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Abstract:

In order to study the roles of the different alternatively spliced variants of human fibronectin (FN) as well as of its binding sites and structural domains in the processes of extracellular matrix assembly, cell adhesion, and cell migration, we constructed expression vectors coding for different human full-length FN polypeptides and deleted versions of these constructs. We expressed them transiently in mammalian cells by calcium phosphate transfection and microinjection techniques. While the deleted recombinants were expressed by both transfection and microinjection, the full-length recombinants could be only expressed by microinjection. Calcium phosphate transfection leads to the accumulation of recombinant FN in cytoplasmic vesicles of both matrix-forming and nonforming cells. On the contrary, microinjection leads to the accumulation of recombinant FN in cytoplasmic vesicles in cells that do not form a matrix, but to the rapid incorporation into the extracellular matrix of matrix-forming cells in addition to a cytoplasmic localization. Identical results were obtained when the FN signal and propeptides were replaced by those of E-cadherin. © 1991.

Registro:

Documento: Artículo
Título:Generation of full-length cDNA recombinant vectors for the transient expression of human fibronectin in mammalian cell lines
Autor:Dufour, S.; Gutman, A.; Bois, F.; Lamb, N.; Thiery, J.P.; Kornblihtt, A.R.
Filiación:Laboratoire de Physiopathologie du Développement, CNRS URA 1337, Ecole Normale Supérieure, 46, rue d'Ulm, 75230 Paris Cedex 05, France
Centre de Recherche de Biochimie Macromoléculaire, CNRS-INSERM, 34033 Montpellier, France
INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:complementary dna; fibronectin; animal cell; article; cell adhesion; expression vector; extracellular matrix; human; mouse; priority journal; recombinant dna technology; Animal; Cloning, Molecular; DNA; Fibronectins; Fluorescent Antibody Technique; Gene Expression; Genetic Vectors; Human; Mice; Microinjections; Support, Non-U.S. Gov't; Transfection; Mammalia
Año:1991
Volumen:193
Número:2
Página de inicio:331
Página de fin:338
DOI: http://dx.doi.org/10.1016/0014-4827(91)90104-3
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:DNA, 9007-49-2; Fibronectins; Genetic Vectors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v193_n2_p331_Dufour

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Citas:

---------- APA ----------
Dufour, S., Gutman, A., Bois, F., Lamb, N., Thiery, J.P. & Kornblihtt, A.R. (1991) . Generation of full-length cDNA recombinant vectors for the transient expression of human fibronectin in mammalian cell lines. Experimental Cell Research, 193(2), 331-338.
http://dx.doi.org/10.1016/0014-4827(91)90104-3
---------- CHICAGO ----------
Dufour, S., Gutman, A., Bois, F., Lamb, N., Thiery, J.P., Kornblihtt, A.R. "Generation of full-length cDNA recombinant vectors for the transient expression of human fibronectin in mammalian cell lines" . Experimental Cell Research 193, no. 2 (1991) : 331-338.
http://dx.doi.org/10.1016/0014-4827(91)90104-3
---------- MLA ----------
Dufour, S., Gutman, A., Bois, F., Lamb, N., Thiery, J.P., Kornblihtt, A.R. "Generation of full-length cDNA recombinant vectors for the transient expression of human fibronectin in mammalian cell lines" . Experimental Cell Research, vol. 193, no. 2, 1991, pp. 331-338.
http://dx.doi.org/10.1016/0014-4827(91)90104-3
---------- VANCOUVER ----------
Dufour, S., Gutman, A., Bois, F., Lamb, N., Thiery, J.P., Kornblihtt, A.R. Generation of full-length cDNA recombinant vectors for the transient expression of human fibronectin in mammalian cell lines. Exp. Cell Res. 1991;193(2):331-338.
http://dx.doi.org/10.1016/0014-4827(91)90104-3