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Abstract:

The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988.

Registro:

Documento: Artículo
Título:Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
Autor:Podhajcer, O.L.; Resnicoff, M.; Bover, L.; Medrano, E.E.; Slavutsky, I.; Larripa, I.; Mordoh, J.
Filiación:Hospital Mariano R. Castex, Balcarce 900, 1650 San Martin, Provincia de Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Argentina
Academia Nacional de Medicina, Coronel Diaz, 2497, 1425 Buenos Aires, Argentina
Palabras clave:estradiol; tamoxifen; anchorage independent growth; cell culture; cell growth; cell strain mcf 7; controlled study; cytology; female; human; human cell; karyotyping; priority journal; stem cell; ultrastructure; Breast Neoplasms; Cell Adhesion; Cell Division; Cell Line; Estradiol; Genetic Markers; Human; Karyotyping; Support, Non-U.S. Gov't; Tamoxifen; Tumor Stem Cells
Año:1988
Volumen:179
Número:1
Página de inicio:58
Página de fin:64
DOI: http://dx.doi.org/10.1016/0014-4827(88)90348-5
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:estradiol, 50-28-2; tamoxifen, 10540-29-1; Estradiol, 50-28-2; Genetic Markers; Tamoxifen, 10540-29-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v179_n1_p58_Podhajcer

Referencias:

  • Buick, Pollack, (1984) Cancer Res, 44, pp. 4909-4918
  • Resnicoff, Medrano, Podhajcer, Bravo, Bover, Mordoh, (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 7295-7299
  • Suele, Vazquez, Long, Alberty, Brennan, (1973) J. Natl. Cancer Inst, 51, pp. 1409-1415
  • Barkley Butter, Berlinski, Hillman, Kelsey, Toenniges, (1986) Cancer Res, 46, pp. 6339-6348
  • Yang, Soule, McGrath, (1977) J. Natl. Cancer Inst, 59, pp. 1357-1367
  • Shin, Freedman, Risser, Pollack, (1975) Proc. Natl. Acad. Sci. USA, 72, pp. 4435-4439
  • Kahn, Shin, (1979) J. Cell Biol, 82, pp. 1-16
  • Smith, (1979) J. Natl. Cancer Inst, 62, pp. 225-230
  • Hamburger, Salmon, (1977) Science, 197, pp. 461-463
  • Seabright, (1971) Lancet, 2, pp. 971-972
  • ISCN, An International System for Human Cytogenetic Nomenclature (1978) (1978) Birth Defects: Original Article Series, 14. , The National Foundation, New York, No. 8
  • ISCN, (1978) Cytogenetic and Genome Research, 21, pp. 309-404. , also in
  • Medrano, E. E., Resnicoff, M., Larcher, F., Cafferata, E. G. A., Podhajcer, O., Bover, L., and Molinari, B. Submitted for publication; Whang-Peng, Lee, Kao-Shan, Seibert, Lippman, (1983) J. Natl. Cancer Inst, 71, pp. 687-695
  • Human Gene Mapping 8 (1985) Eight International Workshop on Human Gene Mapping, 40, pp. 1-4. , 2nd ed., Cytogenet. Cell Genet
  • Podhajcer, Bravo, Sorin, Guman, Cerdeiro, Mordoh, (1986) Cancer, 58, pp. 720-729

Citas:

---------- APA ----------
Podhajcer, O.L., Resnicoff, M., Bover, L., Medrano, E.E., Slavutsky, I., Larripa, I. & Mordoh, J. (1988) . Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation. Experimental Cell Research, 179(1), 58-64.
http://dx.doi.org/10.1016/0014-4827(88)90348-5
---------- CHICAGO ----------
Podhajcer, O.L., Resnicoff, M., Bover, L., Medrano, E.E., Slavutsky, I., Larripa, I., et al. "Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation" . Experimental Cell Research 179, no. 1 (1988) : 58-64.
http://dx.doi.org/10.1016/0014-4827(88)90348-5
---------- MLA ----------
Podhajcer, O.L., Resnicoff, M., Bover, L., Medrano, E.E., Slavutsky, I., Larripa, I., et al. "Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation" . Experimental Cell Research, vol. 179, no. 1, 1988, pp. 58-64.
http://dx.doi.org/10.1016/0014-4827(88)90348-5
---------- VANCOUVER ----------
Podhajcer, O.L., Resnicoff, M., Bover, L., Medrano, E.E., Slavutsky, I., Larripa, I., et al. Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation. Exp. Cell Res. 1988;179(1):58-64.
http://dx.doi.org/10.1016/0014-4827(88)90348-5