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Abstract:

Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system shows highly glucosylated, labeled bands, whose apparent molecular masses change with the acrylamide concentration in the gels. The long carbohydrate chains synthesized during the second step arise from the sequential addition of glucosyl moieties to the glucoprotein formed during the first step. A deglucosylation experiment confirms that the product of the reconstituted system originates from the 38‐kDa glucosylated component of the reaction 1 product by the addition of β‐amylase‐sensitive glucosyl moieties. Our data suggest that specific phosphorylases and starch synthetases are found in potato tuber, which are capable of utilizing reaction 1 product as primer for the synthesis of protein‐bound glucan. Copyright © 1987, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan
Autor:MORENO, S.; CARDINI, C.E.; TANDECARZ, J.S.
Filiación:Instituto de Investigaciones Bioquimicas ‘Fundacion Campomar’, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:1,4 glucan; 1,4-glucan; beta amylase; glucan; phosphorylase; starch synthase; vegetable protein; article; biosynthesis; catalysis; fluorography; metabolism; polyacrylamide gel electrophoresis; potato; protein binding; beta-Amylase; Catalysis; Electrophoresis, Polyacrylamide Gel; Glucans; Phosphorylases; Photofluorography; Plant Proteins; Potatoes; Protein Binding; Starch Synthase; Support, Non-U.S. Gov't
Año:1987
Volumen:162
Número:3
Página de inicio:609
Página de fin:614
DOI: http://dx.doi.org/10.1111/j.1432-1033.1987.tb10682.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:beta amylase, 9000-91-3; glucan, 9012-72-0, 9037-91-6; phosphorylase, 9035-74-9; starch synthase, 37338-93-5; 1,4-glucan, 9051-96-1; beta-Amylase, EC 3.2.1.2; Glucans; Phosphorylases, EC 2.4.1.-; Plant Proteins; Starch Synthase, EC 2.4.1.21
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v162_n3_p609_MORENO

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Citas:

---------- APA ----------
MORENO, S., CARDINI, C.E. & TANDECARZ, J.S. (1987) . α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan. European Journal of Biochemistry, 162(3), 609-614.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb10682.x
---------- CHICAGO ----------
MORENO, S., CARDINI, C.E., TANDECARZ, J.S. "α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan" . European Journal of Biochemistry 162, no. 3 (1987) : 609-614.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb10682.x
---------- MLA ----------
MORENO, S., CARDINI, C.E., TANDECARZ, J.S. "α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan" . European Journal of Biochemistry, vol. 162, no. 3, 1987, pp. 609-614.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb10682.x
---------- VANCOUVER ----------
MORENO, S., CARDINI, C.E., TANDECARZ, J.S. α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan. Eur. J. Biochem. 1987;162(3):609-614.
http://dx.doi.org/10.1111/j.1432-1033.1987.tb10682.x