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Abstract:

Dolichyl‐phosphate phosphatase and dolichyl‐diphosphate phosphatase activities of a liver‐cell microsomal preparation were solubilized by treatment with Triton X‐100. The I00000 × g supernatant was then passed through a column of Sepharose‐4B – concanavalin A. Both enzyme activities were found in the percolate. This treatment eliminated inhibition by ATP and glucose 6‐phosphate in both phosphatase activities. In each case the activities were inhibited by higher concentrations of enzyme preparation due to the presence of phospho‐ lipids. The inhibitory effects of either phosphatidylcholine or phosphatidylethanolamine were due to competition for detergent. On the other hand, the effect produced by phosphatidic acid appeared to be different, since it did not change the optimal concentration of Triton X‐100 for the two enzymes. Dolichyl‐phosphate phosphatase was strongly inhibited by both Pi and PPi, whereas dolichyl‐diphosphate phosphatase was only slightly inhibited by Pi and not at all by PPi. Dolichyl‐diphosphate phosphatase was more inhibited by divalent cations than dolichyl‐phosphate phos‐ phatase. The apparent Km of dolichyl‐phosphate phosphatase for dolichyl phosphate was 0.15 mM. Dolichol also inhibited dolichyl‐phosphate phosphatase, but it produced a stronger inhibition on dolichyl‐diphosphate phosphatase. The inhibitory effect of dolichol was not entirely due to detergent competition. Copyright © 1982, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Dolichyl‐Phosphate Phosphatase and Dolichyl‐Diphosphate Phosphatase in Rat‐Liver Microsomes
Autor:BELOCOPITOW, E.; BOSCOBOINIK, D.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundacion Campomar', Obligado, Buenos Aires, RA-1428, Argentina
Palabras clave:enzyme; animal experiment; dolichol diphosphatase; dolichyl phosphatase; liver; liver microsome; rat; Animal; Hydrogen-Ion Concentration; Kinetics; Microsomes, Liver; Phosphodiesterase Inhibitors; Phospholipids; Phosphoric Monoester Hydrolases; Pyrophosphatases; Rats; Substrate Specificity; Temperature
Año:1982
Volumen:125
Número:1
Página de inicio:167
Página de fin:173
DOI: http://dx.doi.org/10.1111/j.1432-1033.1982.tb06664.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:dolichyl-phosphatase, EC 3.1.3.51; dolichyldiphosphatase, EC 3.6.1.43; Phosphodiesterase Inhibitors; Phospholipids; Phosphoric Monoester Hydrolases, EC 3.1.3; Pyrophosphatases, EC 3.6.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v125_n1_p167_BELOCOPITOW

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Citas:

---------- APA ----------
BELOCOPITOW, E. & BOSCOBOINIK, D. (1982) . Dolichyl‐Phosphate Phosphatase and Dolichyl‐Diphosphate Phosphatase in Rat‐Liver Microsomes. European Journal of Biochemistry, 125(1), 167-173.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06664.x
---------- CHICAGO ----------
BELOCOPITOW, E., BOSCOBOINIK, D. "Dolichyl‐Phosphate Phosphatase and Dolichyl‐Diphosphate Phosphatase in Rat‐Liver Microsomes" . European Journal of Biochemistry 125, no. 1 (1982) : 167-173.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06664.x
---------- MLA ----------
BELOCOPITOW, E., BOSCOBOINIK, D. "Dolichyl‐Phosphate Phosphatase and Dolichyl‐Diphosphate Phosphatase in Rat‐Liver Microsomes" . European Journal of Biochemistry, vol. 125, no. 1, 1982, pp. 167-173.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06664.x
---------- VANCOUVER ----------
BELOCOPITOW, E., BOSCOBOINIK, D. Dolichyl‐Phosphate Phosphatase and Dolichyl‐Diphosphate Phosphatase in Rat‐Liver Microsomes. Eur. J. Biochem. 1982;125(1):167-173.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06664.x