Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Contractile responses to exogenous sodium arachidonate (NaA) were studied in auricles from normal and acutely diabetic (streptozotocin-treated) rats. NaA induced a concentration-dependent positive inotropic effect in atria from normal and diabetic rats but the magnitude of the contractile influence of the fatty acid was different in both types of auricles, i.e. diabetic atria had a greater resonsiveness to NaA than normal preparations. Blockers of arachidonic acid (AA) metabolism via cyclo-oxygenase (indomethacin and acetylsalicylic acid), abolished the stimulatory influence of NaA in normal and in diabetic auricles. Moreover, incubation with tranylcipromine, a prostacyclin synthetase inhibitor, reduced the inotropic effect of NaA in normal atria, but failed to modify it in the diabetic atria. On the other hand, inhibitors of thromboxane (TX) synthetase (imidazole and L-8027) diminished the effect of NaA in diabetic preparations without altering the contractile responses in the controls. In the presence of lipoxygenase blocking agents (nordihydroguaiaretic acid and dithizone), the influence of NaA was reduced at normal values only in the atria from diabetic rats. Both atrial preparations (normal and diabetic) converted ([1-14C]arachidonic acid (AA) into PGI2 (assessed as 6-oxo-PGF1α) and TXB2. Moreover, normal atria generated more PGI2 than TXB2 whereas TXB2 production was greater than that of PGI2 in diabetic atria. The foregoing results suggest that in non-diabetic atria the effect of exogenous NaA may be attributed to metabolites generated by the sequential action of cyclo-oxygenase and prostacyclin synthetase. On the other hand, the contractile action of exogenous NaA in diabetic atria may be ascribed to thromboxanes and lipoxygenase(s) metabolites. A possible modulatory influence of TX on the action of lipoxygenase products is postulated. © 1985.

Registro:

Documento: Artículo
Título:The positive inotropic effect of sodium arachidonate on auricles from diabetic rats
Autor:Canga, L.; Sterin-Borda, L.; Borda, E.S.; Peredo, H.; Gimeno, A.L.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET), Serrano 665/9, 1414 Buenos Aires, Argentina
Palabras clave:Arachidonic acid; Atria; Cyclo-oxygenase; Diabetes; Lipoxygenase(s); Prostacyclin; Streptozotocin; Thromboxane; acetylsalicylic acid; arachidonic acid; arachidonic acid c 14; dithizone; imidazole; indometacin; lipoxygenase; nictindole; nordihydroguaiaretic acid; prostaglandin synthase; prostaglandin synthase inhibitor; radioisotope; streptozocin; tranylcypromine; unclassified drug; animal cell; animal experiment; animal model; article; diabetes mellitus; drug efficacy; drug interaction; endocrine system; heart; heart atrium; inotropism; nonhuman; priority journal; rat; Animals; Arachidonic Acid; Arachidonic Acids; Carbon Radioisotopes; Cyclooxygenase Inhibitors; Diabetes Mellitus, Experimental; Epoprostenol; Heart Atria; Lipoxygenase Inhibitors; Male; Myocardial Contraction; Rats; Rats, Inbred Strains; Stimulation, Chemical; Thromboxane-A Synthase
Año:1985
Volumen:110
Número:1
Página de inicio:47
Página de fin:54
DOI: http://dx.doi.org/10.1016/0014-2999(85)90027-5
Título revista:European Journal of Pharmacology
Título revista abreviado:Eur. J. Pharmacol.
ISSN:00142999
CODEN:EJPHA
CAS:acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; dithizone, 60-10-6; imidazole, 1467-16-9, 288-32-4; indometacin, 53-86-1, 74252-25-8, 7681-54-1; lipoxygenase, 9027-17-2, 9029-60-1; nictindole, 36504-64-0; nordihydroguaiaretic acid, 500-38-9; prostaglandin synthase, 39391-18-9, 59763-19-8, 9055-65-6; streptozocin, 18883-66-4; tranylcypromine, 13492-01-8, 155-09-9, 54-97-7; Arachidonic Acid, 506-32-1; Arachidonic Acids; Carbon Radioisotopes; Cyclooxygenase Inhibitors; Epoprostenol, 35121-78-9; Lipoxygenase Inhibitors; Thromboxane-A Synthase, 5.3.99.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v110_n1_p47_Canga

