Artículo
STANELONI, R.J.; UGALDE, R.A.; LELOIR, L.F. "Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein" (1980) European Journal of Biochemistry. 105(2):275-278
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Abstract:
The glycosylation of asparagine residues in proteins is known to occur by transfer from a dolichyl diphosphate oligosaccharide containing glucose. Paper chromatography allowed the separation of oligosaccharides (obtained by acid hydrolysis of the dolichyl diphosphate derivative) containing 1, 2 and 3 glucose residues. Using this procedure it was found that the addition of all three glucoses to the dolichyl diphosphate oligosaccharide occur with dolichyl phosphate glucose as donor. Furthermore only the compound with three glucoses was used as donor in the transfer to protein. The addition of glucose to exogenous dolichyldiphosphate oligosaccharide labelled by transfer from radioactive guanosine diphosphate mannose was detected. Copyright © 1980, Wiley Blackwell. All rights reserved
Registro:
| Documento: | Artículo |
| Título: | Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
| Autor: | STANELONI, R.J.; UGALDE, R.A.; LELOIR, L.F. |
| Filiación: | Instituto de Investigaciones Bioquímicas "Fundacion Campomar", Obligado 2490, Buenos Aires, RA-1428, Argentina |
| Palabras clave: | dolichol D glucosylmonophosphate; dolichol-D-glucosylmonophosphate; dolichyl diphosphate oligosaccharides; glucose; isoprenoid phosphate sugar; protein; animal; article; liver microsome; metabolism; paper chromatography; rat; Animal; Chromatography, Paper; Glucose; Microsomes, Liver; Polyisoprenyl Phosphate Monosaccharides; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Proteins; Rats |
| Año: | 1980 |
| Volumen: | 105 |
| Número: | 2 |
| Página de inicio: | 275 |
| Página de fin: | 278 |
| DOI: | http://dx.doi.org/10.1111/j.1432-1033.1980.tb04498.x |
| Título revista: | European Journal of Biochemistry |
| Título revista abreviado: | Eur. J. Biochem. |
| ISSN: | 00142956 |
| CAS: | glucose, 50-99-7, 84778-64-3; protein, 67254-75-5; dolichol-D-glucosylmonophosphate, 55607-88-0; dolichyl diphosphate oligosaccharides; Glucose, 50-99-7; Polyisoprenyl Phosphate Monosaccharides; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v105_n2_p275_STANELONI |
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Citas:
---------- APA ----------
STANELONI, R.J., UGALDE, R.A. & LELOIR, L.F. (1980) . Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein. European Journal of Biochemistry, 105(2), 275-278.http://dx.doi.org/10.1111/j.1432-1033.1980.tb04498.x
---------- CHICAGO ----------
STANELONI, R.J., UGALDE, R.A., LELOIR, L.F. "Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein" . European Journal of Biochemistry 105, no. 2 (1980) : 275-278.http://dx.doi.org/10.1111/j.1432-1033.1980.tb04498.x
---------- MLA ----------
STANELONI, R.J., UGALDE, R.A., LELOIR, L.F. "Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein" . European Journal of Biochemistry, vol. 105, no. 2, 1980, pp. 275-278.http://dx.doi.org/10.1111/j.1432-1033.1980.tb04498.x
---------- VANCOUVER ----------
STANELONI, R.J., UGALDE, R.A., LELOIR, L.F. Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein. Eur. J. Biochem. 1980;105(2):275-278.http://dx.doi.org/10.1111/j.1432-1033.1980.tb04498.x
