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Abstract:

Two types of linkages between the carbohydrate and the peptide moiety in the glycopeptide from Ascobolus furfuraceus are described. Treatment with mild alkali produced β‐elimination of a small oligosaccharide. Evidence for the O‐glycosidic linkage was provided by (a) increase in absorbance at 240 nm, (b) decrease in threonine and serine content after the alkaline treatment and (c) detection of tritiated oligosaccharide following alkaline NaB3H4 reduction. Mannose is the sugar involved in the O‐glycosidic linkage. The remaining glycopeptide was branched by galactofuranose units, which were selectivity released by mild acid hydrolysis. The N‐glycosidic linkage of the sugar chain was conclusively proved by cleavage with endo‐β‐N‐acetyl‐glucosaminidase. Sequential NaB3H4 reduction and acid hydrolysis gave [3H]glucosaminitol. The structure of the sugar chain was studied by 13C NMR spectroscopy and by methylation analysis. Copyright © 1987, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:d‐Galactofuranose in the N‐linked sugar chain of a glycopeptide from Ascobolus furfuraceus
Autor:GROISMAN, J.F.; de LEDERKREMER, R.M.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:acetylglucosaminidase; amino acid; galactose; glycopeptide; Mannosyl Glycoprotein Endo beta N Acetylglucosaminidase; n acetyl beta glucosaminidase; article; Ascomycetes; gel chromatography; methylation; nuclear magnetic resonance spectroscopy; optical rotation; oxidation reduction reaction; paper chromatography; pH; Acetylglucosaminidase; Amino Acids; Ascomycota; Chromatography, Gel; Chromatography, Paper; Galactose; Glycopeptides; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; Methylation; Optical Rotation; Oxidation-Reduction; Support, Non-U.S. Gov't
Año:1987
Volumen:165
Número:2
Página de inicio:327
Página de fin:332
DOI: http://dx.doi.org/10.1111/j.1432-1033.1987.tb11445.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:acetylglucosaminidase, 9027-56-9; amino acid, 65072-01-7; galactose, 26566-61-0, 50855-33-9, 59-23-4; n acetyl beta glucosaminidase, 37278-88-9; Acetylglucosaminidase, EC 3.2.1.30; Amino Acids; Galactose, 26566-61-0; Glycopeptides; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, EC 3.2.1.96
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v165_n2_p327_GROISMAN

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Citas:

---------- APA ----------
GROISMAN, J.F. & de LEDERKREMER, R.M. (1987) . d‐Galactofuranose in the N‐linked sugar chain of a glycopeptide from Ascobolus furfuraceus. European Journal of Biochemistry, 165(2), 327-332.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb11445.x
---------- CHICAGO ----------
GROISMAN, J.F., de LEDERKREMER, R.M. "d‐Galactofuranose in the N‐linked sugar chain of a glycopeptide from Ascobolus furfuraceus" . European Journal of Biochemistry 165, no. 2 (1987) : 327-332.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb11445.x
---------- MLA ----------
GROISMAN, J.F., de LEDERKREMER, R.M. "d‐Galactofuranose in the N‐linked sugar chain of a glycopeptide from Ascobolus furfuraceus" . European Journal of Biochemistry, vol. 165, no. 2, 1987, pp. 327-332.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb11445.x
---------- VANCOUVER ----------
GROISMAN, J.F., de LEDERKREMER, R.M. d‐Galactofuranose in the N‐linked sugar chain of a glycopeptide from Ascobolus furfuraceus. Eur. J. Biochem. 1987;165(2):327-332.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb11445.x