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Abstract:

Polycystic ovary syndrome (PCOS) is a frequent pathology that affects more than 5% of women of reproductive age. Among other heterogeneous symptoms, PCOS is characterized by abnormalities in angiogenesis. Metformin has been introduced in the treatment of PCOS to manage insulin resistance and hyperglycemia. Besides its metabolic effects, metformin has been shown to improve ovulation, pregnancy and live birth rates in PCOS patients. In the present study, we used a dehydroepiandrosterone-induced PCOS rat model to analyze the effect of metformin administrationon ovarian angiogenesis.Wefound that metformin was able to restore the increased levels of vascular endothelial growth factor, angiopoietin (ANGPT)1, and ANGPT1/ANGPT2 ratio and the decreased levels of platelet-derived growth factor B and platelet-derived growth factor D observed in the dehydroepiandrosterone-treated rats. These effects could take place, at least in part, through a decrease in the levels of serum insulin. We also found an improvement in follicular development, with a lower percentage of small follicles and cysts and a higher percentage of antral follicles and corpora lutea after metformin administration. The improvement in ovarian angiogenesis is likely to restore the accumulation of small follicles observed in PCOS rats and to reduce cyst formation, thus improving follicular development and the percentage of corpora lutea. These results open new insights into the study of metformin action not only in glucose metabolism but also in ovarian dysfunction in PCOS women. Copyright © 2015 by the Endocrine Society.

Registro:

Documento: Artículo
Título:Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model
Autor:Di Pietro, M.; Parborell, F.; Irusta, G.; Pascuali, N.; Bas, D.; Bianchi, M.S.; Tesone, M.; Abramovich, D.
Filiación:Inst. de Biologia Y Med. Exp.-Consejo Nacional de Investigaciones Cientificas Y Tecnicas (CONICET), Vuelta de Obligado 2490, Buenos Aires, C1428ADN, Argentina
Departamento de Química Biológica Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina
Palabras clave:angiopoietin 1; angiopoietin 2; insulin; metformin; platelet derived growth factor B; platelet derived growth factor D; prasterone; vasculotropin; vasculotropin receptor 2; angiogenesis modulator; angiopoietin 1; angiopoietin 2; metformin; platelet derived growth factor; prasterone; vasculotropin A; angiogenesis; animal experiment; animal model; Article; birth rate; cell structure; controlled study; corpus luteum; female; glucose metabolism; hyperglycemia; insulin blood level; insulin resistance; live birth; nonhuman; ovary follicle cell; ovary polycystic disease; ovulation; pregnancy; priority journal; protein expression; rat; animal; blood; drug effects; metabolism; Neovascularization, Pathologic; ovary follicle; pathophysiology; Polycystic Ovary Syndrome; Sprague Dawley rat; Angiogenesis Modulating Agents; Angiopoietin-1; Angiopoietin-2; Animals; Dehydroepiandrosterone; Female; Insulin; Insulin Resistance; Metformin; Neovascularization, Pathologic; Ovarian Follicle; Platelet-Derived Growth Factor; Polycystic Ovary Syndrome; Rats; Rats, Sprague-Dawley; Vascular Endothelial Growth Factor A
Año:2015
Volumen:156
Número:4
Página de inicio:1453
Página de fin:1463
DOI: http://dx.doi.org/10.1210/en.2014-1765
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:angiopoietin 1, 186270-49-5; angiopoietin 2, 194368-66-6; insulin, 9004-10-8; metformin, 1115-70-4, 657-24-9; prasterone, 53-43-0; vasculotropin, 127464-60-2; vasculotropin A, 489395-96-2; Angiogenesis Modulating Agents; Angiopoietin-1; Angiopoietin-2; Dehydroepiandrosterone; Insulin; Metformin; Platelet-Derived Growth Factor; Vascular Endothelial Growth Factor A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v156_n4_p1453_DiPietro

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Citas:

---------- APA ----------
Di Pietro, M., Parborell, F., Irusta, G., Pascuali, N., Bas, D., Bianchi, M.S., Tesone, M.,..., Abramovich, D. (2015) . Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model. Endocrinology, 156(4), 1453-1463.
http://dx.doi.org/10.1210/en.2014-1765
---------- CHICAGO ----------
Di Pietro, M., Parborell, F., Irusta, G., Pascuali, N., Bas, D., Bianchi, M.S., et al. "Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model" . Endocrinology 156, no. 4 (2015) : 1453-1463.
http://dx.doi.org/10.1210/en.2014-1765
---------- MLA ----------
Di Pietro, M., Parborell, F., Irusta, G., Pascuali, N., Bas, D., Bianchi, M.S., et al. "Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model" . Endocrinology, vol. 156, no. 4, 2015, pp. 1453-1463.
http://dx.doi.org/10.1210/en.2014-1765
---------- VANCOUVER ----------
Di Pietro, M., Parborell, F., Irusta, G., Pascuali, N., Bas, D., Bianchi, M.S., et al. Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model. Endocrinology. 2015;156(4):1453-1463.
http://dx.doi.org/10.1210/en.2014-1765