Artículo
CÁCERES, J.F.; BLANGY, D.; GLIKIN, G.C. "Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts" (1989) European Journal of Biochemistry. 181(2):531-537
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Abstract:
Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved
Registro:
| Documento: | Artículo |
| Título: | Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| Autor: | CÁCERES, J.F.; BLANGY, D.; GLIKIN, G.C. |
| Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Institut de Recherches Scientifiques, Cancer du Centre National de la Recherche Scientifique, Villejuif, France |
| Palabras clave: | DNA; DNA topoisomerase; DNA topoisomerase (ATP hydrolysing); animal; article; cell culture; chromatin; DNA supercoiling; isolation and purification; kinetics; metabolism; mouse; Animal; Cells, Cultured; Chromatin; DNA; DNA Topoisomerases, Type I; DNA Topoisomerases, Type II; DNA, Superhelical; Kinetics; Mice; Support, Non-U.S. Gov't |
| Año: | 1989 |
| Volumen: | 181 |
| Número: | 2 |
| Página de inicio: | 531 |
| Página de fin: | 537 |
| DOI: | http://dx.doi.org/10.1111/j.1432-1033.1989.tb14756.x |
| Título revista: | European Journal of Biochemistry |
| Título revista abreviado: | Eur. J. Biochem. |
| ISSN: | 00142956 |
| CAS: | DNA topoisomerase, 80449-01-0; DNA, 9007-49-2; Chromatin; DNA Topoisomerases, Type I, EC 5.99.1.2; DNA Topoisomerases, Type II, EC 5.99.1.3; DNA, 9007-49-2; DNA, Superhelical |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v181_n2_p531_CACERES |
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Citas:
---------- APA ----------
CÁCERES, J.F., BLANGY, D. & GLIKIN, G.C. (1989) . Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts. European Journal of Biochemistry, 181(2), 531-537.http://dx.doi.org/10.1111/j.1432-1033.1989.tb14756.x
---------- CHICAGO ----------
CÁCERES, J.F., BLANGY, D., GLIKIN, G.C. "Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts" . European Journal of Biochemistry 181, no. 2 (1989) : 531-537.http://dx.doi.org/10.1111/j.1432-1033.1989.tb14756.x
---------- MLA ----------
CÁCERES, J.F., BLANGY, D., GLIKIN, G.C. "Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts" . European Journal of Biochemistry, vol. 181, no. 2, 1989, pp. 531-537.http://dx.doi.org/10.1111/j.1432-1033.1989.tb14756.x
---------- VANCOUVER ----------
CÁCERES, J.F., BLANGY, D., GLIKIN, G.C. Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts. Eur. J. Biochem. 1989;181(2):531-537.http://dx.doi.org/10.1111/j.1432-1033.1989.tb14756.x
