Abstract:
Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N‐ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin‐depleted retinal membranes were not capable of reconstituting the heterologous light‐stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N‐ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system. Copyright © 1989, Wiley Blackwell. All rights reserved
Registro:
Documento: |
Artículo
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Título: | Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas |
Autor: | Muschietti, J.P.; Bianchini, G.M.; Martinetto, H.E.; Carricarte, V.C.; Giusto, N.; Farber, D.B.; Torres, H.N.; Flawia, M.M. |
Filiación: | Instituto de Investigaciones En Ingenieria Genética Y Biología Molecular, Facultad de Ciencias Exactas Y Naturales, Buenos Aires, Argentina Jules Stein Eye Institute, University of California School of Medicine, Los Angeles, United States Instituto de Investigaciones Bioquímicas de Bahía Blanca
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Palabras clave: | adenylate cyclase; radioisotope; animal cell; cattle; fungus; light; membrane; neurospora crassa; nonhuman; priority journal; retina; Adenosine Diphosphate Ribose; Adenylate Cyclase; Animal; Cattle; Cyclic GMP; Light; Mice; Mice, Inbred C57BL; Neurospora; Neurospora crassa; Retina |
Año: | 1989
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Volumen: | 185
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Número: | 1
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Página de inicio: | 205
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Página de fin: | 210
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DOI: |
http://dx.doi.org/10.1111/j.1432-1033.1989.tb15103.x |
Título revista: | European Journal of Biochemistry
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Título revista abreviado: | Eur. J. Biochem.
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ISSN: | 00142956
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CAS: | adenylate cyclase, 9012-42-4; Adenosine Diphosphate Ribose, 20762-30-5; Adenylate Cyclase, EC 4.6.1.1; Cyclic GMP, 7665-99-8
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v185_n1_p205_Muschietti |
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Citas:
---------- APA ----------
Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D.B., Torres, H.N.,..., Flawia, M.M.
(1989)
. Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas. European Journal of Biochemistry, 185(1), 205-210.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb15103.x---------- CHICAGO ----------
Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D.B., et al.
"Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas"
. European Journal of Biochemistry 185, no. 1
(1989) : 205-210.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb15103.x---------- MLA ----------
Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D.B., et al.
"Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas"
. European Journal of Biochemistry, vol. 185, no. 1, 1989, pp. 205-210.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb15103.x---------- VANCOUVER ----------
Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D.B., et al. Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas. Eur. J. Biochem. 1989;185(1):205-210.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb15103.x