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Abstract:

We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes. Copyright © 2006 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
Autor:Frungieri, M.B.; Gonzalez-Calvar, S.I.; Parborell, F.; Albrecht, M.; Mayerhofer, A.; Calandra, R.S.
Filiación:Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas, 1428 Buenos Aires, Argentina
Anatomical Institute, Ludwig Maximilians University, D-80802 Munich, Germany
Facultad de Medicina, Universidad de Buenos Aires, 1121 Buenos Aires, Argentina
Instituto Multidisciplinario de Biología Celular, 1900 La Plata, Argentina
Instituto de Biología Y Medicina Experimental, CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:chorionic gonadotropin; cyclooxygenase 2; luteinizing hormone; meloxicam; prostaglandin; prostaglandin F2 alpha; steroidogenic acute regulatory protein; testosterone; testosterone 17beta dehydrogenase; animal cell; animal experiment; article; controlled study; electron microscopy; enzyme inhibition; experimental model; gene expression; hamster; hormone synthesis; human; human cell; immunohistochemistry; Leydig cell; male infertility; nonhuman; nucleotide sequence; priority journal; protein expression; reverse transcription polymerase chain reaction; Sertoli cell; testis; testis biopsy; testosterone synthesis; 17-Hydroxysteroid Dehydrogenases; Adult; Animals; Chorionic Gonadotropin; Cricetinae; Cyclooxygenase 1; Cyclooxygenase 2; Dinoprost; Gene Expression; Humans; Immunohistochemistry; Leydig Cells; Luteinizing Hormone; Male; Mesocricetus; Phosphoproteins; Receptors, Prostaglandin; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Testis; Testosterone
Año:2006
Volumen:147
Número:9
Página de inicio:4476
Página de fin:4485
DOI: http://dx.doi.org/10.1210/en.2006-0090
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:chorionic gonadotropin, 9002-61-3; luteinizing hormone, 39341-83-8, 9002-67-9; meloxicam, 71125-38-7; prostaglandin F2 alpha, 551-11-1; steroidogenic acute regulatory protein, 168183-61-7; testosterone 17beta dehydrogenase, 9028-62-0; testosterone, 58-22-0; 17-Hydroxysteroid Dehydrogenases, EC 1.1.-; Chorionic Gonadotropin; Cyclooxygenase 1, EC 1.14.99.1; Cyclooxygenase 2, EC 1.14.99.1; Dinoprost, 551-11-1; Luteinizing Hormone, 9002-67-9; Phosphoproteins; prostaglandin F2alpha receptor; Receptors, Prostaglandin; RNA, Messenger; steroidogenic acute regulatory protein; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v147_n9_p4476_Frungieri

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Citas:

---------- APA ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Parborell, F., Albrecht, M., Mayerhofer, A. & Calandra, R.S. (2006) . Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production. Endocrinology, 147(9), 4476-4485.
http://dx.doi.org/10.1210/en.2006-0090
---------- CHICAGO ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Parborell, F., Albrecht, M., Mayerhofer, A., Calandra, R.S. "Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production" . Endocrinology 147, no. 9 (2006) : 4476-4485.
http://dx.doi.org/10.1210/en.2006-0090
---------- MLA ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Parborell, F., Albrecht, M., Mayerhofer, A., Calandra, R.S. "Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production" . Endocrinology, vol. 147, no. 9, 2006, pp. 4476-4485.
http://dx.doi.org/10.1210/en.2006-0090
---------- VANCOUVER ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Parborell, F., Albrecht, M., Mayerhofer, A., Calandra, R.S. Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production. Endocrinology. 2006;147(9):4476-4485.
http://dx.doi.org/10.1210/en.2006-0090