Referencias:

  • Armstrong, Lattimer, Moncada, Vane, Comparison of the vasodepressor effects of prostacyclin and 6-oxo-prostaglandin F1α with those of prostaglandin E2 in rats and rabbits (1978) Br. J. Pharmacol., 62, p. 125
  • Borbola, Süsskand, Siess, Szekeres, The effects of arachidonic acid in isolated atria of guinea pigs (1977) European J. Pharmacol., 41, p. 27
  • Borda, De Bracco, Finiasz, Sterin-Borda, Lymphocytes sensitize rat isolated atria to the inotropic and chronotropic effects of sodium arachidonate (1984) Br. J. Pharmacol., 81, p. 75
  • Chen, Robertson, Effects of prostaglandin synthesis inhibitors on human insulin secretion and carbohydrate tolerance (1979) Prostaglandins, 18, p. 557
  • Fein, Kornstein, Strobeck, Capasso, Sonnenblick, Altered myocardial mechanics in diabetic rats (1980) Circ. Res., 47, p. 922
  • Fisher, Yates, (1957) Statistical Tables for Biological, Agricultural and Medical Research, , 5th ed., Hafner Publishing Co, New York
  • Gerrard, Stuart, Rao, Steffes, Mauer, Brown, White, Alteration in the balance of prostaglandin and thrombaxane synthesis in diabetes (1980) J. Lab. Clin. Med., 95, p. 950
  • Gryglewski, Prostaglandins and thromboxane biosynthesis inhibitors (1977) Naunyn-Schmiedebergs Archives of Pharmacology, 297, p. 585
  • Gryglewski, Bunting, Moncada, Flower, Vane, Arterial walls are protected against deposition of platelet thrombi by a substance (prostaglandin X) which they make from prostaglandin endoperoxides (1976) Prostaglandins, 12, p. 685
  • Ham, Egan, Soderman, Gale, Kuehl, Peroxidase-dependent deactivation of prostacyclin-synthetase (1979) J. Biol. Chem., 254, p. 2191
  • Hamberg, On the formation of thromboxane B2 and 12L-hydroxy-5,8,10,14-eicosatetraenoic (12 OH-20:4) in tissues from guinea pig (1976) Biochim. Biophys. Acta, 431, p. 651
  • Hamby, Zoneraich, Sherman, Diabetic cardiomyophaty (1974) J. Am. Med. Assoc., 229, p. 1749
  • Harrison, Reece, Johnson, Decreased vascular prostacyclin in experimental diabetes (1978) Life Sci., 23, p. 351
  • Johnson, Harrison, Platelet abnormalities in experimental diabetes (1979) Thromb. Haemost., 42, p. 333
  • Ledet, Neubauer, Christensen, Lundback, Diabetic cardiopathy (1979) Diabetología, 16, p. 207
  • Metz, Feedback modulation of glucose-induced insulin secretion by arachidonic acid metabolites: possible molecular mechanisms and relevance to diabetes mellitus (1981) Prostagland. Med., 7, p. 581
  • Moncada, Bunting, Mullane, Thorogood, Vane, Raz, Needleman, Imidazole: a selective potent antagonist of thromboxane synthetase (1977) Prostaglandins, 13, p. 611
  • Moncada, Vane, Unstable metabolites of arachidonic acid and their role in haemostatis and thrombosis (1978) Br. Med. Bull., 34, p. 129
  • Penparkful, Schaible, Yipintsoi, Scheuer, The effect of diabetes on performance and metabolism of rat hearts (1980) Circ. Res., 47, p. 911
  • Pistorius, Axelrod, Iron, an essential component of lipoxygenase (1974) J. Biol. Chem., 249, p. 3183
  • Robertson, Prostaglandins as modulators of pancreatic islet function (1979) Diabetes, 28, p. 943
  • Rösen, Schrör, Increased prostacyclin release from perfused hearts of acutely diabetic rats (1980) Diabetologia, 18, p. 391
  • Roth, Reibel, Lefer, Vascular responsiveness and eicosanoid production in diabetic rats (1983) Diabetologia, 24, p. 372
  • Salmon, Smith, Flower, Moncada, Vane, Further studies on the enzymatic conversion of prostaglandin endoperoxide into prostacyclin by porcine aorta microsomes (1978) Biochim. Biophys. Acta, 523, p. 250
  • Schrör, Moncada, Ubatuba, Vane, Transformation of arachidonic acid and prostaglandin endoperoxides by the guinea pig heart Formation of RCS and prostacyclin (1978) European Journal of Pharmacology, 47, p. 103
  • Shapiro, Howat, Calfer, Left ventricular function in diabetes mellitus. I: Methodology, prevalence and spectrum of abnormalities (1981) Br. Heart J., 45, p. 122
  • Shapiro, Leatherdale, Mackinnon, Fletcher, Left ventricular function in diabetes mellitus. II: Relation between clinical features and left ventricular functions (1981) Br. Heart J., 45, p. 129
  • Sterin-Borda, Canga, Borda, Gimeno, Gimeno, Inotropic effect of prostacyclin (PGI2) on isolated atria at different contraction frequencies (1980) Naunyn-Schmiedeb. Arch. Pharmacol., 313, p. 95
  • Sterin-Borda, Canga, Gimeno, Stimulating effect of thromboxane B2 on isolated rat atria (1981) Experientia, 37, p. 592
  • Sterin-Borda, Canga, Gimeno, Gimeno, An adrenergic participation subserving a positive inotropism and chronotropism of prostacyclin on isolated rat atria (1979) Experientia, 35, p. 529
  • Sterin-Borda, Gimeno, Del Castillo, Gimeno, Prostacyclin (PGI2) and U-46619 stimulate coronary arteries from diabetic dogs and their action is influenced by inhibitors of prostaglandin biosynthesis (1981) Prostaglandins, 22, p. 267
  • Vane, Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs (1971) Nature New Biol., 231, p. 232
  • Van Rossum, Cumulative dose-response curves. II: Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters (1963) Arch. Int. Pharmacodyn. Ther., 143, p. 299

