Artículo

Buggiano, V.; Petrillo, E.; Alló, M.; Lafaille, C.; Redal, M.A.; Alghamdi, M.A.; Khoder, M.I.; Shamy, M.; Muñoz, M.J.; Kornblihtt, A.R. "Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells" (2015) Environmental Research. 140:185-190
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Abstract:

Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, resulting from industrial pollution, inhibits expression and specifically affects alternative splicing at the 5' untranslated region of the mRNA encoding the bone morphogenetic protein BMP4 in human colon cells in culture. These effects are consistent with a previously reported role for BMP4 in preventing colon cancer development, suggesting that ingestion of particulate matter could contribute to the onset of colon cell proliferation. We also show that the underlying mechanism might involve changes in transcriptional elongation. This is the first study to demonstrate that particulate matter causes non-pleiotropic changes in alternative splicing. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
Autor:Buggiano, V.; Petrillo, E.; Alló, M.; Lafaille, C.; Redal, M.A.; Alghamdi, M.A.; Khoder, M.I.; Shamy, M.; Muñoz, M.J.; Kornblihtt, A.R.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología, Biología Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EHA, Argentina
Instituto de Ciencias Básicas y Medicina Experimental, Hospital Italiano de Buenos Aires, Argentina
Department of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia
Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah, Saudi Arabia
Max F. Perutz Laboratories, Medical University of Vienna, Vienna, A-1030, Austria
European Molecular Biology Laboratory, Meyerhofstrasse1, Heidelberg, 69117, Germany
Palabras clave:Air pollution; Alternative splicing; Bone morphogenetic protein; Colon cancer cells; Particulate matter; bone morphogenetic protein; messenger RNA; bone morphogenetic protein 4; messenger RNA; particulate matter; primer DNA; RNA precursor; atmospheric pollution; cancer; cytology; industrial waste; particulate matter; protein; RNA; 5' untranslated region; air pollution; alternative RNA splicing; Article; cancer cell culture; cancer cell line; cell proliferation; colon cancer; colon carcinogenesis; controlled study; human; human cell; particulate matter; priority journal; transcription elongation; alternative RNA splicing; colon tumor; drug effects; genetics; HEK293 cell line; nucleotide sequence; particulate matter; pathology; pharmacology; reverse transcription polymerase chain reaction; tumor cell line; Alternative Splicing; Base Sequence; Bone Morphogenetic Protein 4; Cell Line, Tumor; Colonic Neoplasms; DNA Primers; HEK293 Cells; Humans; Particulate Matter; Reverse Transcriptase Polymerase Chain Reaction; RNA Precursors; RNA, Messenger
Año:2015
Volumen:140
Página de inicio:185
Página de fin:190
DOI: http://dx.doi.org/10.1016/j.envres.2015.04.001
Título revista:Environmental Research
Título revista abreviado:Environ. Res.
ISSN:00139351
CODEN:ENVRA
CAS:Bone Morphogenetic Protein 4; DNA Primers; Particulate Matter; RNA Precursors; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00139351_v140_n_p185_Buggiano

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Citas:

---------- APA ----------
Buggiano, V., Petrillo, E., Alló, M., Lafaille, C., Redal, M.A., Alghamdi, M.A., Khoder, M.I.,..., Kornblihtt, A.R. (2015) . Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells. Environmental Research, 140, 185-190.
http://dx.doi.org/10.1016/j.envres.2015.04.001
---------- CHICAGO ----------
Buggiano, V., Petrillo, E., Alló, M., Lafaille, C., Redal, M.A., Alghamdi, M.A., et al. "Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells" . Environmental Research 140 (2015) : 185-190.
http://dx.doi.org/10.1016/j.envres.2015.04.001
---------- MLA ----------
Buggiano, V., Petrillo, E., Alló, M., Lafaille, C., Redal, M.A., Alghamdi, M.A., et al. "Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells" . Environmental Research, vol. 140, 2015, pp. 185-190.
http://dx.doi.org/10.1016/j.envres.2015.04.001
---------- VANCOUVER ----------
Buggiano, V., Petrillo, E., Alló, M., Lafaille, C., Redal, M.A., Alghamdi, M.A., et al. Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells. Environ. Res. 2015;140:185-190.
http://dx.doi.org/10.1016/j.envres.2015.04.001