Abstract:
Biosynthesis and regulation of expression of transplantation antigens as detected by a monoclonal antibody to HLA‐A,B,C antigens (human leucocytic antigen) and a polyclonal antiserum to β2‐micro‐globulin have been investigated using radioactive amino acids and sugars to label human lymphoid cells. We found unbalanced synthesis of HLA heavy chains and β2‐microglobulin, the latter being in excess and secreted to the extracellular medium. In DAUDI cells, which are defective in β2‐microglobulin, no HLA‐A,B,C could be detected intracellularly even in the presence of added β2‐microglobulin. Treatment of BRI‐8 cells with tunicamycin, an antibiotic which inhibits glycosylation of polypeptides, almost had no effect on the levels of β2‐microglobulin, while it markedly decreased that of HLA heavy chains, both on the cell surface and intracellularly. Glycosylation of the HLA heavy chains appeared to be an essential requirement for the normal expression of HLA‐A,B,C antigens. The translation in vitro in a messenger‐dependent reticulocyte system with total polysomes obtained from BRI‐8 cells showed that β2‐microglobulin was synthesized as a precursor. This larger polypeptide was converted into mature β2‐microglobulin when protein synthesis was performed with microsomes instead of polysomes. Copyright © 1980, Wiley Blackwell. All rights reserved
Registro:
Documento: |
Artículo
|
Título: | Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens |
Autor: | ALGRANATI, I.D.; MILSTEIN, C.; ZIEGLER, A. |
Filiación: | Instituto de Investigaciones Bioquímicas 'Fundacion Campomar', Obligado, Buenos Aires, RA-1428, Argentina Medical Research Council Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge, CB2 2QH, United Kingdom Abteilung Innere Medizin II, Medizinische Klinik, Eberhard-Karls-Universität Tübingen, Olfried-Müller-Straße 10, Tübingen, D-7400, Germany
|
Palabras clave: | histocompatibility antigen; HLA antigen; monoclonal antibody; heredity; human cell; lymphatic system; normal human; beta 2-Microglobulin; Beta-Globulins; Cell Line; Electrophoresis, Polyacrylamide Gel; Epitopes; Histocompatibility Antigens; Human; Lymphoid Tissue; Macromolecular Systems; Translation, Genetic; Tunicamycin |
Año: | 1980
|
Volumen: | 103
|
Número: | 1
|
Página de inicio: | 197
|
Página de fin: | 207
|
DOI: |
http://dx.doi.org/10.1111/j.1432-1033.1980.tb04304.x |
Título revista: | European Journal of Biochemistry
|
Título revista abreviado: | Eur. J. Biochem.
|
ISSN: | 00142956
|
CAS: | beta 2-Microglobulin; Beta-Globulins; Epitopes; Histocompatibility Antigens; Macromolecular Systems; Tunicamycin, 11089-65-9
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v103_n1_p197_ALGRANATI |
Referencias:
- Nakamuro, K., Tanigaki, N., Pressman, D., (1973) Proc. Natl Acad. Sci. U.S.A., 70, pp. 2863-2865
- Grey, H.M., Kubo, R.T., Colon, S.M., Poulik, M.D., Cress‐well, P., Springer, T., Turner, M., Strominger, J.L., (1973) J. Exp. Med., 138, pp. 1608-1612
- Silver, J., Hood, L., (1974) Nature (Lond.), 249, pp. 764-765
- Rask, L., Lindblom, J.B., Peterson, P.A., (1974) Nature (Lond.), 249, pp. 833-834
- Ziegler, A., Pink, J.R.L., (1975) Transplantation (Baltimore), 20, pp. 523-527
- Ziegler, A., Pink, R., (1976) J. Biol. Chem., 251, pp. 5391-5396
- Poulik, M.D., Bernoco, M., Bernoco, D., Ceppellini, R., (1973) Science (Wash. D.C.), 182, pp. 1352-1355
- Smithies, O., Poulik, M.D., (1972) Science (Wash. D.C.), 175, pp. 187-189
- Peterson, P.A., Cunningham, B.A., Berggard, I., Edelman, G.M., (1972) Proc. Natl Acad. Sci. U.S.A., 69, pp. 1697-1701
- Bodmer, W.F., (1977) The Harvey Lectures, , Academic Press, New York, in the press
- Goodfellow, P.N., Jones, E.A., van Heyningen, V., Solomon, E., Bobrow, M., Miggiano, V., Bodmer, W.F., (1975) Nature (Lond.), 254, pp. 267-269
- Cresswell, P., Turner, M.J., Strominger, J.L., (1973) Proc. Natl Acad. Sci. U.S.A., 70, pp. 1603-1607
- Parham, P., Alpert, B.N., Orr, H.T., Strominger, J.L., (1977) J. Biol. Chem., 252, pp. 7555-7567
- Götze, D., (1977) The Major Histocompatibility System in Man and Animals, , Springer Verlag, Berlin
