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Abstract:

The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
Autor:De Lederkremer, R.M.; Lima, C.E.; Ramirez, M.I.; Goncalvez, M.F.; Colli, W.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de Sño Paulo, Brazil
Palabras clave:ceramide; glycoinositolphospholipid; glycophospoinositol; phospholipase c; unclassified drug; article; epimastigote; nonhuman; priority journal; trypanosoma cruzi; Animal; Carbohydrate Sequence; Ceramides; Chromatography, Gas; Glyceryl Ethers; Glycosylation; Glycosylphosphatidylinositols; Lipids; Methylation; Molecular Sequence Data; Palmitates; Polysaccharides; Spectrum Analysis, Mass; Support, Non-U.S. Gov't; Trypanosoma cruzi; Bacillus thuringiensis; Trypanosoma; Trypanosoma cruzi
Año:1993
Volumen:218
Número:3
Página de inicio:929
Página de fin:936
DOI: http://dx.doi.org/10.1111/j.1432-1033.1993.tb18449.x
Handle:http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:1-O-hexadecyl-2-O-palmitoylglycerol, 17752-41-9; Ceramides; Glyceryl Ethers; Glycosylphosphatidylinositols; Lipids; Palmitates; Polysaccharides
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00142956_v218_n3_p929_DeLederkremer.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v218_n3_p929_DeLederkremer

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Citas:

---------- APA ----------
De Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Goncalvez, M.F. & Colli, W. (1993) . Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi. European Journal of Biochemistry, 218(3), 929-936.
http://dx.doi.org/10.1111/j.1432-1033.1993.tb18449.x
---------- CHICAGO ----------
De Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Goncalvez, M.F., Colli, W. "Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi" . European Journal of Biochemistry 218, no. 3 (1993) : 929-936.
http://dx.doi.org/10.1111/j.1432-1033.1993.tb18449.x
---------- MLA ----------
De Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Goncalvez, M.F., Colli, W. "Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi" . European Journal of Biochemistry, vol. 218, no. 3, 1993, pp. 929-936.
http://dx.doi.org/10.1111/j.1432-1033.1993.tb18449.x
---------- VANCOUVER ----------
De Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Goncalvez, M.F., Colli, W. Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi. Eur. J. Biochem. 1993;218(3):929-936.
http://dx.doi.org/10.1111/j.1432-1033.1993.tb18449.x