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Abstract:

Arachidonic acid (AA) is generated in the anterior pituitary gland upon stimulation by the ACTH secretagogue, CRH. Using the patch clamp technique, we examined the action of AA on the excitability of single pituitary corticotropes obtained from a transgenic mouse strain that expresses the enhanced green fluorescent protein driven by the proopiomelanocortin promoter. CRH evoked depolarization, but AA caused hyperpolarization. Under voltage clamp condition, AA caused a rapid inhibition of the delayed rectifier K+ current and then increased a background K+ current. Inhibition of AA metabolism did not prevent the activation of the K+ current by AA, suggesting a direct action of AA. The sensitivity of the AA-activated K+ current to fluoxetine, chlorpromazine, extracellular acidification, diphenylbutylpiperidine antipsychotics, and the membrane permeable cAMP analog [8-(4-chlorophenylthio)-cAMP] suggest that the current is mediated via TWIK-related K+ channel (TREK)-1 channels. Activation of the CRH receptors that are coupled to the adenylate cyclase pathway suppressed the activation of TREK-1 current by AA and reversed the AA-mediated hyperpolarization. Intracellular acidification (pH 7.0) increased the basal amplitude of TREK-1 current and resulted in hyperpolarizaton. CRH suppressed the basal TREK-1 current in cells with intracellular acidification and caused depolarization. Our finding indicates that TREK-1 channels are important in setting the resting potential in corticotropes. The opposing actions of CRH and AA on the excitability of corticotropes raise the possibility that AA may act as a negative feedback regulator to reduce the stimulatory action of CRH and thus prevent excessive ACTH release during chronic stress. Copyright © 2011 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes
Autor:Lee, A.K.; Smart, J.L.; Rubinstein, M.; Low, M.J.; Tse, A.
Filiación:Department of Pharmacology, University of Alberta, Edmonton, AB T6G 2H7, Canada
Department of Molecular and Integrative Physiology, Brehm Diabetes Centre, University of Michigan, Ann Arbor, MI 48105, United States
Department of Biology, George Fox University, Newberg, OR 97132, United States
Instituto de Investigaciones en Ingenieria Genética y Biologia Molecular, Departamento Fisiologia, Biologia Molecular y Celular, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Department of Pharmacology, University of Alberta, 9-70 Medical Sciences Building, Edmonton, AB T6G 2H7, Canada
Palabras clave:8 (4 chlorophenylthio) cyclic AMP; adenylate cyclase; arachidonic acid; chlorpromazine; corticotropin releasing factor; diphenylbutylpiperidine derivative; enhanced green fluorescent protein; fluoxetine; potassium channel; potassium ion; twik related potassium channel 1; unclassified drug; acidification; ACTH secreting cell; animal cell; article; controlled study; fatty acid metabolism; hyperpolarization; membrane depolarization; mouse; nonhuman; potassium current; priority journal; protein expression; voltage clamp; Animals; Arachidonic Acid; Cells, Cultured; Chlorpromazine; Corticotrophs; Corticotropin-Releasing Hormone; Cyclic AMP; Fluoxetine; Green Fluorescent Proteins; Hydrogen-Ion Concentration; Membrane Potentials; Mice; Mice, Transgenic; Neuroprotective Agents; Patch-Clamp Techniques; Potassium Channels, Tandem Pore Domain; Thionucleotides
Año:2011
Volumen:152
Número:5
Página de inicio:1901
Página de fin:1910
DOI: http://dx.doi.org/10.1210/en.2010-1066
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:8 (4 chlorophenylthio) cyclic AMP, 41941-66-6; adenylate cyclase, 9012-42-4; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; chlorpromazine, 50-53-3, 69-09-0; corticotropin releasing factor, 9015-71-8; fluoxetine, 54910-89-3, 56296-78-7, 59333-67-4; potassium ion, 24203-36-9; 8-((4-chlorophenyl)thio)cyclic-3',5'-AMP, 41941-66-6; Arachidonic Acid, 506-32-1; Chlorpromazine, 50-53-3; Corticotropin-Releasing Hormone, 9015-71-8; Cyclic AMP, 60-92-4; Fluoxetine, 54910-89-3; Green Fluorescent Proteins, 147336-22-9; Neuroprotective Agents; Potassium Channels, Tandem Pore Domain; Thionucleotides; potassium channel protein TREK-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v152_n5_p1901_Lee

Referencias:

  • Won, J.G.S., Orth, D.N., Roles of intracellular and extracellular calcium in the kinetic profile of adrenocorticotropin secretion by perifused rat anterior pituitary cells. I. Corticotropin-releasing factor stimulation (1990) Endocrinology, 126 (2), pp. 849-857
  • Lee, A.K., Tse, A., Mechanism underlying corticotropin-releasing hormone (CRH) triggered cytosolic Ca2+ rise in identified rat corticotrophs (1997) Journal of Physiology, 504 (2), pp. 367-378. , DOI 10.1111/j.1469-7793.1997.367be.x
  • Tse, A., Lee, A.K., Voltage-gated Ca2 + channels and intracellular Ca2+ release regulate exocytosis in identified rat corticotrophs (2000) J. Physiol., 528 (1 PART), pp. 79-90
  • Abou-Samra, A.B., Catt, K.J., Aguilera, G., Role arachidonic acid in the regulation of adrenocorticotropin release from rat anterior pituitary cell cultures (1986) Endocrinology, 119 (4), pp. 1427-1431
  • Won, J.G.S., Orth, D.N., Role of lipoxygenase metabolites of arachidonic acid in the regulation of adrenocorticotropin secretion by perifused rat anterior pituitary cells (1994) Endocrinology, 135 (4), pp. 1496-1503. , DOI 10.1210/en.135.4.1496
  • Cowell, A.M., Flower, R.J., Buckingham, J.C., Studies on the roles of phospholipase A2 and eicosanoids in the regulation of corticotrophin secretion by rat pituitary cells in vitro (1991) J. Endocrinol., 130, pp. 21-32
  • Meves, H., Arachidonic acid and ion channels: An update (2008) Br. J. Pharmacol., 155, pp. 4-16
  • Patel, A.J., Honore, E., Properties and modulation of mammalian 2P domain K+ channels (2001) Trends in Neurosciences, 24 (6), pp. 339-346. , DOI 10.1016/S0166-2236(00)01810-5, PII S0166223600018105
  • Cowley, M.A., Smart, J.L., Rubinstein, M., Cerdan, M.G., Diano, S., Horvath, T.L., Cone, R.D., Low, M.J., Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus (2001) Nature, 411 (6836), pp. 480-484. , DOI 10.1038/35078085
  • Ibrahim, N., Bosch, M.A., Smart, J.L., Qiu, J., Rubinstein, M., Ronnekleiv, O.K., Low, M.J., Kelly, M.J., Hypothalamic proopiomelanocortin neurons are glucose responsive and express KATP channels (2003) Endocrinology, 144 (4), pp. 1331-1340. , DOI 10.1210/en.2002-221033
  • Young, J.I., Otero, V., Cerdan, M.G., Falzone, T.L., Cheng Chan, E., Low, M.J., Rubinstein, M., Authentic cell-specific and developmentally regulated expression of pro- opiomelanocortin genomic fragments in hypothalamic and hindbrain neurons of transgenic mice (1998) Journal of Neuroscience, 18 (17), pp. 6631-6640
  • De Souza, F.S.J., Santangelo, A.M., Bumaschny, V., Avale, M.E., Smart, J.L., Low, M.J., Rubinstein, M., Identification of neuronal enhancers of the proopiomelanocortin gene by transgenic mouse analysis and phylogenetic footprinting (2005) Molecular and Cellular Biology, 25 (8), pp. 3076-3086. , DOI 10.1128/MCB.25.8.3076-3086.2005
  • Tse, A., Hille, B., Patch clamping studies on identified pituitary gonadotropes in vitro (1994) Methods Neurosci., 20, pp. 85-99
  • Smith, P.F., Frawley, L.S., Neill, J.D., Detection of LH release from individual pituitary cells by the reverse hemolytic plaque assay: Estrogen increases the fraction of conadotropes responding to GnRH (1984) Endocrinology, 115 (6), pp. 2484-2486
  • Tse, A., Lee, A.K., Arginine vasopressin triggers intracellular calcium release, a calcium- activated potassium current and exocytosis in identified rat corticotropes (1998) Endocrinology, 139 (5), pp. 2246-2252
  • Enyedi, P., Czirják, G., Molecular background of leak K+ currents: Two-pore domain potassium channels (2010) Physiol. Rev., 90, pp. 559-605
  • Tobias, L.D., Hamilton, J.G., The effect of 5,8,11,14-eicosatetraynoic acid on lipid metabolism (1979) Lipids, 14 (2), pp. 181-193
  • Heurteaux, C., Lucas, G., Guy, N., El Yacoubi, M., Thummler, S., Peng, X.-D., Noble, F., Lazdunski, M., Deletion of the background potassium channel TREK-1 results in a depression-resistant phenotype (2006) Nature Neuroscience, 9 (9), pp. 1134-1141. , DOI 10.1038/nn1749, PII NN1749
