Abstract:
The sequential synthesis in vitro of a heptasaccharide diphosphate prenol, containing glucose, mannose, glucuronic acid and rhamnose in the ratio 4:1:1:1 is described. The enzyme preparation consisted of EDTA‐treated Acetobacter xylinum cells and UDP‐glucose, GDO‐mannose, UDP‐glucuronic acid and TDP‐rhamnose were employed as sugar donors. The compounds soluble in chloroform/methanol/water (1:2:0.3) formed from incubations carried out under different conditions in the presence of a variety of combinations of the donors labeled with 14C, 3H or 32P were analysed by DEAE‐cellulose column chromatography, gel filtraton, partial acid hydrolysis, acetolysis, periodate oxidation, etc. The following structure is proposed for the most complex compound characterized: rhamnosyL(1 → 6)‐β‐glucosyl‐(1 → 6)‐α‐glucosyl‐(1 → 4)‐β‐glucuronyl‐(1 → 6)‐β‐mannosyl‐(1 → 3)‐β‐glucosyl‐(1 → 4)‐α‐glucosyl diphosphate prenol. The smaller oligosaccharide diphosphate prenols formed as intermediate steps are also characterized in this or in previous work [Garcia, R. C., Recondo, E. and Dankert, M. A. (1974) Eur. J. Biochem. 43, 93–105; Couso, R. O., Ielpi, L., and Damkert, M. A. (1980) Arch. Biochem. Biophys. 204, 434–443]. The role of these compounds in the biosynthesis of a complex exopolysaccharide that this microorganism forms in addition to cellulose is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved
Registro:
Documento: |
Artículo
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Título: | Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol |
Autor: | COUSO, R.O.; IELPI, L.; GARCIA, R.C.; DANKERT, M.A. |
Filiación: | Instituto de Investigaciones Bioquimicas 'Fundacion Campomar, Obligado 2490, Buenos Aires, RA-1428, Argentina
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Palabras clave: | polysaccharide; acetobacter xylinum; animal experiment; in vitro study; Acetobacter; Chemistry; Hydrolysis; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Polysaccharides, Bacterial; Rhamnose |
Año: | 1982
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Volumen: | 123
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Número: | 3
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Página de inicio: | 617
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Página de fin: | 627
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DOI: |
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06577.x |
Título revista: | European Journal of Biochemistry
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Título revista abreviado: | Eur. J. Biochem.
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ISSN: | 00142956
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CAS: | Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Polysaccharides, Bacterial; Rhamnose, 10485-94-6
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v123_n3_p617_COUSO |
Referencias:
- Couso, R.O., Ielpi, L., Dankert, M.A., (1980) Arch, Biochem. Biophys., 204, pp. 434-443
- Hestrin, S., Aschner, M., Mager, J., (1947) Nature (Lond.), 159, pp. 64-65
- Garcia, R.C., Recondo, E., Dnakert, M.A., (1974) Eur. J. Biochem., 43, pp. 93-105
- Okazaki, R., Okazaki, T., Strominert, J.L., Michelson, A.M., (1962) J. Biol. Chem., 237, pp. 3014-3026
- Bernstein, R.L., Robbins, P.W., (1965) J. Biol. Chem., 240, pp. 391-397
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
- de Iannino, N.I., Staneloni, R.J., Behrens, N.H., Dankert, M.A., (1979) FEBS Lett., 99, pp. 73-76
- Bray, G.A., (1960) Anal. Biochem., 1, pp. 279-285
- Davinson, E.A., (1966) Methods Enzymol., 8, pp. 52-60
- Locourek, J., Ballou, C.E., (1969) J. Bacteriol., 100, pp. 1175-1181
