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Abstract:

The growth of the human metastatic cell line (T-47D) in a chemically defined medium (DM) is shown to be dependent on the presence of three traditional growth factors: epidermal growth factor, insulin, and transferrin. The addition of thrombin further stimulates its growth. The mitogenic action on a human mammary tumor cell line from epithelial origin is a novel action of thrombin. Cells in the DM show striking morphological changes which are dramatically enhanced by the addition of thrombin. These observations are part of a pleiotropic response to the growth factors: the protein content of the cells increases in the defined medium; the 2DG gels of the 35S- and 32P-labeled proteins show important changes in spots, several of which are probably of cytoskeletal origin. It is also shown that cells in a semisolid growth factor-supplemented medium have growth advantages over their counterparts grown with serum. All the phenotypic changes mentioned above reveal the important role of growth factors in the growth and behavior of this mammary cell line. The results obtained with thrombin indicate a new site of action of this enzyme which may be important in the metastatic spread of human mammary tumor cells. © 1987.

Registro:

Documento: Artículo
Título:Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
Autor:Medrano, E.E.; Cafferata, E.G.A.; Larcher, F.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Comisión Nacional de Energía Atómica, Buenos Aires, Argentina
Palabras clave:growth factor; thrombin; cell growth; human; human cell; metastasis; protein synthesis; tumor cell; Breast Neoplasms; Cell Division; Culture Media; DNA Replication; Drug Synergism; Epidermal Growth Factor; Gene Expression Regulation; Human; Insulin; Neoplasm Proteins; Phosphorylation; Sepharose; Support, Non-U.S. Gov't; Thrombin; Transferrin; Tumor Cells, Cultured
Año:1987
Volumen:172
Número:2
Página de inicio:354
Página de fin:364
DOI: http://dx.doi.org/10.1016/0014-4827(87)90393-4
Título revista:Experimental Cell Research
Título revista abreviado:Exp. Cell Res.
ISSN:00144827
CODEN:ECREA
CAS:thrombin, 9002-04-4; Culture Media; Epidermal Growth Factor, 62229-50-9; Insulin, 11061-68-0; Neoplasm Proteins; Sepharose, 9012-36-6; Thrombin, EC 3.4.21.5; Transferrin, 11096-37-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v172_n2_p354_Medrano

Referencias:

