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Abstract:

DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society.

Registro:

Documento: Artículo
Título:Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
Autor:Lanzavecchia, S.; Remis, M.; Cladera, J.; Zandomeni, R.
Filiación:Laboratorio de Genética de Insectos de Importancia Económica, Instituto de Genetica 'Ewald A. Favret', Instituto Nacional de Tecnologia Agropecuaria (INTA), Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Castelar, Buenos Aires, Argentina
Laboratorio de Genética de Poblaciones Aplicada, Departamento de Ecología, Universidad de Buenos Aires, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Laboratorio de Alta Complejidad, Instituto de Microbiología y Zoología Agrícola, CICVyA, INTA, Castelar, Buenos Aires, Argentina
Palabras clave:Diptera; EPIC-PCR; Exon priming intron crossing-polymerase chain reaction; Gene flow; Medfly; Migration patterns; Molecular markers; Population genetics; Tephritidae; allele; biomarker; fly; gene flow; genetic analysis; heterozygosity; migration; polymerase chain reaction; polymorphism; Ceratitis capitata; Diptera; Hexapoda; Tephritidae
Año:2010
Volumen:136
Número:1
Página de inicio:53
Página de fin:65
DOI: http://dx.doi.org/10.1111/j.1570-7458.2010.01002.x
Título revista:Entomologia Experimentalis et Applicata
Título revista abreviado:Entomol. Exp. Appl.
ISSN:00138703
CODEN:ETEAA
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00138703_v136_n1_p53_Lanzavecchia.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00138703_v136_n1_p53_Lanzavecchia

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Citas:

---------- APA ----------
Lanzavecchia, S., Remis, M., Cladera, J. & Zandomeni, R. (2010) . Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata. Entomologia Experimentalis et Applicata, 136(1), 53-65.
http://dx.doi.org/10.1111/j.1570-7458.2010.01002.x
---------- CHICAGO ----------
Lanzavecchia, S., Remis, M., Cladera, J., Zandomeni, R. "Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata" . Entomologia Experimentalis et Applicata 136, no. 1 (2010) : 53-65.
http://dx.doi.org/10.1111/j.1570-7458.2010.01002.x
---------- MLA ----------
Lanzavecchia, S., Remis, M., Cladera, J., Zandomeni, R. "Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata" . Entomologia Experimentalis et Applicata, vol. 136, no. 1, 2010, pp. 53-65.
http://dx.doi.org/10.1111/j.1570-7458.2010.01002.x
---------- VANCOUVER ----------
Lanzavecchia, S., Remis, M., Cladera, J., Zandomeni, R. Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata. Entomol. Exp. Appl. 2010;136(1):53-65.
http://dx.doi.org/10.1111/j.1570-7458.2010.01002.x