Artículo
Abstract:
Rat substantia nigra slices were superfused with a physiological medium containing a diluted substance P (SP) antiserum, bacitracin and serum albumin to measure SP released in superfusate. As shown by measuring the degradation of a SP-labelled derivative incubated with cerebellar slices, the medium prevented the enzymatic inactivation of SP. Potassium (K+, 50 mM) and veratridine (5 x 10-5 M) stimulated SP release and these effects were respectively prevented in absence of calcium and in presence of tetrodotoxin ( 5 x 10-7 M). GABA (5 x 10-5 M), nicotine (5 x 10-6 M) and L-glutamic acid (5 x 10-5 M) reduced the K+ (50 mM)-evoked release of SP. In contrast, glycine (5 x 10-5 M), oxotremorine (5 x 10-5 M), D-glutamic acid (5 x 10-5 M) and serotonin (5 x 10-5 M) were without effect. Pempidine (5 x 10-5 M) prevented the inhibitory effect of nicotine (5 x 10-6 M) on the K+-evoked release of SP. Glutamic acid diethyl ester (10-4 M) completely abolished the L-glutamic acid-induced inhibition of the K+-evoked release of SP. Picrotoxin (5 x 10-5 M) did not influence the L-glutamic acid inhibitory effect excluding the intervention of GABAergic mechanisms.
Registro:
| Documento: | Artículo |
| Título: | Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat |
| Autor: | Borda, E.; Del Carmen Agostini, M.; Sterin-Borda, L.; Gimeno, M.F.; Gimeno, A.L. |
| Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET), V. de Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | 4 aminobutyric acid; glutamic acid; glutamic acid diethyl ester; glycine; nicotine; oxotremorine; pempidine; picrotoxin; potassium; serotonin; substance P; tetrodotoxin; veratridine; animal experiment; brain slice; central nervous system; controlled study; drug comparison; in vitro study; rat; substantia nigra |
| Año: | 1981 |
| Volumen: | 71 |
| Número: | 4 |
| Página de inicio: | 383 |
| Página de fin: | 392 |
| DOI: | http://dx.doi.org/10.1016/0014-2999(81)90601-4 |
| Título revista: | European Journal of Pharmacology |
| Título revista abreviado: | EUR. J. PHARMACOL. |
| ISSN: | 00142999 |
| CODEN: | EJPHA |
| CAS: | 4 aminobutyric acid, 28805-76-7, 56-12-2; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; glutamic acid diethyl ester, 16450-41-2; glycine, 56-40-6, 6000-43-7, 6000-44-8; nicotine, 54-11-5; oxotremorine, 70-22-4; pempidine, 79-55-0; picrotoxin, 124-87-8; potassium, 7440-09-7; serotonin, 50-67-9; substance P, 33507-63-0; tetrodotoxin, 4368-28-9, 4664-41-9; veratridine, 71-62-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v71_n4_p383_Borda |
Citas:
---------- APA ----------
Borda, E., Del Carmen Agostini, M., Sterin-Borda, L., Gimeno, M.F. & Gimeno, A.L. (1981) . Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat. European Journal of Pharmacology, 71(4), 383-392.http://dx.doi.org/10.1016/0014-2999(81)90601-4
---------- CHICAGO ----------
Borda, E., Del Carmen Agostini, M., Sterin-Borda, L., Gimeno, M.F., Gimeno, A.L. "Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat" . European Journal of Pharmacology 71, no. 4 (1981) : 383-392.http://dx.doi.org/10.1016/0014-2999(81)90601-4
---------- MLA ----------
Borda, E., Del Carmen Agostini, M., Sterin-Borda, L., Gimeno, M.F., Gimeno, A.L. "Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat" . European Journal of Pharmacology, vol. 71, no. 4, 1981, pp. 383-392.http://dx.doi.org/10.1016/0014-2999(81)90601-4
---------- VANCOUVER ----------
Borda, E., Del Carmen Agostini, M., Sterin-Borda, L., Gimeno, M.F., Gimeno, A.L. Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat. EUR. J. PHARMACOL. 1981;71(4):383-392.http://dx.doi.org/10.1016/0014-2999(81)90601-4
