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Abstract:

The exposure of serum-deprived mammary tumor cells MCF-7 and T-47D to insulin, thrombin, and epidermal growth factor (EGF) resulted in dramatic modifications in the activity and in the translocation capacity of protein kinase C from cytosol to membrane fractions. Insulin induces a 600% activation of the enzyme after 5 h of exposure to the hormone in MCF-7 cells; thrombin either activates (200% in MCF-7) or down-regulates (in T-47D), and EGF exerts only a moderate effect. Thus, the growth factors studied modulate differentially the protein kinase C activity in human mammary tumor cells. The physiological significance of the results obtained are discussed in terms of the growth response elicited by insulin, thrombin, and EGF. © 1988.

Registro:

Documento: Artículo
Título:Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
Autor:Gomez, M.L.; Medrano, E.E.; Cafferatta, E.G.A.; Tellez-Inon, M.T.
Filiación:Instituto de Ingeniería Genética y Biología Molecular (INGEBI, CONICET), Argentina
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales (UBA), Buenos Aires, Argentina
Palabras clave:epidermal growth factor; insulin; protein kinase c; thrombin; breast tumor; cell culture; human; human cell; Breast Neoplasms; Cell Division; Cell Membrane; Cytoplasm; Epidermal Growth Factor; Human; Insulin; Protein Kinase C; Support, Non-U.S. Gov't; Thrombin; Tumor Cells, Cultured
Año:1988
Volumen:175
Número:1
Página de inicio:74
Página de fin:80
DOI: http://dx.doi.org/10.1016/0014-4827(88)90256-X
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:epidermal growth factor, 62229-50-9; insulin, 9004-10-8; protein kinase C, 141436-78-4; thrombin, 9002-04-4; Epidermal Growth Factor, 62229-50-9; Insulin, 11061-68-0; Protein Kinase C, EC 2.7.1.37; Thrombin, EC 3.4.21.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v175_n1_p74_Gomez

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Citas:

---------- APA ----------
Gomez, M.L., Medrano, E.E., Cafferatta, E.G.A. & Tellez-Inon, M.T. (1988) . Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells. Experimental Cell Research, 175(1), 74-80.
http://dx.doi.org/10.1016/0014-4827(88)90256-X
---------- CHICAGO ----------
Gomez, M.L., Medrano, E.E., Cafferatta, E.G.A., Tellez-Inon, M.T. "Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells" . Experimental Cell Research 175, no. 1 (1988) : 74-80.
http://dx.doi.org/10.1016/0014-4827(88)90256-X
---------- MLA ----------
Gomez, M.L., Medrano, E.E., Cafferatta, E.G.A., Tellez-Inon, M.T. "Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells" . Experimental Cell Research, vol. 175, no. 1, 1988, pp. 74-80.
http://dx.doi.org/10.1016/0014-4827(88)90256-X
---------- VANCOUVER ----------
Gomez, M.L., Medrano, E.E., Cafferatta, E.G.A., Tellez-Inon, M.T. Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells. Exp. Cell Res. 1988;175(1):74-80.
http://dx.doi.org/10.1016/0014-4827(88)90256-X