- Vitetta, E.S., Capra, J.D., (1978) Adv. Immunol., 26, pp. 147-193
- Tada, N., Tanigaki, N., Pressman, D., (1978) J. Immunol., 120, pp. 513-519
- Fellous, M., Günther, E., Kemler, R., Wiels, J., Berger, R., Guenet, J.L., Jacob, H., Jacob, F., (1978) J. Exp. Med., 148, pp. 58-70
- Nakamuro, K., Tanigaki, N., Pressman, D., (1977) Immunology, 32, pp. 139-147
- Barnstable, C.J., Bodmer, W.F., Brown, G., Galfre, G., Milstein, C., Williams, A.F., Ziegler, A., (1978) Cell, 14, pp. 9-20
- Snary, D., Goodfellow, P., Hayman, M.J., Bodmer, W.F., Crumpton, M.J., (1974) Nature (Lond.), 247, pp. 457-461
- Klein, E., Klein, G., Nadkarni, J.S., Nadkarni, J.J., Wigzell, H., Clifford, P., (1968) Cancer Res., 28, pp. 1300-1310
- Ziegler, A., Milstein, C., (1979) Nature (Lond.), 279, pp. 243-244
- Ziegler, A., Harrison, S.C., Leberman, R., (1974) Virology, 59, pp. 509-515
- Bonner, W.M., Laskey, R.A., (1974) Eur. J. Biochem., 46, pp. 83-88
- Pelham, H.R.B., Jackson, R.J., (1976) Eur. J. Biochem., 67, pp. 247-256
- Hickman, S., Kuczycki, A., Jr, Lynch, R.G., Kornfeld, S., (1977) J. Biol. Chem., 252, pp. 4402-4408
- Hickman, S., Kornfeld, S., (1978) J. Immunol., 121, pp. 990-996
- McMichael, A.J., Pilch, J.R., Galfre, G., Mason, D.Y., Fabre, J.W., Milstein, C., (1979) Eur. J. Immunol., , in the press
- Gally, J.A., Edelman, G.M., (1972) Annu. Rev. Genet., 6, pp. 1-46
- Bodmer, W.F., (1972) Nature (Lond.), 237, pp. 139-145
- Bernier, G.M., Fanger, M.W., (1972) J. Immunol., 109, pp. 407-409
- Hütteroth, T.H., Cleve, H., Litwin, S.D., Poulik, M.D., (1973) J. Exp. Med., 137, pp. 838-843
- Jones, E.A., Goodfellow, P.N., Bodmer, J.G., Bodmer, W.F., (1975) Nature (Lond.), 256, pp. 650-652
- Zeuthen, J., Friedrich, U., Rosén, A., Klein, E., (1977) Immunogenetics, 4, pp. 567-579
- Klein, G., Terasaki, P., Billing, R., Honig, R., Jondal, M., Rosén, A., Zeuthen, J., Clements, G., (1977) Int. J. Cancer, 19, pp. 66-76
- Reisfeld, R.A., Servier, E.D., Pellegrino, M.A., Ferrone, S., Poulik, M.D., (1975) Immunogenetics, 2, pp. 183-197
- Arce‐Gómez, B., Jones, E.A., Barnstable, C.D., Solomon, E., Bodmer, W.F., (1978) Tissue Antigens, 11, pp. 96-112
- Marchalonis, J.J., Cone, R.E., Santer, V., (1971) Biochem. J., 124, pp. 921-927
- Takatsuki, A., Arima, K., Tamura, G., (1971) J. Antibiot. (Tokyo) Ser. A, 24, pp. 215-223
- Tkacz, J.S., Lampen, J.O., (1975) Biochem. Biophys. Res. Commun., 65, pp. 248-257
- Waechter, C.J., Lennarz, W.J., (1976) Annu. Rev. Biochem., 45, pp. 95-112
- Snary, D., Barnstable, C.J., Bodmer, W.F., Crumpton, M.J., (1977) Eur. J. Immunol., 8, pp. 580-585
- Garoff, H., Schwarz, R.T., (1978) Nature (Lond.), 274, pp. 487-489
- Hyman, R., Trowbridge, I., (1978) Conf. Cell Proliferation, 5. , in the press
- Williamson, A.R., Defence and Recognition (1973) Biochemistry Series I, 10, pp. 229-255. , Porter, R. R., Butterworths, London
- Springer, T.A., Strominger, J.L., (1976) Proc. Natl Acad. Sci. U.S.A., 73, pp. 2481-2485
- Ramasamy, R., Munro, A., Milstein, C., (1974) Nature (Lond.), 249, pp. 573-574
- Ploegh, H.L., Cannon, L.E., Strominger, J.L., (1979) Proc. Natl Acad. Sci. U.S.A., 76, pp. 2273-2277
Citas:
---------- APA ----------
ALGRANATI, I.D., MILSTEIN, C. & ZIEGLER, A.
(1980)
. Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens. European Journal of Biochemistry, 103(1), 197-207.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb04304.x---------- CHICAGO ----------
ALGRANATI, I.D., MILSTEIN, C., ZIEGLER, A.
"Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens"
. European Journal of Biochemistry 103, no. 1
(1980) : 197-207.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb04304.x---------- MLA ----------
ALGRANATI, I.D., MILSTEIN, C., ZIEGLER, A.
"Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens"
. European Journal of Biochemistry, vol. 103, no. 1, 1980, pp. 197-207.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb04304.x---------- VANCOUVER ----------
ALGRANATI, I.D., MILSTEIN, C., ZIEGLER, A. Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens. Eur. J. Biochem. 1980;103(1):197-207.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb04304.x