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Abstract:

The activities of topoisomerases I and II were assayed in subcellular extracts obtained from nontumorigenic BALB/c 3T3 A31 and normal rat kidney (NRK) cell lines and from the same cells transformed by benzo[a]pyrene (BP-A31), Moloney (M-MSV-A31) and Kirsten (K-A31) sarcoma viruses, and simian virus 40 (SV-NRK). The enzymatic activity of topoisomerase I was monitored by the relaxation of negatively supercoiled pBR322 DNA and by the formation of covalent complexes between 32P-labeled DNA and topoisomerase I. Topoisomerase II activity was determined by decatenation of kinetoplast DNA (k-DNA). It was found that nuclear and cytoplasmic type I topoisomerase specific activities were higher in every transformed cell line than in the normal counterparts. These differences cannot be attributed to an inhibitory factor present in A31 cells. When chromatin was treated at increasing ionic strengths, the 0.4 M NaCl extract showed the highest topoisomerase I specific activity. Moreover, in this fraction the transformed cells exhibited the most significant increment in the enzymatic activity as compared with nontransformed cultures. Spontaneously transformed A31 cells showed topoisomerase I activity similar to that of extracts of cells transformed by benzo[a]pyrene. Topoisomerase II specific activity was also increased in SV-NRK cells, as judged by the assay for decatenation of k-DNA to yield minicircle DNA. © 1988.

Registro:

Documento: Artículo
Título:Increment of DNA topoisomerases in chemically and virally transformed cells
Autor:Crespi, M.D.; Mladovan, A.G.; Baldi, A.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:benzo[a]pyrene; isomerase; radioisotope; animal cell; cell culture; kidney; mouse; nonhuman; rat; sarcoma virus; simian virus; Animal; Benzo(a)pyrene; Cell Adhesion; Cell Nucleus; Cell Transformation, Neoplastic; Cell Transformation, Viral; DNA Topoisomerases, Type I; Mice; Novobiocin; Rats; Support, Non-U.S. Gov't
Año:1988
Volumen:175
Número:1
Página de inicio:206
Página de fin:215
DOI: http://dx.doi.org/10.1016/0014-4827(88)90267-4
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:benzo[a]pyrene, 50-32-8; isomerase, 9013-19-8; Benzo(a)pyrene, 50-32-8; DNA Topoisomerases, Type I, EC 5.99.1.2; Novobiocin, 303-81-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v175_n1_p206_Crespi

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Citas:

---------- APA ----------
Crespi, M.D., Mladovan, A.G. & Baldi, A. (1988) . Increment of DNA topoisomerases in chemically and virally transformed cells. Experimental Cell Research, 175(1), 206-215.
http://dx.doi.org/10.1016/0014-4827(88)90267-4
---------- CHICAGO ----------
Crespi, M.D., Mladovan, A.G., Baldi, A. "Increment of DNA topoisomerases in chemically and virally transformed cells" . Experimental Cell Research 175, no. 1 (1988) : 206-215.
http://dx.doi.org/10.1016/0014-4827(88)90267-4
---------- MLA ----------
Crespi, M.D., Mladovan, A.G., Baldi, A. "Increment of DNA topoisomerases in chemically and virally transformed cells" . Experimental Cell Research, vol. 175, no. 1, 1988, pp. 206-215.
http://dx.doi.org/10.1016/0014-4827(88)90267-4
---------- VANCOUVER ----------
Crespi, M.D., Mladovan, A.G., Baldi, A. Increment of DNA topoisomerases in chemically and virally transformed cells. Exp. Cell Res. 1988;175(1):206-215.
http://dx.doi.org/10.1016/0014-4827(88)90267-4