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Abstract:

The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2a, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2a has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate. © 1989.

Registro:

Documento: Artículo
Título:Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants
Autor:Resnicoff, M.; Medrano, E.E.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Palabras clave:13 cis retinal; butyric acid; estradiol; insulin; prostaglandin f2 alpha; radioisotope; thrombin; unclassified drug; breast cancer; cell culture; cell differentiation; cell growth; cell strain mcf 7; controlled study; cytology; female; human; human cell; priority journal; Breast Neoplasms; Butyric Acid; Butyric Acids; Cell Differentiation; Cell Division; Cell Survival; Culture Media; Dinoprost; DNA; Estradiol; Hormones; Human; Insulin; Retinaldehyde; Retinoids; Support, Non-U.S. Gov't; Thrombin; Tumor Cells, Cultured; Tumor Stem Cells
Año:1989
Volumen:181
Número:1
Página de inicio:116
Página de fin:125
DOI: http://dx.doi.org/10.1016/0014-4827(89)90187-0
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:butyric acid, 107-92-6, 156-54-7, 461-55-2; estradiol, 50-28-2; insulin, 9004-10-8; prostaglandin F2 alpha, 551-11-1; thrombin, 9002-04-4; 13-cis-retinal, 472-86-6; Butyric Acid, 107-92-6; Butyric Acids; Culture Media; Dinoprost, 551-11-1; DNA, 9007-49-2; Estradiol, 50-28-2; Hormones; Insulin, 11061-68-0; Retinaldehyde, 116-31-4; Retinoids; Thrombin, EC 3.4.21.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v181_n1_p116_Resnicoff

Referencias:

  • Hepner, (1984) Cancer Res, 44, pp. 2259-2265
  • Buick, Pollack, (1984) Cancer Res, 44, pp. 4909-4918
  • Resnicoff, Medrano, Podhajcer, Bravo, Bover, Mordoh, (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 7295-7299
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  • Medrano, E. E., Resnicoff, M., Larcher, F., Cafferata, E. G. A., Podhajcer, O. L., Bover, L., and Molinari, submitted for publication; Resnicoff, Medrano, (1987) Biochem. Biophys. Res. Commun, 143, pp. 309-315
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Citas:

---------- APA ----------
Resnicoff, M. & Medrano, E.E. (1989) . Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants. Experimental Cell Research, 181(1), 116-125.
http://dx.doi.org/10.1016/0014-4827(89)90187-0
---------- CHICAGO ----------
Resnicoff, M., Medrano, E.E. "Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants" . Experimental Cell Research 181, no. 1 (1989) : 116-125.
http://dx.doi.org/10.1016/0014-4827(89)90187-0
---------- MLA ----------
Resnicoff, M., Medrano, E.E. "Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants" . Experimental Cell Research, vol. 181, no. 1, 1989, pp. 116-125.
http://dx.doi.org/10.1016/0014-4827(89)90187-0
---------- VANCOUVER ----------
Resnicoff, M., Medrano, E.E. Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants. Exp. Cell Res. 1989;181(1):116-125.
http://dx.doi.org/10.1016/0014-4827(89)90187-0