The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2a, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2a has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate. © 1989.
Documento: | Artículo |
Título: | Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
Autor: | Resnicoff, M.; Medrano, E.E. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Patricias Argentinas 435, 1405 Buenos Aires, Argentina |
Palabras clave: | 13 cis retinal; butyric acid; estradiol; insulin; prostaglandin f2 alpha; radioisotope; thrombin; unclassified drug; breast cancer; cell culture; cell differentiation; cell growth; cell strain mcf 7; controlled study; cytology; female; human; human cell; priority journal; Breast Neoplasms; Butyric Acid; Butyric Acids; Cell Differentiation; Cell Division; Cell Survival; Culture Media; Dinoprost; DNA; Estradiol; Hormones; Human; Insulin; Retinaldehyde; Retinoids; Support, Non-U.S. Gov't; Thrombin; Tumor Cells, Cultured; Tumor Stem Cells |
Año: | 1989 |
Volumen: | 181 |
Número: | 1 |
Página de inicio: | 116 |
Página de fin: | 125 |
DOI: | http://dx.doi.org/10.1016/0014-4827(89)90187-0 |
Título revista: | Experimental Cell Research |
Título revista abreviado: | Exp. Cell Res. |
ISSN: | 00144827 |
CODEN: | ECREA |
CAS: | butyric acid, 107-92-6, 156-54-7, 461-55-2; estradiol, 50-28-2; insulin, 9004-10-8; prostaglandin F2 alpha, 551-11-1; thrombin, 9002-04-4; 13-cis-retinal, 472-86-6; Butyric Acid, 107-92-6; Butyric Acids; Culture Media; Dinoprost, 551-11-1; DNA, 9007-49-2; Estradiol, 50-28-2; Hormones; Insulin, 11061-68-0; Retinaldehyde, 116-31-4; Retinoids; Thrombin, EC 3.4.21.5 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v181_n1_p116_Resnicoff |