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Abstract:

Quantitative trait loci (QTL) for thermotolerance were previously identified for adult flies in several mapping populations of Drosophila melanogaster Meigen (Diptera: Drosophilidae) in the laboratory. However, laboratory assays may not necessarily reflect the performance under heat stress in the field. For instance, do the heat-resistance QTL regions in the field match the QTL for thermotolerance in laboratory studies? To address this and related questions we used a set of recombinant inbred lines (RIL), which were originally used to identify QTL in the laboratory.We tested egg-to-adult survival (EAS) QTL in a field experiment under naturally varying heat-stress temperatures in fly cultures reared on a rotting fruit (banana) in summer. EAS under heat stress was found to be 3–69lower (depending on RIL) in the field than in the corresponding control at benign temperature (25 °C). Five QTL for EAS were significant in the field experiment under heat stress, four of them co-located with plasticity QTL, and none of theQTL was significant at control temperature. All significant QTL overlapped (co-localized) with thermotolerance QTL previously identified in the laboratory. A previously found QTL in the middle of chromosome 2 explained near 30% of the phenotypic variance in EAS under heat stress in previous studies in the laboratory, but this QTL explained only 8% of the EAS variation in our field assay. The largest effect on EAS was found for an X-linked QTL (cytological range 7B3-10C3) in the heat-stress field experiment, explaining a high percentage (14–45%) of the phenotypic variation in EAS. The ecological relevance ofQTL implicated in this study is discussed. © 2018 The Netherlands Entomological Society.

Registro:

Documento: Artículo
Título:Genetic variation for egg-to-adult survival in drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment
Autor:Borda, M.A.; Sambucetti, P.D.; Gomez, F.H.; Norry, F.M.
Filiación:Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, C-1428-EHA, Argentina
Instituto de Ecologıa, Genética y Evolución de Buenos Aires – CONICET (IEGEBA), Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Developmental survival; Diptera; Drosophilidae; Environmental stress; Field assay; Genotype-by-environment interaction; QTL; Quantitative trait loci; Thermal stress; Thermotolerance; Diptera; Drosophila melanogaster; Drosophilidae
Año:2018
Volumen:166
Número:10
Página de inicio:863
Página de fin:872
DOI: http://dx.doi.org/10.1111/eea.12728
Título revista:Entomologia Experimentalis et Applicata
Título revista abreviado:Entomol. Exp. Appl.
ISSN:00138703
CODEN:ETEAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00138703_v166_n10_p863_Borda

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Citas:

---------- APA ----------
Borda, M.A., Sambucetti, P.D., Gomez, F.H. & Norry, F.M. (2018) . Genetic variation for egg-to-adult survival in drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment. Entomologia Experimentalis et Applicata, 166(10), 863-872.
http://dx.doi.org/10.1111/eea.12728
---------- CHICAGO ----------
Borda, M.A., Sambucetti, P.D., Gomez, F.H., Norry, F.M. "Genetic variation for egg-to-adult survival in drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment" . Entomologia Experimentalis et Applicata 166, no. 10 (2018) : 863-872.
http://dx.doi.org/10.1111/eea.12728
---------- MLA ----------
Borda, M.A., Sambucetti, P.D., Gomez, F.H., Norry, F.M. "Genetic variation for egg-to-adult survival in drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment" . Entomologia Experimentalis et Applicata, vol. 166, no. 10, 2018, pp. 863-872.
http://dx.doi.org/10.1111/eea.12728
---------- VANCOUVER ----------
Borda, M.A., Sambucetti, P.D., Gomez, F.H., Norry, F.M. Genetic variation for egg-to-adult survival in drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment. Entomol. Exp. Appl. 2018;166(10):863-872.
http://dx.doi.org/10.1111/eea.12728