Artículo

Brodsky, A.; Davio, C.; Shayo, C.; Legnazzi, B.L.; Barbosa, M.; Lardo, M.; Morelli, A.; Baldi, A.; Avalos, J.C.S.; Rivera, E. "Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation" (1998) European Journal of Pharmacology. 350(1):121-127
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The present study examines the effects of forskolin on U937 cell differentiation. We recently reported that dibutyryl cAMP (dbcAMP), but not cAMP-elevating agents such as histamine, promotes U937 cell differentiation. cAMP production elicited by stimulation of histamine H 2 receptors showed a rapid, homologous desensitization, which might explain the dissimilar responses to histamine and dbcAMP. Forskolin induced an increase in cAMP levels in a concentration-dependent manner (EC 50 = 30 μM) for an extended period of at least 24 h. Forskolin but not histamine (up to 100 μM), also inhibited cell growth in a dose-dependent fashion (EC 50 = 22 μM). After 3 days of incubation, 75 μM forskolin induced U937 cell differentiation as judged by an increased rate of reduction of nitrobluetetrazolium (mean ± S.E.M.: 21.3 ± 6.6% in treated cells vs. 3.2 ± 1.9% in the control group, P < 0.001) and an augmented chemotactic response to complement 5a (C5a) (33.2 ± 5.9% in forskolin-treated vs. 0.34 ± 0.12% in control cells, P < 0.01). Furthermore, c-Myc levels decreased following forskolin treatment, while the histamine H2 receptor agonist dimaprit had no effect. We conclude that forskolin induces U937 cell differentiation through a sustained rise in cAMP levels.

Registro:

Documento: Artículo
Título:Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation
Autor:Brodsky, A.; Davio, C.; Shayo, C.; Legnazzi, B.L.; Barbosa, M.; Lardo, M.; Morelli, A.; Baldi, A.; Avalos, J.C.S.; Rivera, E.
Filiación:Hematology Division, Jose de San Martin Clin. Hosp., 1113 Buenos Aires, Argentina
Radioisotopes Laboratories, University of Buenos Aires, 1113 Buenos Aires, Argentina
Immunogenetic Service, Jose de San Martin Clin. Hosp., 1113 Buenos Aires, Argentina
Hematology Laboratories, University of Buenos Aires, 1113 Buenos Aires, Argentina
Inst. of Biol./Experimental Medicine, Natl. Research Council of Argentine, 1428 Buenos Aires, Argentina
Palabras clave:CAMP; Cell differentiation; Desensitization; Forskolin; Histamine; U937 cell; complement component C5a; cyclic AMP; dimaprit; forskolin; histamine; histamine H2 receptor; Myc protein; antigen expression; article; cell differentiation; cell level; cell line; cell proliferation; chemotaxis; concentration response; controlled study; dose response; drug effect; growth inhibition; priority journal; receptor down regulation; receptor occupancy; respiratory burst; Antigens, Neoplasm; Cell Differentiation; Cell Division; Cell Line; Chemotaxis; Cyclic AMP; Dose-Response Relationship, Drug; Forskolin; Genes, myc; Histamine; Humans; Oxidation-Reduction; Tumor Cells, Cultured
Año:1998
Volumen:350
Número:1
Página de inicio:121
Página de fin:127
DOI: http://dx.doi.org/10.1016/S0014-2999(98)00227-1
Título revista:European Journal of Pharmacology
Título revista abreviado:Eur. J. Pharmacol.
ISSN:00142999
CODEN:EJPHA
CAS:Antigens, Neoplasm; Cyclic AMP, 60-92-4; Forskolin, 66428-89-5; Histamine, 51-45-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v350_n1_p121_Brodsky

Referencias:

