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Bustamante, J.; Guerra, L.; Bredeston, L.; Mordoh, J.; Boveris, A. "Melanin content and hydroperoxide metabolism in human melanoma cells" (1991) Experimental Cell Research. 196(2):172-176
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Abstract:

Human melanoma cells were grown to exponential and stationary phases showing melanin contents of 4.2 ± 0.3 and 11.3 ± 0.6 μg/10 6 cells, respectively. The cells were separated in four subpopulations by a Percoll gradient; the subpopulation of density 1.07 (g/ml) was the most enriched in pigmented cells and produced 28 and 58% of the cells in exponential and stationary phases, respectively. Melanoma cells had similar superoxide dismutase and glutathione peroxidase activities in exponential and stationary phases. Moreover melanoma cells exhibited a higher catalase activity in the stationary phase: whole homogenate and cytosol activities were 7.0 ± 0.3 and 10.8 ± 0.6 U/mg protein, whereas in exponential phase the activities were 4.9 ± 0.1 and 7.6 ± 0.3 U/mg protein for whole homogenate and cytosol, respectively. The intracellular H 2 O 2 steady-state concentration was 3.3 ± 0.2 and 2.1 ± 0.2 μM H 2 O 2 for exponential and stationary phases, respectively. The spontaneous chemiluminescence of the two culture phases was 169 ± 27 cps/10 6 cells (exponential) and 78 ± 24 cps/10 6 cells (stationary). The cytotoxicity of H 2 O 2 generated extracellularly by glucose oxidase was determined after 60 min of exposure. IC 50 values for exponential and stationary cell cultures were 0.9 and 2.4 mU/ml of glucose oxidase, respectively. The increased catalase activities in the stationary phase as compared with the exponential phase are consistent with the decreased intracellular H 2 O 2 , with the decreased spontaneous chemiluminescence, and with the increased resistance to exogenous H 2 O 2 . © 1991.

Registro:

Documento: Artículo
Título:Melanin content and hydroperoxide metabolism in human melanoma cells
Autor:Bustamante, J.; Guerra, L.; Bredeston, L.; Mordoh, J.; Boveris, A.
Filiación:Instituto de Quimica y Fisicoquimica Biologicas, Facultad de Farmacia y Bioquimica (UBA), Junin 956, 1113 Buenos Aires, Argentina
Instituto de Investigacions Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Palabras clave:catalase; hydroperoxide; melanin; article; cell growth; cell level; chemoluminescence; human; human cell; melanoma cell; priority journal; Catalase; Cell Division; Cell Line; Cell Survival; Cytosol; Glutathione Peroxidase; Human; Hydrogen Peroxide; Luminescence; Melanins; Melanoma; Oxygen Consumption; Superoxide Dismutase; Support, Non-U.S. Gov't
Año:1991
Volumen:196
Número:2
Página de inicio:172
Página de fin:176
DOI: http://dx.doi.org/10.1016/0014-4827(91)90247-R
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:Catalase, EC 1.11.1.6; Glutathione Peroxidase, EC 1.11.1.9; Hydrogen Peroxide, 7722-84-1; Melanins; Superoxide Dismutase, EC 1.15.1.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v196_n2_p172_Bustamante

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Citas:

---------- APA ----------
Bustamante, J., Guerra, L., Bredeston, L., Mordoh, J. & Boveris, A. (1991) . Melanin content and hydroperoxide metabolism in human melanoma cells. Experimental Cell Research, 196(2), 172-176.
http://dx.doi.org/10.1016/0014-4827(91)90247-R
---------- CHICAGO ----------
Bustamante, J., Guerra, L., Bredeston, L., Mordoh, J., Boveris, A. "Melanin content and hydroperoxide metabolism in human melanoma cells" . Experimental Cell Research 196, no. 2 (1991) : 172-176.
http://dx.doi.org/10.1016/0014-4827(91)90247-R
---------- MLA ----------
Bustamante, J., Guerra, L., Bredeston, L., Mordoh, J., Boveris, A. "Melanin content and hydroperoxide metabolism in human melanoma cells" . Experimental Cell Research, vol. 196, no. 2, 1991, pp. 172-176.
http://dx.doi.org/10.1016/0014-4827(91)90247-R
---------- VANCOUVER ----------
Bustamante, J., Guerra, L., Bredeston, L., Mordoh, J., Boveris, A. Melanin content and hydroperoxide metabolism in human melanoma cells. Exp. Cell Res. 1991;196(2):172-176.
http://dx.doi.org/10.1016/0014-4827(91)90247-R