Artículo
Tomio, J.M.; Tuzman, V.; Grinstein, M. "δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties" (1968) European Journal of Biochemistry. 6(1):84-87
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Abstract:
δ‐Aminolevulinate dehydratase which catalyzes the second step in the biosynthesis of protoporphyrin has been purified from Harderian gland homogenates of normal rats. The enzyme preparation has a specific activity of 860 units per mg of protein at 37°, and about a 150‐fold purification from the crude extract has been achieved. The purified material appears to be homogeneous in starch gel and polyacrylamide gel disc electrophoretic studies. Partially purified enzyme preparations have an absolute requirement for cysteine or analogous thiol reagents. Effect of anions and cations is discussed. Copyright © 1968, Wiley Blackwell. All rights reserved
Registro:
| Documento: | Artículo |
| Título: | δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties |
| Autor: | Tomio, J.M.; Tuzman, V.; Grinstein, M. |
| Filiación: | Departamento de Química Biológica, Laboratorio de Química Biológica I, Facultad de Ciencias Exactas y Naturales, Perú 272, Buenos Aires, Argentina |
| Palabras clave: | amino acid; chelating agent; chloride; cysteine; edetic acid; electrolyte; Hydro Lyases; hydrolyase; levulinic acid; porphyrin; sulfate; thiol derivative; animal; article; chemistry; electrophoresis; enzymology; gel chromatography; gel electrophoresis; isolation and purification; lacrimal apparatus; nictitating membrane; precipitation; rat; Amino Acids; Animal; Chelating Agents; Chemistry; Chlorides; Chromatography, Gel; Cysteine; Edetic Acid; Electrolytes; Electrophoresis; Electrophoresis, Disc; Hydro-Lyases; Lacrimal Apparatus; Levulinic Acids; Nictitating Membrane; Porphyrins; Precipitation; Rats; Sulfates; Sulfhydryl Compounds |
| Año: | 1968 |
| Volumen: | 6 |
| Número: | 1 |
| Página de inicio: | 84 |
| Página de fin: | 87 |
| DOI: | http://dx.doi.org/10.1111/j.1432-1033.1968.tb00422.x |
| Título revista: | European Journal of Biochemistry |
| Título revista abreviado: | Eur. J. Biochem. |
| ISSN: | 00142956 |
| CAS: | amino acid, 65072-01-7; chloride, 16887-00-6; cysteine, 4371-52-2, 52-89-1, 52-90-4; edetic acid, 150-43-6, 60-00-4; hydrolyase, 9044-86-4; levulinic acid, 123-76-2; porphyrin, 24869-67-8; sulfate, 14808-79-8; thiol derivative, 13940-21-1; Amino Acids; Chelating Agents; Chlorides; Cysteine, 52-90-4; Edetic Acid, 60-00-4; Electrolytes; Hydro-Lyases, EC 4.2.1.; Levulinic Acids; Porphyrins; Sulfates; Sulfhydryl Compounds |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v6_n1_p84_Tomio |
Citas:
---------- APA ----------
Tomio, J.M., Tuzman, V. & Grinstein, M. (1968) . δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties. European Journal of Biochemistry, 6(1), 84-87.http://dx.doi.org/10.1111/j.1432-1033.1968.tb00422.x
---------- CHICAGO ----------
Tomio, J.M., Tuzman, V., Grinstein, M. "δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties" . European Journal of Biochemistry 6, no. 1 (1968) : 84-87.http://dx.doi.org/10.1111/j.1432-1033.1968.tb00422.x
---------- MLA ----------
Tomio, J.M., Tuzman, V., Grinstein, M. "δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties" . European Journal of Biochemistry, vol. 6, no. 1, 1968, pp. 84-87.http://dx.doi.org/10.1111/j.1432-1033.1968.tb00422.x
---------- VANCOUVER ----------
Tomio, J.M., Tuzman, V., Grinstein, M. δ‐Aminolevulinate Dehydratase from Rat Harderian Gland Purification and Properties. Eur. J. Biochem. 1968;6(1):84-87.http://dx.doi.org/10.1111/j.1432-1033.1968.tb00422.x