  • Becker, Poupon, Courtois, (1984) Biol. Cell, 50, p. 20a
  • Keydar, Chen, Karby, Weiss, Delarea, Radu, Chaitcick, Brenner, (1979) Eur. J. Cancer, 15, p. 659
  • Gospodarowicz, Brown, Birdwell, Zetter, (1978) J. Cell Biol, 77, p. 774
  • Carney, Cunningham, (1978) Cell, 15, p. 1341
  • Carney, Cunningham, (1978) Cell, 14, p. 811
  • Perdue, Lubensky, Kivity, Sonder, Fenton, (1981) J. Biol. Chem, 256, p. 2767
  • Carney, Stiernberg, Fenton, (1984) J. Cell Biochem, 26, p. 181
  • Carney, (1983) J. Cell. Physiol, 117, p. 297
  • Carney, Scott, Gordonn, LaBelle, (1985) Cell, 42, p. 479
  • Sato, (1980) Hormones and Cancer, p. 281. , S. Iacobelli et al., Raven, New York
  • Medrano, Goldemberg, Algranati, Differential effect of ?difluoromethyl-ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells (1983) Journal of Cellular Physiology, 117, p. 117
  • O'Farrell, (1975) J. Biol. Chem, 250, p. 4007
  • Laemmli, (1970) Nature (London), 227, p. 680
  • Cherington, Pardee, (1980) J. Cell. Physiol, 105, p. 25
  • Osborne, Hamilton, Nover, (1981) Proc. Amer. Assoc. Cancer Res, 40, p. 2361. , 2nd edition
  • Imagawa, Tomooka, Nandi, (1982) Proc. Natl. Acad. Sci. USA, 79, p. 4074. , 2nd edition
  • Imai, Leung, Friesen, Shin, (1982) Cancer Res, 42, p. 4394
  • Pardee, Cherington, Medrano, (1981) Control of Cellular Division and Development, p. 495. , A. R. Liss, New York, Part A
  • Campisi, Medrano, Morreo, Pardee, (1982) Proc. Natl. Acad. Sci.USA, 79, p. 436. , 2nd edition
  • Bernal, Chen, (1982) Cancer Res, 42, p. 5106
  • Hunter, (1980) Cell, 22, p. 647
  • Hynes, (1982) Cell, 28, p. 437
  • Heldin, Westermark, (1984) Cell, 37, p. 9
  • Yuhas, Tarleton, Molzen, (1978) Cancer Res, 38, p. 2486
  • Pledger, Stiles, Antoniades, Scher, Induction of DNA synthesis in BALB/c 3T3 cells by serum components: Reevaluation of the commitment process (1983) Proceedings of the National Academy of Sciences, 74, p. 4481. , 2nd edition
  • Van Obberghen-Schilling, Perez-Rodriguez, Pouyssegur, (1982) J. Biochem. Biophys. Res. Commun, 106, p. 79
  • Baserga, (1984) Biol. Cell, 50, p. 2a
  • Epifanova, Int. Rev. Cytol. Suppl. I (1977) Int. Rev. Cytol. Suppl. I, p. 303
  • Folkman, Greenspan, (1975) Biochim. Biophys Acta, 417, p. 217
  • Farmer, Wan, Ben-Ze'ev, Penman, (1983) Mol. Cell. Biol, 3, p. 182
  • Brinkley, Beall, Wible, Mace, Turner, Cailleau, (1980) Cancer Res, 40, p. 3118
  • Bockus, Stiles, (1984) Exp. Cell Res, 153, p. 186
  • Freeman, Shin, (1974) Cell, 3, p. 355
  • Khan, Shin, Cellular tumorigenicity in nude mice. Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability (1979) The Journal of Cell Biology, 82, p. 1
  • Harrington, Godman, (1980) Proc. Natl. Acad. Sci USA, 77, p. 423. , 2nd edition
  • Sakagami, Manabe, (1984) Biochim. Biophys. Acta, 120, p. 520
  • Massague, The transforming growth factors (1985) Trends in Biochemical Sciences, 10, p. 237
  • Medrano, E. E., Larcher, F., Resnicoff, M., and Cafferata, E. G. A., submitted for publication; Kaplan, Ozanne, (1983) Cell, 33, p. 931
  • Folkman, (1975) Ann. Intern Med, 82, p. 96
  • Lowry, Rosebrough, Fair, Randall, (1951) J. Biol. Chem, 193, p. 265

Citas:

---------- APA ----------
Medrano, E.E., Cafferata, E.G.A. & Larcher, F. (1987) . Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin. Experimental Cell Research, 172(2), 354-364.
http://dx.doi.org/10.1016/0014-4827(87)90393-4
---------- CHICAGO ----------
Medrano, E.E., Cafferata, E.G.A., Larcher, F. "Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin" . Experimental Cell Research 172, no. 2 (1987) : 354-364.
http://dx.doi.org/10.1016/0014-4827(87)90393-4
---------- MLA ----------
Medrano, E.E., Cafferata, E.G.A., Larcher, F. "Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin" . Experimental Cell Research, vol. 172, no. 2, 1987, pp. 354-364.
http://dx.doi.org/10.1016/0014-4827(87)90393-4
---------- VANCOUVER ----------
Medrano, E.E., Cafferata, E.G.A., Larcher, F. Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin. Exp. Cell Res. 1987;172(2):354-364.
http://dx.doi.org/10.1016/0014-4827(87)90393-4