- Fales, F.W., (1959) Anal. Chem., 31, pp. 1898-1900
- Pont Lezica, R., Brett, C.T., Romero, P., Dankert, M.A., (1975) Biochem.Biophys. Res. Commun, 66, pp. 980-987
- Goldstein, I.J., Hay, G.W., Lewis, B.A., Smith, F., (1975) Methods Carbohydr. Chem., 5, pp. 361-370
- Ielpi, L., Couso, R.O., Dankert, M.A., (1981) Biochem. Biophys. Res. Commun., 102, pp. 1400-1408
- Taylor, L.R., Conrad, H.E., (1972) Biochemistry, 11, pp. 1383-1388
- Recondo, E., Goncalves, I.R.J., Dankert, M.A., (1964) J. Chromatogr., 16, pp. 415-416
- Foster, A.B., (1957) Adv. Carbohydr. Chem., 12, pp. 81-115
- Bourne, E.J., Hutson, D.H., Weigel, H., 7. Complexes between molybdate and acyclic polyhydroxy-compounds (1961) Journal of the Chemical Society (Resumed), 1, pp. 35-38
- Paladini, A.C., Leloir, L.F., (1952) Biochem. J., 51, pp. 426-430
- Jeanes, A., Wise, C.S., Dimler, R.J., (1951) Anal. Chem., 23, pp. 415-420
- Treveljan, W.E., Procter, D.P., Harrison, J.S., Detection of Sugars on Paper Chromatograms (1950) Nature, 166, pp. 444-445
- Dankert, M.A., Wright, A., Kelley, W.S., Robbins, P.W., (1966) Arch. Biochem. Biophys., 116, pp. 425-435
- Ielpi, L., Couso, R.O., Dankert, M.A., (1981) FEBS Lett., 130, pp. 253-256
- Smith, F., Montgomery, R., (1956) Methods of Biochemical Analysis, 3, pp. 154-157. , Glick, D., Interscince, New York, London
- Keglevic, D., (1979) Adv. Carbohydr. Chem. Biochem., 36, pp. 57-134
- Elderfield, R.C., (1945) Adv. Carbohydr. Chem., 1, pp. 147-150
- Romero, P., Garcia, R.C., Dankert, M.A., (1977) Mol. Cell. Biochem., 16, pp. 205-212
- Takayama, K., Schnoes, H.K., Semmler, E.J., (1973) Biochim. Biophys. Acta, 316, pp. 212-221
- Lennarz, W.J., Scher, M.G., (1972) Biochim. Biophys. Acta, 265, pp. 417-441
- Lahav, M., Chiu, T.M., Lennarz, W.J., (1979) J. Biol. Chem., 244, pp. 5890-5898
- Wright, A., (1971) J Bacteriol., 105, pp. 927-936
- Nikaido, H., Nikaido, K., Nakae, T., Maketa, P.H., (1971) J. Biol. Chem., 246, pp. 3902-3911
- Dekker, R.F.H., Rietschel, E.Th., Sandermann, H., Jr, (1977) Arch. Microbiol., 115, pp. 353-357
- Glaser, L., The synthesis of cellulose in cell-free extracts of Acetobacter xylinum. (1958) J Biol Chem, 232, pp. 627-636
- Sandermann, H., Jr, Dekker, R.F.H., (1979) FEBS Lett., 107, pp. 237-240
- Swissa, M., Aloni, Y., Weimhouse, H., Benziman, M., (1980) J. Bacteriol., 143, pp. 1142-1150
- Marx‐Fifini, M., Morris, E.R., (1977) Biochim. Biophys. Acta, 338, pp. 382-393
- Dea, I.C.M., Morris, E.R., Extracellular Microbiol Polyscaccharides (1977) American chemical Society Symposium Series, 45, pp. 174-182. , Stanfod, P. A., Lakin, A., eds, pp., American Chemical Society, Washington, DC
Citas:
---------- APA ----------
COUSO, R.O., IELPI, L., GARCIA, R.C. & DANKERT, M.A.
(1982)
. Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol. European Journal of Biochemistry, 123(3), 617-627.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06577.x---------- CHICAGO ----------
COUSO, R.O., IELPI, L., GARCIA, R.C., DANKERT, M.A.
"Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol"
. European Journal of Biochemistry 123, no. 3
(1982) : 617-627.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06577.x---------- MLA ----------
COUSO, R.O., IELPI, L., GARCIA, R.C., DANKERT, M.A.
"Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol"
. European Journal of Biochemistry, vol. 123, no. 3, 1982, pp. 617-627.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06577.x---------- VANCOUVER ----------
COUSO, R.O., IELPI, L., GARCIA, R.C., DANKERT, M.A. Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol. Eur. J. Biochem. 1982;123(3):617-627.
http://dx.doi.org/10.1111/j.1432-1033.1982.tb06577.x