Citas:

---------- APA ----------
Canga, L., Sterin-Borda, L., Borda, E.S., Peredo, H. & Gimeno, A.L. (1985) . The positive inotropic effect of sodium arachidonate on auricles from diabetic rats. European Journal of Pharmacology, 110(1), 47-54.
http://dx.doi.org/10.1016/0014-2999(85)90027-5
---------- CHICAGO ----------
Canga, L., Sterin-Borda, L., Borda, E.S., Peredo, H., Gimeno, A.L. "The positive inotropic effect of sodium arachidonate on auricles from diabetic rats" . European Journal of Pharmacology 110, no. 1 (1985) : 47-54.
http://dx.doi.org/10.1016/0014-2999(85)90027-5
---------- MLA ----------
Canga, L., Sterin-Borda, L., Borda, E.S., Peredo, H., Gimeno, A.L. "The positive inotropic effect of sodium arachidonate on auricles from diabetic rats" . European Journal of Pharmacology, vol. 110, no. 1, 1985, pp. 47-54.
http://dx.doi.org/10.1016/0014-2999(85)90027-5
---------- VANCOUVER ----------
Canga, L., Sterin-Borda, L., Borda, E.S., Peredo, H., Gimeno, A.L. The positive inotropic effect of sodium arachidonate on auricles from diabetic rats. Eur. J. Pharmacol. 1985;110(1):47-54.
http://dx.doi.org/10.1016/0014-2999(85)90027-5