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Abstract:

The lipopeptidophosphoglycan (LPPG) from Trypanosoma cruzi, a major constituent of the plasma membrane of epimastigote forms, has been now extracted with butanol/water from delipidated cells and purified by hydrophobic chromatography. We have found that the LPPG undergoes two reactions, characteristic of the glycosylphosphatidylinositol anchors: (a) cleavage of the ceramide by phosphatidylinositol‐specific phospholipase C(PtdIns‐specific phospholipase C) from Bacillus thuringiensis, (b) nitrous acid deamination of the non‐N‐acylated glucosamine. Palmitoylsphinganine, palmitoylsphingosine, lignoceroylsphinganine and, as minor components, the stearoylceramides were identified by gas liquid chromatography/mass spectrometry. The presence of cross reacting determinant (CRD) epitopes in the glycophosphoinositol released by PtdIns‐specific phospholipase C was investigated by direct and inhibition ELISA. A sample of glycophosphoinositol containing 5 μg carbohydrate caused 60% inhibition of the binding of anti‐CRD antibodies raised against the soluble form of variant surface glycoprotein. Copyright © 1990, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
Autor:de LEDERKREMER, R.M.; LIMA, C.; RAMIREZ, M.I.; CASAL, O.L.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:glycan; lipid; peptide; phospholipase; article; cell membrane; enzyme linked immunosorbent assay; invertebrate; nonhuman; priority journal; protozoon; trypanosoma cruzi; Animal; Ceramides; Chromatography, High Pressure Liquid; Enzyme-Linked Immunosorbent Assay; Glycolipids; Glycosylphosphatidylinositols; Oxidation-Reduction; Peptidoglycan; Phosphatidylinositols; Phospholipase C; Phospholipids; Support, Non-U.S. Gov't; Trypanosoma cruzi; Bacillus thuringiensis; Invertebrata; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:1990
Volumen:192
Número:2
Página de inicio:337
Página de fin:345
DOI: http://dx.doi.org/10.1111/j.1432-1033.1990.tb19232.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:Ceramides; Glycolipids; Glycosylphosphatidylinositols; lipopeptidophosphoglycan; Peptidoglycan; Phosphatidylinositols; Phospholipase C, EC 3.1.4.3; Phospholipids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v192_n2_p337_deLEDERKREMER

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Citas:

---------- APA ----------
de LEDERKREMER, R.M., LIMA, C., RAMIREZ, M.I. & CASAL, O.L. (1990) . Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors. European Journal of Biochemistry, 192(2), 337-345.
http://dx.doi.org/10.1111/j.1432-1033.1990.tb19232.x
---------- CHICAGO ----------
de LEDERKREMER, R.M., LIMA, C., RAMIREZ, M.I., CASAL, O.L. "Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors" . European Journal of Biochemistry 192, no. 2 (1990) : 337-345.
http://dx.doi.org/10.1111/j.1432-1033.1990.tb19232.x
---------- MLA ----------
de LEDERKREMER, R.M., LIMA, C., RAMIREZ, M.I., CASAL, O.L. "Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors" . European Journal of Biochemistry, vol. 192, no. 2, 1990, pp. 337-345.
http://dx.doi.org/10.1111/j.1432-1033.1990.tb19232.x
---------- VANCOUVER ----------
de LEDERKREMER, R.M., LIMA, C., RAMIREZ, M.I., CASAL, O.L. Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors. Eur. J. Biochem. 1990;192(2):337-345.
http://dx.doi.org/10.1111/j.1432-1033.1990.tb19232.x