  • Kennard, L.E., Chumbley, J.R., Ranatunga, K.M., Armstrong, S.J., Veale, E.L., Mathie, A., Inhibition of the human two-pore domain potassium channel, TREK-1, by fluoxetine and its metabolite norfluoxetine (2005) British Journal of Pharmacology, 144 (6), pp. 821-829. , DOI 10.1038/sj.bjp.0706068
  • Thummler, S., Duprat, F., Lazdunski, M., Antipsychotics inhibit TREK but not TRAAK channels (2007) Biochemical and Biophysical Research Communications, 354 (1), pp. 284-289. , DOI 10.1016/j.bbrc.2006.12.199, PII S0006291X06028907
  • Czirjak, G., Enyedi, P., Formation of functional heterodimers between the TASK-1 and TASK-3 two-pore domain potassium channel subunits (2002) Journal of Biological Chemistry, 277 (7), pp. 5426-5432. , DOI 10.1074/jbc.M107138200
  • Sandoz, G., Douguet, D., Chatelain, F., Lazdunski, M., Lesage, F., Extracellular acidification exerts opposite actions on TREK1 and TREK2 potassium channels via a single conserved histidine residue (2009) Proc. Natl. Acad. Sci. USA, 106, pp. 14628-14633
  • Gomora, J.C., Enyeart, J.J., Dual pharmacological properties of a cyclic AMP-sensitive potassium channel (1999) Journal of Pharmacology and Experimental Therapeutics, 290 (1), pp. 266-275
  • Liu, H., Enyeart, J.A., Enyeart, J.J., Ohio, Potent inhibition of native TREK-1 K+ channels by selected dihydropyridine Ca2+ channel antagonists (2007) Journal of Pharmacology and Experimental Therapeutics, 323 (1), pp. 39-48. , http://jpet.aspetjournals.org/cgi/reprint/323/1/39, DOI 10.1124/jpet.107.125245
  • Smirnov, S.V., Aaronson, P.I., Modulatory effects of arachidonic acid on the delayed rectifier K + current in rat pulmonary arterial myocytes: Structural aspects and involvement of protein kinase C (1996) Circulation Research, 79 (1), pp. 20-31
  • Danthi, S., Enyeart, J.A., Enyeart, J.J., Modulation of Native TREK-1 and Kv1.4 K+ Channels by Polyunsaturated Fatty Acids and Lysophospholipids (2003) Journal of Membrane Biology, 195 (3), pp. 147-164. , DOI 10.1007/s00232-003-0616-0
  • Honore, E., The neuronal background K2P channels: Focus on TREK1 (2007) Nature Reviews Neuroscience, 8 (4), pp. 251-261. , DOI 10.1038/nrn2117, PII NRN2117
  • Liu, H., Enyeart, J.A., Enyeart, J.J., ACTH inhibits bTREK-1 K+ channels through multiple cAMP-dependent signaling pathways (2008) J. Gen. Physiol., 132, pp. 279-294
  • Maingret, F., Honore, E., Lazdunski, M., Patel, A.J., Molecular basis of the voltage-dependent gating of TREK-1, a mechano-sensitive K+ channel (2002) Biochemical and Biophysical Research Communications, 292 (2), pp. 339-346. , DOI 10.1006/bbrc.2002.6674
  • Yeung, S.Y., Millar, J.A., Mathie, A., Inhibition of neuronal KV potassium currents by the antidepressant drug, fluoxetine (1999) Br. J. Pharmacol., 128, pp. 1609-1615

Citas:

---------- APA ----------
Lee, A.K., Smart, J.L., Rubinstein, M., Low, M.J. & Tse, A. (2011) . Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes. Endocrinology, 152(5), 1901-1910.
http://dx.doi.org/10.1210/en.2010-1066
---------- CHICAGO ----------
Lee, A.K., Smart, J.L., Rubinstein, M., Low, M.J., Tse, A. "Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes" . Endocrinology 152, no. 5 (2011) : 1901-1910.
http://dx.doi.org/10.1210/en.2010-1066
---------- MLA ----------
Lee, A.K., Smart, J.L., Rubinstein, M., Low, M.J., Tse, A. "Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes" . Endocrinology, vol. 152, no. 5, 2011, pp. 1901-1910.
http://dx.doi.org/10.1210/en.2010-1066
---------- VANCOUVER ----------
Lee, A.K., Smart, J.L., Rubinstein, M., Low, M.J., Tse, A. Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes. Endocrinology. 2011;152(5):1901-1910.
http://dx.doi.org/10.1210/en.2010-1066