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Abstract:

The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
Autor:GarcÍA, R.C.; Recondo, E.; Dankert, M.
Filiación:Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Obligado, Buenos Sires, 2490, Argentina
Departamento de Química Biologica, Facultad de Ciencas Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:lipid; magnesium; manganese; polysaccharide; uridine diphosphate glucose; acetobacter xylinum; in vitro study; microorganism; theoretical study; Acetobacter; Animal; Borohydrides; Butanols; Carbon Radioisotopes; Cell Division; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Chromatography, Paper; Electrophoresis, Paper; Galactose; Glucose; Hexokinase; Kinetics; Lipids; Magnesium; Manganese; Muscles; Phosphoglucomutase; Phosphorus Radioisotopes; Polysaccharides, Bacterial; Rabbits; Saccharomyces cerevisiae; Solubility; Time Factors; Uridine Diphosphate Sugars
Año:1974
Volumen:43
Número:1
Página de inicio:93
Página de fin:105
DOI: http://dx.doi.org/10.1111/j.1432-1033.1974.tb03389.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:lipid, 66455-18-3; magnesium, 7439-95-4; manganese, 16397-91-4, 7439-96-5; uridine diphosphate glucose, 133-89-1; Borohydrides; Butanols; Carbon Radioisotopes; Galactose, 26566-61-0; Glucose, 50-99-7; Hexokinase, EC 2.7.1.1; Lipids; Magnesium, 7439-95-4; Manganese, 7439-96-5; Phosphoglucomutase, EC 5.4.2.2; Phosphorus Radioisotopes; Polysaccharides, Bacterial; Uridine Diphosphate Sugars
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v43_n1_p93_Garcia

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Citas:

---------- APA ----------
GarcÍA, R.C., Recondo, E. & Dankert, M. (1974) . Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars. European Journal of Biochemistry, 43(1), 93-105.
http://dx.doi.org/10.1111/j.1432-1033.1974.tb03389.x
---------- CHICAGO ----------
GarcÍA, R.C., Recondo, E., Dankert, M. "Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars" . European Journal of Biochemistry 43, no. 1 (1974) : 93-105.
http://dx.doi.org/10.1111/j.1432-1033.1974.tb03389.x
---------- MLA ----------
GarcÍA, R.C., Recondo, E., Dankert, M. "Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars" . European Journal of Biochemistry, vol. 43, no. 1, 1974, pp. 93-105.
http://dx.doi.org/10.1111/j.1432-1033.1974.tb03389.x
---------- VANCOUVER ----------
GarcÍA, R.C., Recondo, E., Dankert, M. Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars. Eur. J. Biochem. 1974;43(1):93-105.
http://dx.doi.org/10.1111/j.1432-1033.1974.tb03389.x