Artículo
MEDRANO, E.; IUJVIDIN, S.; MORDOH, J. "Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine" (1981) European Journal of Biochemistry. 116(2):249-253
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Abstract:
When phytohemagglutinin‐stimulated lymphocytes are labelled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as thin‐layer chromatography and alkaline hydrolysis of the product obtained after labelling of stimulated lymphocytes with [14C]glycerol and [3H]dCyd, suggest that the product synthesized is phosphatidyl‐dCMP. This compound is synthesized in much smaller amounts than when permeabilized lymphocytes and [3H]dCTP as a precursor are used. The synthesis of phosphatidyl‐dCMP is highly enhanced by chlorpromazine, phosphatidic acid and arachidonic acid, whereas addition of inositol diminishes the amount of detected phosphatidyl‐dCMP. It is suggested that this compound would be a precursor of phosphatidylinositol. Copyright © 1981, Wiley Blackwell. All rights reserved
Registro:
| Documento: | Artículo |
| Título: | Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| Autor: | MEDRANO, E.; IUJVIDIN, S.; MORDOH, J. |
| Filiación: | Instituto de Investigaciones Bioquímicas 'Fundacion Campomar', Obligado, Buenos Aires, RA-1428, Argentina Fundación Centro de Investigaciones Medicas Albert Einstein, Luis Viale 2831, Buenos Aires, RA-1416, Argentina |
| Palabras clave: | chlorpromazine; phosphatidylinositol; radioisotope; biosynthesis; blood and hemopoietic system; deoxycytidine h 3; human cell; in vitro study; lymphatic system; lymphocyte; normal human; phosphatidyldeoxycytidine phosphate; Chlorpromazine; Deoxycytidine; Deoxycytidine Monophosphate; Deoxycytosine Nucleotides; Dose-Response Relationship, Drug; Human; In Vitro; Kinetics; Lymphocyte Activation; Lymphocytes; Phosphatidic Acids; Phytohemagglutinins; Support, Non-U.S. Gov't |
| Año: | 1981 |
| Volumen: | 116 |
| Número: | 2 |
| Página de inicio: | 249 |
| Página de fin: | 253 |
| DOI: | http://dx.doi.org/10.1111/j.1432-1033.1981.tb05326.x |
| Título revista: | European Journal of Biochemistry |
| Título revista abreviado: | Eur. J. Biochem. |
| ISSN: | 00142956 |
| CAS: | chlorpromazine, 50-53-3, 69-09-0; Chlorpromazine, 50-53-3; Deoxycytidine Monophosphate, 1032-65-1; Deoxycytidine, 951-77-9; Deoxycytosine Nucleotides; Phosphatidic Acids; phosphatidyl-dCMP; Phytohemagglutinins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v116_n2_p249_MEDRANO |
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Citas:
---------- APA ----------
MEDRANO, E., IUJVIDIN, S. & MORDOH, J. (1981) . Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine. European Journal of Biochemistry, 116(2), 249-253.http://dx.doi.org/10.1111/j.1432-1033.1981.tb05326.x
---------- CHICAGO ----------
MEDRANO, E., IUJVIDIN, S., MORDOH, J. "Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine" . European Journal of Biochemistry 116, no. 2 (1981) : 249-253.http://dx.doi.org/10.1111/j.1432-1033.1981.tb05326.x
---------- MLA ----------
MEDRANO, E., IUJVIDIN, S., MORDOH, J. "Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine" . European Journal of Biochemistry, vol. 116, no. 2, 1981, pp. 249-253.http://dx.doi.org/10.1111/j.1432-1033.1981.tb05326.x
---------- VANCOUVER ----------
MEDRANO, E., IUJVIDIN, S., MORDOH, J. Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine. Eur. J. Biochem. 1981;116(2):249-253.http://dx.doi.org/10.1111/j.1432-1033.1981.tb05326.x
