.
http://dx.doi.org/10.1016/j.yexcr.2008.11.005
---------- CHICAGO ----------
Pregi, N., Wenker, S., Vittori, D., Leirós, C.P., Nesse, A. "TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells" . Experimental Cell Research 315, no. 3 (2009) : 419-431.
http://dx.doi.org/10.1016/j.yexcr.2008.11.005
---------- MLA ----------
Pregi, N., Wenker, S., Vittori, D., Leirós, C.P., Nesse, A. "TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells" . Experimental Cell Research, vol. 315, no. 3, 2009, pp. 419-431.
http://dx.doi.org/10.1016/j.yexcr.2008.11.005
---------- VANCOUVER ----------
Pregi, N., Wenker, S., Vittori, D., Leirós, C.P., Nesse, A. TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells. Exp. Cell Res. 2009;315(3):419-431.
http://dx.doi.org/10.1016/j.yexcr.2008.11.005