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Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-β1 is involved, at least in part, in mediating these actions. It was described that TGF-β1 synthesis in rat pituitary lactotrophs is up-regulated by dopamine and down-regulated by estradiol. TGF-β1 is secreted as a large latent complex. The local regulation of cytokine activation in the pituitary has not yet been explored. In this work, we studied pituitary active and total TGF-β1 content, as well as TGF-β1 mRNA, and the in vivo role of dopamine and estradiol on pituitary TGF-β1 levels. Adult female mice (wild type), and female mice with a null mutation in the Drd2 (Drd2-/-), were used. The loss of dopaminergic tone induced a decrease in TGF-β1 mRNA expression, in active and total cytokine content, and in TGF-β type II receptor expression. Dopamine regulation of pituitary TGF-β1 activation process was inferred by the inhibition of active cytokine by in vivo sulpiride treatment. Interestingly, in the absence of dopaminergic tone, estradiol induced a strong increase in active TGF-β1. PRL secretion correlated with active, but not total cytokine. TGF-β1 inhibitory action on lactotroph proliferation and PRL secretion was decreased in Drd2-/- pituitary cells, in correlation with decreased TGF-β type II receptor. The study of the TGF-β1 activation process and its regulation is essential to understand the cytokine activity. As an intermediary of dopamine inhibition of lactotroph function, TGF-β1 and local activators may be important targets in the treatment of dopamine agonist-resistant prolactinomas. Copyright © 2011 by The Endocrine Society.


Documento: Artículo
Título:Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary
Autor:Recouvreux, M.V.; Guida, M.C.; Rifkin, D.B.; Becu-Villalobos, D.; Díaz-Torga, G.
Filiación:Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Department of Cell Biology, New York University Medical Center, New York, NY 10016, United States
Palabras clave:dopamine; dopamine 2 receptor; estradiol; messenger RNA; prolactin; sulpiride; transforming growth factor beta1; transforming growth factor beta2; allele; animal cell; animal experiment; article; cell proliferation; female; hypophysis; hypophysis cell; in vitro study; in vivo study; mouse; nonhuman; priority journal; prolactin secreting cell; Animals; Cell Proliferation; Cells, Cultured; Dopamine Agonists; Dopamine Antagonists; Estradiol; Female; Gene Expression Regulation; Hyperprolactinemia; Lactotrophs; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Transgenic; Pituitary Gland, Anterior; Prolactin; Protein-Serine-Threonine Kinases; Receptors, Dopamine D2; Receptors, Transforming Growth Factor beta; RNA, Messenger; Transforming Growth Factor beta1
Página de inicio:2722
Página de fin:2730
Título revista:Endocrinology
Título revista abreviado:Endocrinology
CAS:dopamine, 51-61-6, 62-31-7; estradiol, 50-28-2; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; sulpiride, 15676-16-1; Dopamine Agonists; Dopamine Antagonists; Estradiol, 50-28-2; Prolactin, 9002-62-4; Protein-Serine-Threonine Kinases,; RNA, Messenger; Receptors, Dopamine D2; Receptors, Transforming Growth Factor beta; Tgfb1 protein, mouse; Transforming Growth Factor beta1; estradiol valerate, 979-32-8; transforming growth factor-beta type II receptor,


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---------- APA ----------
Recouvreux, M.V., Guida, M.C., Rifkin, D.B., Becu-Villalobos, D. & Díaz-Torga, G. (2011) . Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary. Endocrinology, 152(7), 2722-2730.
---------- CHICAGO ----------
Recouvreux, M.V., Guida, M.C., Rifkin, D.B., Becu-Villalobos, D., Díaz-Torga, G. "Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary" . Endocrinology 152, no. 7 (2011) : 2722-2730.
---------- MLA ----------
Recouvreux, M.V., Guida, M.C., Rifkin, D.B., Becu-Villalobos, D., Díaz-Torga, G. "Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary" . Endocrinology, vol. 152, no. 7, 2011, pp. 2722-2730.
---------- VANCOUVER ----------
Recouvreux, M.V., Guida, M.C., Rifkin, D.B., Becu-Villalobos, D., Díaz-Torga, G. Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary. Endocrinology. 2011;152(7):2722-2730.