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Abstract:

Muscarinic receptors play an important role in secretory and vasodilator responses in rat salivary glands. Nitric oxide synthase (NOS) activity was found coupled to muscarinic receptor activation as well as to nitric oxide-mediated amylase secretion elicited by carbachol. Parotid glands presented a predominant M3 and a minor muscarinic M1 acetylcholine receptor population, though carbachol stimulated NOS activity only through muscarinic M3 receptors as revealed in the presence of 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. Amylase secretion induced by carbachol appeared to be partly mediated by nitric oxide and nitric oxide-induced signaling since N-nitro-L-arginine methyl ester (L-NAME) inhibited the effect as well as did methylene blue. A negative regulation of NOS by protein kinase C activation in the presence of a high concentration of carbachol was seen in parotid glands and this inhibition was paralleled by amylase secretion. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland
Autor:Rosignoli, F.; Pérez Leirós, C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, 4 piso, (1428) Buenos Aires, Argentina
Palabras clave:Amylase secretion; Nitric oxide (NO) signaling; Nitric oxide (NO) synthase; Parotid gland; 4 diphenylacetoxy 1 methylpiperidine; amylase; carbachol; methylene blue; muscarinic M1 receptor; muscarinic M3 receptor; n(g) nitroarginine methyl ester; nitric oxide; nitric oxide synthase; phorbol 13 acetate 12 myristate; pirenzepine; protein kinase C; amylase release; animal tissue; article; concentration response; controlled study; drug mechanism; enzyme activation; enzyme activity; enzyme inhibition; male; nonhuman; parotid gland; priority journal; rat; receptor density; signal transduction; Amylases; Animals; Atropine; Binding, Competitive; Carbachol; Cell Membrane; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Inhibitors; Male; Muscarinic Antagonists; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Synthase; Parotid Gland; Piperidines; Pirenzepine; Quinuclidinyl Benzilate; Rats; Rats, Wistar; Receptors, Muscarinic; Specific Pathogen-Free Organisms
Año:2002
Volumen:439
Número:1-3
Página de inicio:27
Página de fin:33
DOI: http://dx.doi.org/10.1016/S0014-2999(02)01375-4
Título revista:European Journal of Pharmacology
Título revista abreviado:Eur. J. Pharmacol.
ISSN:00142999
CODEN:EJPHA
CAS:4-diphenylacetoxy-1,1-dimethylpiperidinium, 81405-11-0; Amylases, EC 3.2.1.-; Atropine, 51-55-8; Carbachol, 51-83-2; Enzyme Inhibitors; Muscarinic Antagonists; NG-Nitroarginine Methyl Ester, 50903-99-6; Nitric Oxide Synthase, EC 1.14.13.39; Nitric Oxide, 10102-43-9; Piperidines; Pirenzepine, 28797-61-7; Quinuclidinyl Benzilate, 6581-06-2; Receptors, Muscarinic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v439_n1-3_p27_Rosignoli

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Citas:

---------- APA ----------
Rosignoli, F. & Pérez Leirós, C. (2002) . Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland. European Journal of Pharmacology, 439(1-3), 27-33.
http://dx.doi.org/10.1016/S0014-2999(02)01375-4
---------- CHICAGO ----------
Rosignoli, F., Pérez Leirós, C. "Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland" . European Journal of Pharmacology 439, no. 1-3 (2002) : 27-33.
http://dx.doi.org/10.1016/S0014-2999(02)01375-4
---------- MLA ----------
Rosignoli, F., Pérez Leirós, C. "Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland" . European Journal of Pharmacology, vol. 439, no. 1-3, 2002, pp. 27-33.
http://dx.doi.org/10.1016/S0014-2999(02)01375-4
---------- VANCOUVER ----------
Rosignoli, F., Pérez Leirós, C. Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland. Eur. J. Pharmacol. 2002;439(1-3):27-33.
http://dx.doi.org/10.1016/S0014-2999(02)01375-4