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Abstract:

Under illumination conditions, porphyrins generate cytotoxic radicals in cells. Our study evaluated the effects of haematoporphyrin IX (HP IX) in a laboratory population of male Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) during exposure to a low fluence rate (39 μE m−2 s−1) of light. We found that exposing flies to HP IX for at least 5 days was sufficient to cause irreversible damage that led to anticipated death, as also provoked by chronic exposure to the same concentration. To identify early indicators of the accelerated senescence, we analysed both in vitro and in vivo parameters. The thiobarbituric acid reactive substances content in the heads of treated flies revealed a significant increase in lipid hydroperoxides at day 10, whereas this occurred several days later in controls. In addition, a significant decrease in glycogen content was observed at 15 days of age, 5 days before the reduction observed in the control group. This decrease has been associated with a decline in locomotor activity. Differences in the distribution of flies in the rearing flasks were observed, reflecting an impairment of the motility and climbing capacity of HP IX-treated flies. This finding was also corroborated by a geotactic response assay (a rapid iterative negative geotaxis or RING assay). The results presented here demonstrate that low-lethal oxidative stress can anticipate the senescence of flies, which can be predicted using a simple and fast behavioural test, such as the RING assay. © 2017 The Netherlands Entomological Society

Registro:

Documento: Artículo
Título:Poor geotaxis correlated with haematoporphyrin-induced peroxidation of brain lipids as a predictor of medfly longevity reduction
Autor:Bochicchio, P.A.; Pérez, M.M.; Quesada-Allué, L.A.; Rabossi, A.
Filiación:IIBBA-CONICET, Av. Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Fundación Instituto Leloir, Av. Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Av. Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Palabras clave:anticipated death; Ceratitis capitata; Diptera; functional senescence; glycogen content; lipid peroxidation; negative geotaxis; photosensitizer; Tephritidae; bioassay; brain; correlation; damage; fly; induced response; light effect; lipid; longevity; mortality; motility; oxidation; oxidative stress; porphyrin; senescence; Ceratitis capitata; Diptera; Tephritidae
Año:2017
Volumen:164
Número:2
Página de inicio:131
Página de fin:140
DOI: http://dx.doi.org/10.1111/eea.12597
Título revista:Entomologia Experimentalis et Applicata
Título revista abreviado:Entomol. Exp. Appl.
ISSN:00138703
CODEN:ETEAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00138703_v164_n2_p131_Bochicchio

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Citas:

---------- APA ----------
Bochicchio, P.A., Pérez, M.M., Quesada-Allué, L.A. & Rabossi, A. (2017) . Poor geotaxis correlated with haematoporphyrin-induced peroxidation of brain lipids as a predictor of medfly longevity reduction. Entomologia Experimentalis et Applicata, 164(2), 131-140.
http://dx.doi.org/10.1111/eea.12597
---------- CHICAGO ----------
Bochicchio, P.A., Pérez, M.M., Quesada-Allué, L.A., Rabossi, A. "Poor geotaxis correlated with haematoporphyrin-induced peroxidation of brain lipids as a predictor of medfly longevity reduction" . Entomologia Experimentalis et Applicata 164, no. 2 (2017) : 131-140.
http://dx.doi.org/10.1111/eea.12597
---------- MLA ----------
Bochicchio, P.A., Pérez, M.M., Quesada-Allué, L.A., Rabossi, A. "Poor geotaxis correlated with haematoporphyrin-induced peroxidation of brain lipids as a predictor of medfly longevity reduction" . Entomologia Experimentalis et Applicata, vol. 164, no. 2, 2017, pp. 131-140.
http://dx.doi.org/10.1111/eea.12597
---------- VANCOUVER ----------
Bochicchio, P.A., Pérez, M.M., Quesada-Allué, L.A., Rabossi, A. Poor geotaxis correlated with haematoporphyrin-induced peroxidation of brain lipids as a predictor of medfly longevity reduction. Entomol. Exp. Appl. 2017;164(2):131-140.
http://dx.doi.org/10.1111/eea.12597