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Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 30 min after treatment with all three differentiating compounds and lasted at least 48 h. No alteration in the levels of DNA topoisomerase I transcript or protein was observed during these treatments. The results might be explained by post-translational events that render DNA topoisomerase type I less active.


Documento: Artículo
Título:Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
Autor:Shayo, C.C.; Mladovan, A.G.; Baldi, A.
Filiación:Inst. of Biol. and Exp. Med.-CONICET, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Differentiation; Topoisomerase I; U-937 cell; 4alpha phorbol; dna topoisomerase; hexamethylenebisacetamide; phorbol 13 acetate 12 myristate; retinoic acid; article; cell differentiation; controlled study; enzyme activity; human; human cell; leukemia cell; priority journal; Acetamides; Antineoplastic Agents; Blotting, Northern; Blotting, Western; Carcinogens; Cell Transformation, Neoplastic; DNA Topoisomerases, Type I; Electrophoresis, Polyacrylamide Gel; Humans; Leukemia, Monocytic, Chronic; Tetradecanoylphorbol Acetate; Transcription, Genetic; Tretinoin; Tumor Cells, Cultured
Página de inicio:129
Página de fin:133
Título revista:European Journal of Pharmacology
Título revista abreviado:EUR. J. PHARMACOL.
CAS:Acetamides; Antineoplastic Agents; Carcinogens; DNA Topoisomerases, Type I, EC; hexamethylene bisacetamide, 3073-59-4; Tetradecanoylphorbol Acetate, 16561-29-8; Tretinoin, 302-79-4


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---------- APA ----------
Shayo, C.C., Mladovan, A.G. & Baldi, A. (1997) . Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells. European Journal of Pharmacology, 324(1), 129-133.
---------- CHICAGO ----------
Shayo, C.C., Mladovan, A.G., Baldi, A. "Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells" . European Journal of Pharmacology 324, no. 1 (1997) : 129-133.
---------- MLA ----------
Shayo, C.C., Mladovan, A.G., Baldi, A. "Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells" . European Journal of Pharmacology, vol. 324, no. 1, 1997, pp. 129-133.
---------- VANCOUVER ----------
Shayo, C.C., Mladovan, A.G., Baldi, A. Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells. EUR. J. PHARMACOL. 1997;324(1):129-133.