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Abstract:

Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal of glucose from the glucose‐containing oligosaccharide. One acts on the oligosaccharide containing three glucose residues and another on the oligosaccharide which has one or two glucoses. The glucosidase which acts on (Glc)2(Man)9(GlcNAc)2 could be purified with a ConcanavalinA—Sepharose column followed by electrofocusing. This purified preparation was active on the oligosaccharide containing one or two glucoses. Heat inactivation and inhibition by disaccharides was parallel for both activities. Inhibition of the glucosidase active on (Glc)3(Man)9(GlcNAc)2 was obtained with kojibiose which has an α 1–2 linkage, while the glucosidase acting on (Glc)1–2(Man)9‐(GlcNAc)2 was inhibited by nigerose (α 1–3 linkage), maltose (α 1–4 linkage) and glucose at a higher concentration. None of the β anomers inhibited. These results are consistent with an α configuration of the three glucoses of the dolichyl‐dinhosphate‐linked oligosaccharide. Kojibiose was found to inhibit glucosidase action not only on the free oligosaccharide but also on protein‐bound one. Copyright © 1980, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides
Autor:UGALDE, R.A.; STANELONI, R.J.; LELOIR, L.F.
Filiación:Instituto de Investigaciones Bioquímicas "Fundación Campomar, Obligado 2490, Buenos Aires, RA-1428, Argentina
Palabras clave:disaccharide; glucosidase; glycoprotein; isoprenoid phosphate sugar; kojibiose; animal; article; enzymology; isolation and purification; liver microsome; metabolism; rat; Animal; Disaccharides; Glucosidases; Glycoproteins; Microsomes, Liver; Polyisoprenyl Phosphate Oligosaccharides; Rats
Año:1980
Volumen:113
Número:1
Página de inicio:97
Página de fin:103
DOI: http://dx.doi.org/10.1111/j.1432-1033.1980.tb06144.x
Título revista:European Journal of Biochemistry
Título revista abreviado:Eur. J. Biochem.
ISSN:00142956
CAS:glucosidase, 9033-06-1; Disaccharides; Glucosidases, EC 3.2.1.-; Glycoproteins; kojibiose, 2140-29-6; Polyisoprenyl Phosphate Oligosaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v113_n1_p97_UGALDE

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Citas:

---------- APA ----------
UGALDE, R.A., STANELONI, R.J. & LELOIR, L.F. (1980) . Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides. European Journal of Biochemistry, 113(1), 97-103.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06144.x
---------- CHICAGO ----------
UGALDE, R.A., STANELONI, R.J., LELOIR, L.F. "Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides" . European Journal of Biochemistry 113, no. 1 (1980) : 97-103.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06144.x
---------- MLA ----------
UGALDE, R.A., STANELONI, R.J., LELOIR, L.F. "Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides" . European Journal of Biochemistry, vol. 113, no. 1, 1980, pp. 97-103.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06144.x
---------- VANCOUVER ----------
UGALDE, R.A., STANELONI, R.J., LELOIR, L.F. Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides. Eur. J. Biochem. 1980;113(1):97-103.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06144.x