  • Armitage, P., Berry, G., Advanced analysis of qualitative data (1992) Statistical Method in Medical Research, pp. 433-476. , Doyma Editions, Barcelona
  • Chaplinski, T.J., Niedel, J.E., Cyclic nucleotide-induced maturation of human promyelocytic leukemia cells (1982) J. Clin. Invest., 70, pp. 953-960
  • Cho-Chung, Y.S., Clair, T., Tortora, G., Yokozaki, H., Role of site-selective cAMP analogs in the control and reversal of malignancy (1991) Pharmacol. Ther., 50, pp. 1-33
  • Collins, S.J., Ruscetti, F.W., Gallagher, R.E., Gallo, R.C., Terminal differentiation of human promyelocytic leukemia cells induced by dimethylsulfoxide and other polar compounds (1978) Proc. Natl. Acad. Sci. USA, 75, pp. 2458-2462
  • Daly, J.W., Forskolin, adenylate cyclase and cell physiology: An overview (1984) Adv. Cyclic Nucleotide Protein Phosphorylation Res., 17, pp. 81-89
  • Davio, C., Baldi, A., Shayo, C., Brodsky, A., Cricco, G., Bergoc, R., Rivera, E., H 1 and H 2 histamine receptors in histiocytic lymphoma cell line U937 (1995) Inflamm. Res., 44 (S1), pp. 72-73
  • Davio, C., Cricco, G., Bergoc, R., Rivera, E., H 1 and H 2 histamine receptors in N-nitroso-N-methylurea (NMU)-induced carcinomas with atypical coupling to signal transducers (1995) Biochem. Pharmacol., 50, pp. 91-96
  • Duprez, E., Gjertsen, B.T., Bernard, O., Lanotte, M., Døkesland, S.O., Antiapoptotic effect of heterozygously expressed mutant RI (Ala 336-Asp) subunit of cAMP kinase I in a rat leukemic cell line (1993) J. Biol. Chem., 268, pp. 8332-8336
  • Fischkoff, S.A., Condon, M.E., Switch in differentiative response to maturation inducers of human promyelocytic leukemia cells by prior exposure to alkaline conditions (1985) Cancer Res., 45, pp. 2065-2072
  • Gavison, R., Matzner, Y., Fibach, E., Differential induction of monocytic functions by dibutyryl cyclic AMP and retinoic acid in a human monoblast cell line U937 (1988) Isr. J. Med. Sci., 24, pp. 697-701
  • Hansen, M.B., Nielsen, S.E., Berg, K., Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill (1989) J. Immunol. Methods, 119, pp. 203-210
  • Hartwell, L., CAMPing out (1994) Science, 371, pp. 286-288
  • Hewison, M., Brennan, A., Singh-Ranger, R., Walters, J.C., Katz, D.R., O'Riordan, J.L., The comparative role of 1,25-dihydroxycholecalciferol and phorbol esters in the differentiation of the U937 cell line (1992) Immunology, 77, pp. 304-311
  • Hutt-Taylor, S.R., Harnish, D., Richardson, M., Ishizaka, T., Demburg, J.A., Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60 (1988) Blood, 71, pp. 209-215
  • Kaliniyak, K.A., Sawutz, D.G., Lampkin, B.C., Johnson, C.L., Whitsett, J.A., Effects of dimaprit on growth and differentiation of human promyelocytic cell line, HL-60 (1985) Life Sci., 36, pp. 1909-1916
  • Laskin, D.L., Beavis, A.J., Sirak, A.A., O'Connell, S.M., Laskin, J.D., Differentiation of U937 histiocytic lymphoma cells towards mature neutrophilic granulocytes by dibutyryl cyclic adenosine-3′,5′-monophosphate (1990) Cancer Res., 50, pp. 20-25
  • McConkey, D.J., Orrenius, S., Jondal, M., Agents that elevate cAMP stimulate DNA fragmentation in thymocytes (1990) J. Immunol., 145, pp. 1227-1233
  • Nakamura, K., Takahashi, T., Sasaki, Y., Tsuyuoka, R., Okuno, Y., Kurino, M., Ohmori, K., Nakao, K., 1,25-dihydroxyvitamin D 3 differentiates normal neutrophilic promyelocytes to monocytes/macrophages in vitro (1996) Blood, 87, pp. 2693-2701
  • Nambu, M., Morita, M., Watanabe, H., Venoyama, Y., King, K.M., Takana, M., Iwai, Y., Mikawa, H., Regulation of Fcγ receptor expression and phagocytosis of a human monoblast cell line U937 (1989) J. Immunol., 143, pp. 4158-4165
  • Nilsson, K., Andersson, L.C., Gammberg, C.G., Forsbeck, K., Induction of differentiation in vitro of human leukaemia and lymphoma cell lines (1980) International Symposium on New Trends in Human Immunology and Cancer Immunotherapy, pp. 271-282. , Serrou, B., Rosenfeld, C. (Eds.). Doin Editeurs, Paris
  • Nonaka, T., Mio, M., Doi, M., Tasaka, K., Histamine-induced differentiation of HL-60 cells. The role of cAMP and protein kinase A (1992) Biochem. Pharmacol., 44, pp. 1115-1121
  • Ôberg, F., Botling, J., Nilsson, K., Functional antagonism between vitamin D 3 and retinoic acid in the regulation of CD14 and CD23 expression during monocytic differentiation of U-937 cells (1993) J. Immunol., 150, pp. 3487-3495
  • Olsson, I.L., Breitman, T.R., Induction of differentiation of the human histiocytic lymphoma cell line U-937 by retinoic acid and cyclic adenosine 3′,5′-monophosphate-inducing agents (1982) Cancer Res., 4, pp. 3924-3927
  • Olsson, Y., Gullberg, U., Ivhed, I., Nilsson, K., Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1,25-dihydroxycholecalciferol (1983) Cancer Res., 43, pp. 5862-5867
  • Posternak, T.H., Sutherland, E.W., Henion, W.F., Derivatives of cyclic 3′,5′-adenosine monophosphate (1962) Biochim. Biophys. Acta, 65, pp. 558-560
  • Rigby, W.F.C., Shen, L., Ball, E.D., Guyre, P.M., Fanger, M.W., Differentiation of a human monocytic cell line by 1,25-dihydroxyvitamin D 3 (calcitriol): A morphologic, phenotypic, and functional analysis (1984) Blood, 64, pp. 1110-1115
  • Rubin, J.E., Chenoweth, D.E., Catherwood, B.D., 1,25-dihydroxyvitamin D 3 and adenosine 3′,5′-monophosphate synergistically promote differentiation of a monocytic cell line (1986) Endocrinology, 118, pp. 2540-2545
  • Seamon, K.B., Padgett, W., Daly, J.W., Forskolin: Unique diterpene activator of adenylate cyclase in membranes and in intact cells (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 3363-3367
  • Shayo, C., Davio, C., Brodsky, A., Mladovan, A.G., Lemos Legnazzi, B., Rivera, E., Baldi, A., Histamine modulates the expression of c-fos through cyclic AMP production via H 2 receptor in the human promonocytic cell line U-937 (1997) Mol. Pharmacol., 51, pp. 983-990
  • Testa, U., Masciulli, R., Tritarelli, E., Pustorino, R., Mariani, G., Martucci, R., Barbieri, T., Peschle, C., Transforming growth factor-β potentiates vitamin D 3 -induced terminal monocytic differentiation of human leukemic cell lines (1993) J. Immunol., 150, pp. 2418-2430
  • Vintermyr, O., Gjertsen, B., Lanotte, M., Døkeland, S., Microinjected catalytic subunit of cAMP dependent protein kinase induces apoptosis in myeloid leukemia (IPC-81) cells (1993) Exp. Cell. Res., 206, pp. 157-163

