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Abstract:

The kinetic properties of the enzyme l‐glutamate:4,5‐dioxovaleric acid aminotransferase (Glu: DOVA transaminase) from Euglena gracilis have been studied. 5‐Aminolevulinic acid formation was linear with time for at least 45 min at 37°C and l‐glutamate was the most effective amino‐group donor. Lineweaver‐Burk double‐reciprocal plots suggested a ping‐pong reaction mechanism, with Km values for l‐glutamate and DOVA of 1.92 mM and 0.48 mM respectively. Competitive parabolic substrate inhibition by DOVA at concentrations greater than 3.5–4.5 mM was observed. Glyoxylate (4–10 mM) was found to be a competitive inhibitor with respect to DOVA, whereas at low concentrations (0–4 mM) noncompetitive plots were obtained. An analysis of the possible enzyme forms involved, was carried out. In more crude preparations most of the enzyme is found to be in the form of an enzyme‐glutamate complex. Copyright © 1989, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Glutamate:4,5‐dioxovaleric acid transaminase from Euglena gracilis Kinetic studies
Autor:LOMBARDO, M.E.; ARAUJO, L.S.; JUKNAT, A.A.; BATLLE, A.M.d.C.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias, Faculdad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:aminolevulinate aminotransferase; aminotransferase; animal; article; darkness; enzyme specificity; enzyme stability; enzymology; Euglena gracilis; growth, development and aging; isolation and purification; kinetics; Animal; Darkness; Enzyme Stability; Euglena gracilis; Kinetics; Substrate Specificity; Support, Non-U.S. Gov't; Transaminases
Año:1989
Volumen:182
Número:3
Página de inicio:657
Página de fin:660
DOI: http://dx.doi.org/10.1111/j.1432-1033.1989.tb14875.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:aminolevulinate aminotransferase, 9012-46-8; aminotransferase, 9031-66-7; aminolevulinate aminotransferase, EC 2.6.1.43; Transaminases, EC 2.6.1.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v182_n3_p657_LOMBARDO

Referencias:

  • Shemin, D., Russell, C.S., (1953) J. Am. Chem. Soc., 75, pp. 4873-4874
  • Beale, S., δ-Aminolevulinic Acid in Plants: Its Biosynthesis, Regulation, and Role in Plastid Development (1978) Annual Review of Plant Physiology, 29, pp. 95-120
  • Klein, O., Dörnemann, D., Senger, H., (1980) Int. J. Biochem., 12, pp. 725-728
  • Weinstein, J.D., Beale, S.I., (1985) Arch. Biochem. Biophys., 237, pp. 454-464
  • Gómez‐Silva, B., Timko, M.P., Schiff, J.A., (1985) Planta, 165, pp. 12-22
  • Klein, O., Senger, U., BIOSYNTHETIC PATHWAYS TO 6-AMINOLEVULINIC ACID INDUCED BY BLUE LIGHT IN THE PIGMENT MUTANT C-2A‘ OF SCENEDESMUS OBLIQUUS (1978) Photochemistry and Photobiology, 27, pp. 203-208
  • Salvador, G.F., (1978) Plant Sci. Lett., 13, pp. 351-355
  • Lombardo, M.E., Araujo, L.S., Juknat, A.A., Batlle, (1989) Comp. Biochem. Physiol., 91 B, pp. 279-284
  • Cleland, W.W., (1970) The enzymes, 2, pp. 1-65. , 3rd edn, Academic Press, New York
  • Noguchi, T., Mori, R., (1981) J. Biol. Chem., 256, pp. 10335-10339
  • Foley, T., Beale, S.L.,  -Aminolevulinic Acid Formation from  , -Dioxovaleric Acid in Extracts of Euglena gracilis (1982) PLANT PHYSIOLOGY, 70, pp. 1495-1502
  • Varticovski, L., Kushner, J.P., Burnham, B.F., (1980) J. Biol. Chem., 255, pp. 3742-3747
  • Gassman, M.L., Pluscec, J., Bogorad, L.,  -Aminolevulinic Acid Transaminase in Chlorella vulgaris (1968) PLANT PHYSIOLOGY, 43, pp. 1411-1414
  • Shioi, Y., Doi, M., Sasa, T., (1984) Plant Cell Physiol., 25, pp. 1487-1493
  • Meisch, H.‐U., Hoffmann, H., Reinle, W., (1983) Biochim. Biophys. Acta, 743, pp. 281-289

Citas:

---------- APA ----------
LOMBARDO, M.E., ARAUJO, L.S., JUKNAT, A.A. & BATLLE, A.M.d.C. (1989) . Glutamate:4,5‐dioxovaleric acid transaminase from Euglena gracilis Kinetic studies. European Journal of Biochemistry, 182(3), 657-660.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb14875.x
---------- CHICAGO ----------
LOMBARDO, M.E., ARAUJO, L.S., JUKNAT, A.A., BATLLE, A.M.d.C. "Glutamate:4,5‐dioxovaleric acid transaminase from Euglena gracilis Kinetic studies" . European Journal of Biochemistry 182, no. 3 (1989) : 657-660.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb14875.x
---------- MLA ----------
LOMBARDO, M.E., ARAUJO, L.S., JUKNAT, A.A., BATLLE, A.M.d.C. "Glutamate:4,5‐dioxovaleric acid transaminase from Euglena gracilis Kinetic studies" . European Journal of Biochemistry, vol. 182, no. 3, 1989, pp. 657-660.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb14875.x
---------- VANCOUVER ----------
LOMBARDO, M.E., ARAUJO, L.S., JUKNAT, A.A., BATLLE, A.M.d.C. Glutamate:4,5‐dioxovaleric acid transaminase from Euglena gracilis Kinetic studies. Eur. J. Biochem. 1989;182(3):657-660.
http://dx.doi.org/10.1111/j.1432-1033.1989.tb14875.x