Artículo

Ziblat, A.; Domaica, C.I.; Spallanzani, R.G.; Iraolagoitia, X.L.R.; Rossi, L.E.; Avila, D.E.; Torres, N.I.; Fuertes, M.B.; Zwirner, N.W. "IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness" (2015) European Journal of Immunology. 45(1):192-202
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Abstract:

IL-27, a member of the IL-12 family of cytokines, is produced by APCs, and displays pro- and anti-inflammatory effects. How IL-27 affects human NK cells still remains unknown. In this study, we observed that mature DCs secreted IL-27 and that blockade of IL-27R (CD130) reduced the amount of IFN-γ produced by NK cells during their coculture, showing the importance of IL-27 during DC-NK-cell crosstalk. Accordingly, human rIL-27 stimulated IFN-γ secretion by NK cells in a STAT1-dependent manner, induced upregulation of CD25 and CD69 on NK cells, and displayed a synergistic effect with IL-18. Preincubation experiments demonstrated that IL-27 primed NK cells for IL-18-induced IFN-γ secretion, which was associated with an IL-27-driven upregulation of T-bet expression. Also, IL-27 triggered NKp46-dependent NK-cell-mediated cytotoxicity against Raji, T-47D, and HCT116 cells, and IL-18 enhanced this cytotoxic response. Such NK-cell-mediated cytotoxicity involved upregulation of perforin, granule exocytosis, and TRAIL-mediated cytotoxicity but not Fas-FasL interaction. Moreover, IL-27 also potentiated Ab-dependent cell-mediated cytotoxicity against mAb-coated target cells. Taken together, IL-27 stimulates NK-cell effector functions, which might be relevant in different physiological and pathological situations. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Registro:

Documento: Artículo
Título:IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness
Autor:Ziblat, A.; Domaica, C.I.; Spallanzani, R.G.; Iraolagoitia, X.L.R.; Rossi, L.E.; Avila, D.E.; Torres, N.I.; Fuertes, M.B.; Zwirner, N.W.
Filiación:Laboratorio de Fisiopatología de la Inmunidad Innata, Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Cátedra de Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Cytotoxicity; IFN-γ; IL-18; IL-27; NK cells; CD69 antigen; Fas antigen; Fas ligand; fludarabine; gamma interferon; glycoprotein gp 130; immunoglobulin enhancer binding protein; interleukin 10; interleukin 12; interleukin 15; interleukin 17; interleukin 18; interleukin 2; interleukin 2 receptor alpha; interleukin 27; interleukin 4; interleukin 6; Janus kinase 2; mammalian target of rapamycin; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen activated protein kinase p38; natural cytotoxicity triggering receptor 1; perforin; phosphatidylinositol 3 kinase; STAT1 protein; stress activated protein kinase; transcription factor T bet; tumor necrosis factor; tumor necrosis factor related apoptosis inducing ligand; CD69 antigen; glycoprotein gp 130; IL27 protein, human; IL2RA protein, human; IL6ST protein, human; interleukin 18; interleukin 18 protein, human; interleukin 2 receptor alpha; interleukin derivative; lectin; leukocyte antigen; monoclonal antibody; recombinant protein; T lymphocyte antigen; Article; cancer cell line; cell maturation; cell mediated cytotoxicity; cell surface; cell viability; coculture; controlled study; cytokine release; cytokine response; dendritic cell; effector cell; enzyme linked immunosorbent assay; exocytosis; flow cytometry; HCT116 cell line; human; human cell; lymphocyte function; natural killer cell; priority journal; protein expression; protein protein interaction; Raji cell line; secretory pathway; T 47D cell; target cell; upregulation; cell proliferation; cell survival; cytology; cytotoxicity; drug effects; gene expression regulation; genetics; immunology; natural killer cell; primary cell culture; signal transduction; Antibodies, Monoclonal; Antigens, CD; Antigens, Differentiation, T-Lymphocyte; Cell Proliferation; Cell Survival; Coculture Techniques; Cytokine Receptor gp130; Cytotoxicity, Immunologic; Dendritic Cells; Gene Expression Regulation; HCT116 Cells; Humans; Interleukin-18; Interleukin-2 Receptor alpha Subunit; Interleukins; Killer Cells, Natural; Lectins, C-Type; Primary Cell Culture; Recombinant Proteins; Signal Transduction
Año:2015
Volumen:45
Número:1
Página de inicio:192
Página de fin:202
DOI: http://dx.doi.org/10.1002/eji.201444699
Título revista:European Journal of Immunology
Título revista abreviado:Eur. J. Immunol.
ISSN:00142980
CODEN:EJIMA
CAS:fludarabine, 21679-14-1; gamma interferon, 82115-62-6; interleukin 12, 138415-13-1; interleukin 18, 189304-55-0; interleukin 2, 85898-30-2; perforin, 119332-27-3; phosphatidylinositol 3 kinase, 115926-52-8; stress activated protein kinase, 155215-87-5; Antibodies, Monoclonal; Antigens, CD; Antigens, Differentiation, T-Lymphocyte; CD69 antigen; Cytokine Receptor gp130; IL27 protein, human; IL2RA protein, human; IL6ST protein, human; interleukin 18 protein, human; Interleukin-18; Interleukin-2 Receptor alpha Subunit; Interleukins; Lectins, C-Type; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142980_v45_n1_p192_Ziblat

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Citas:

---------- APA ----------
Ziblat, A., Domaica, C.I., Spallanzani, R.G., Iraolagoitia, X.L.R., Rossi, L.E., Avila, D.E., Torres, N.I.,..., Zwirner, N.W. (2015) . IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. European Journal of Immunology, 45(1), 192-202.
http://dx.doi.org/10.1002/eji.201444699
---------- CHICAGO ----------
Ziblat, A., Domaica, C.I., Spallanzani, R.G., Iraolagoitia, X.L.R., Rossi, L.E., Avila, D.E., et al. "IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness" . European Journal of Immunology 45, no. 1 (2015) : 192-202.
http://dx.doi.org/10.1002/eji.201444699
---------- MLA ----------
Ziblat, A., Domaica, C.I., Spallanzani, R.G., Iraolagoitia, X.L.R., Rossi, L.E., Avila, D.E., et al. "IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness" . European Journal of Immunology, vol. 45, no. 1, 2015, pp. 192-202.
http://dx.doi.org/10.1002/eji.201444699
---------- VANCOUVER ----------
Ziblat, A., Domaica, C.I., Spallanzani, R.G., Iraolagoitia, X.L.R., Rossi, L.E., Avila, D.E., et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur. J. Immunol. 2015;45(1):192-202.
http://dx.doi.org/10.1002/eji.201444699