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Abstract:

The effect of streptomycin on polypeptide synthesis in vivo and in vitro has been investigated using polyamine auxotrophic mutants of Escherichia coli grown in the presence or in the absence of putrescine. We found that streptomycin caused a marked inhibition of protein synthesis in polyamine‐supplemented cells whereas bacteria starved for polyamines were less sensitive to the action of the antibiotic. Neomycin, kanamycin and kasugamycin had a behaviour similar to streptomycin while spectinomycin, gentamicin and tetracycline, brought about a strong inhibition of protein synthesis both in polyamine‐starved and unstarved bacteria. The increase of misreading induced by the addition of streptomycin in vitro was higher in extracts derived from bacteria cultivated in the presence of polyamines. This effect was observed in cell‐free systems of streptomycin‐sensitive and resistant strains. In contrast, spermidine added in vitro caused an improvement in the accuracy of translation. Analysis of sodium dodecyl sulphate; polyacrylamide gel electrophoresis of the labelled polypeptides synthesized in vivo seems to indicate that the starvation for polyamine or the presence of streptomycin may lead to premature termination with the appearance of unfinished peptide chains. Copyright © 1981, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Poly amine Requirement for Streptomycin Action on Protein Synthesis in Bacteria
Autor:GOLDEMBERG, S.H.; ALGRANATI, I.D.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Compomar, Obligado 2490, Buenos Aires, RA-1428, Argentina
Palabras clave:gentamicin; kanamycin; kasugamycin; neomycin; polyamine; radioisotope; spectinomycin; streptomycin; tetracycline; animal experiment; drug comparison; drug sensitivity; escherichia coli; in vitro study; leucine c 14; phenylalanine c 14; protein synthesis; Aminoglycosides; Antibiotics; Bacterial Proteins; Drug Interactions; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Polyamines; Putrescine; Spermidine; Streptomycin; Support, Non-U.S. Gov't
Año:1981
Volumen:117
Número:2
Página de inicio:251
Página de fin:255
DOI: http://dx.doi.org/10.1111/j.1432-1033.1981.tb06330.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; kasugamycin, 6980-18-3; neomycin, 11004-65-2, 1404-04-2, 1405-10-3, 8026-22-0; spectinomycin, 1695-77-8, 21736-83-4, 23312-56-3; streptomycin, 57-92-1; tetracycline, 23843-90-5, 60-54-8, 64-75-5; Aminoglycosides; Antibiotics; Bacterial Proteins; Polyamines; Putrescine, 110-60-1; Spermidine, 124-20-9; Streptomycin, 57-92-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v117_n2_p251_GOLDEMBERG

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Citas:

---------- APA ----------
GOLDEMBERG, S.H. & ALGRANATI, I.D. (1981) . Poly amine Requirement for Streptomycin Action on Protein Synthesis in Bacteria. European Journal of Biochemistry, 117(2), 251-255.
http://dx.doi.org/10.1111/j.1432-1033.1981.tb06330.x
---------- CHICAGO ----------
GOLDEMBERG, S.H., ALGRANATI, I.D. "Poly amine Requirement for Streptomycin Action on Protein Synthesis in Bacteria" . European Journal of Biochemistry 117, no. 2 (1981) : 251-255.
http://dx.doi.org/10.1111/j.1432-1033.1981.tb06330.x
---------- MLA ----------
GOLDEMBERG, S.H., ALGRANATI, I.D. "Poly amine Requirement for Streptomycin Action on Protein Synthesis in Bacteria" . European Journal of Biochemistry, vol. 117, no. 2, 1981, pp. 251-255.
http://dx.doi.org/10.1111/j.1432-1033.1981.tb06330.x
---------- VANCOUVER ----------
GOLDEMBERG, S.H., ALGRANATI, I.D. Poly amine Requirement for Streptomycin Action on Protein Synthesis in Bacteria. Eur. J. Biochem. 1981;117(2):251-255.
http://dx.doi.org/10.1111/j.1432-1033.1981.tb06330.x