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Abstract:

A partially purified glycogen synthase from rat cardiac muscle transferred glucosyl residues from UDP‐[14C]glucose to an endogenous protein acceptor in the absence of added primer. After native gel electrophoresis of the enzyme preparation, unprimed activity was detected. Primer‐dependent and independent activities were found in the same position. After denaturing gel electrophoresis of the reaction products, radioactivity comigrated with protein. Pulse‐chase experiments showed that the size of the reaction products increased as a function of time. These products were degraded by amyloglucosidase, thus suggesting that glycogen‐like molecules had grown on the protein acceptor. The activity of the enzyme was markedly reduced upon preincubation with α‐amylase. Therefore, preformed protein‐bound α‐1,4‐glucans were acting as primers. The glucoprotein acceptor may be a protein strongly associated with glycogen synthease, or alternatively, the enzyme itself. Copyright © 1986, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:The initiation of glycogen biosynthesis in rat heart α‐1,4 glucans tightly associated with glycogen synthase
Autor:BLUMENFELD, M.L.; KRISMAN, C.R.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Facultad de Ciencias Exactas y Naturales
CONICET, Buenos Aires, Argentina
Palabras clave:glycogen; glycogen synthase; alpha 1,4 glucan; animal cell; biosynthesis; heart; nonhuman; priority journal; rat; alpha-Amylase; Animal; Binding Sites; Electrophoresis, Polyacrylamide Gel; Glucans; Glucose; Glycogen; Glycogen Synthase; Myocardium; Protein Binding; Rats; Solubility; Support, Non-U.S. Gov't
Año:1986
Volumen:156
Número:1
Página de inicio:163
Página de fin:169
DOI: http://dx.doi.org/10.1111/j.1432-1033.1986.tb09562.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:glycogen synthase, 9033-05-0; glycogen, 9005-79-2; 1,4-glucan, 9051-96-1; alpha-Amylase, EC 3.2.1.1; Glucans; Glucose, 50-99-7; Glycogen Synthase, EC 2.4.1.11; Glycogen, 9005-79-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v156_n1_p163_BLUMENFELD

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Citas:

---------- APA ----------
BLUMENFELD, M.L. & KRISMAN, C.R. (1986) . The initiation of glycogen biosynthesis in rat heart α‐1,4 glucans tightly associated with glycogen synthase. European Journal of Biochemistry, 156(1), 163-169.
http://dx.doi.org/10.1111/j.1432-1033.1986.tb09562.x
---------- CHICAGO ----------
BLUMENFELD, M.L., KRISMAN, C.R. "The initiation of glycogen biosynthesis in rat heart α‐1,4 glucans tightly associated with glycogen synthase" . European Journal of Biochemistry 156, no. 1 (1986) : 163-169.
http://dx.doi.org/10.1111/j.1432-1033.1986.tb09562.x
---------- MLA ----------
BLUMENFELD, M.L., KRISMAN, C.R. "The initiation of glycogen biosynthesis in rat heart α‐1,4 glucans tightly associated with glycogen synthase" . European Journal of Biochemistry, vol. 156, no. 1, 1986, pp. 163-169.
http://dx.doi.org/10.1111/j.1432-1033.1986.tb09562.x
---------- VANCOUVER ----------
BLUMENFELD, M.L., KRISMAN, C.R. The initiation of glycogen biosynthesis in rat heart α‐1,4 glucans tightly associated with glycogen synthase. Eur. J. Biochem. 1986;156(1):163-169.
http://dx.doi.org/10.1111/j.1432-1033.1986.tb09562.x