Citas:

---------- APA ----------
Brodsky, A., Davio, C., Shayo, C., Legnazzi, B.L., Barbosa, M., Lardo, M., Morelli, A.,..., Rivera, E. (1998) . Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation. European Journal of Pharmacology, 350(1), 121-127.
http://dx.doi.org/10.1016/S0014-2999(98)00227-1
---------- CHICAGO ----------
Brodsky, A., Davio, C., Shayo, C., Legnazzi, B.L., Barbosa, M., Lardo, M., et al. "Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation" . European Journal of Pharmacology 350, no. 1 (1998) : 121-127.
http://dx.doi.org/10.1016/S0014-2999(98)00227-1
---------- MLA ----------
Brodsky, A., Davio, C., Shayo, C., Legnazzi, B.L., Barbosa, M., Lardo, M., et al. "Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation" . European Journal of Pharmacology, vol. 350, no. 1, 1998, pp. 121-127.
http://dx.doi.org/10.1016/S0014-2999(98)00227-1
---------- VANCOUVER ----------
Brodsky, A., Davio, C., Shayo, C., Legnazzi, B.L., Barbosa, M., Lardo, M., et al. Forskolin induces U937 cell line differentiation as a result of a sustained cAMP elevation. Eur. J. Pharmacol. 1998;350(1):121-127.
http://dx.doi.org/10.1016/S0014-2999(98)00227-1