Artículo

Labombarda, F.; Pianos, A.; Liere, P.; Eychenne, B.; Gonzalez, S.; Cambourg, A.; De Nicola, A.F.; Schumacher, M.; Guennoun, R. "Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry" (2006) Endocrinology. 147(4):1847-1859
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Abstract:

The effects of spinal cord injury (SCI), combined with castration and adrenalectomy, and of progesterone (PROG) treatment on neurosteroid levels and steroidogenic enzyme expression were investigated in the adult male rat spinal cord (SC). Steroid levels were quantified by gas chromatography/mass spectrometry in SC and plasma, and mRNAs of enzymes by quantitative real-time RT-PCR. The levels of pregnenolone (PREG), PROG, 5α-dihydroprogesterone, 3α,5α-tetrahydroprogesterone increased in SC 75 h after transection without significant increase in the plasma. After combined adrenalectomy and gonadectomy, significant levels of PREG and PROG remained in the SC, suggesting their local biosynthesis. In the SC of adrenalectomized and gonadectomized rats, there was an increase of PREG 24 h after SCI, followed at 75 h by a concomitant increase in its direct metabolite, PROG. These observations are consistent with a sequential increase of PREG biosynthesis and its conversion to PROG within the SC in response to injury. However, no significant change in P450-side chain cleavage and 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase mRNA levels was observed after SCI. Systemic PROG treatment after SCI, resulted in a very large increase in PROG, 5α-dihydroprogesterone, and 3α,5α-tetrahydroprogesterone in both plasma and SC. Furthermore, high levels of 3β,5α-tetrahydroprogesterone were detected in SC, whereas their plasma levels remained barely detectable. Because the ratio of reduced metabolites to PROG was 65-times higher in SC than in the plasma, it appears likely that reduced metabolites mainly originated from local biosynthesis. Our results strongly suggest an important role for locally biosynthesized neurosteroids in the response of the SC to injury. Copyright © 2006 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry
Autor:Labombarda, F.; Pianos, A.; Liere, P.; Eychenne, B.; Gonzalez, S.; Cambourg, A.; De Nicola, A.F.; Schumacher, M.; Guennoun, R.
Filiación:Institut National de la Santé et de la Recherche Médicale Unité 488, Stéroïdes et Système Nerveux, University Paris 11, 94276 Kremlin-Bicêtre, France
Instituto de Biologia Y Medicina Experimental, Department of Human Biochemistry, University of Buenos Aires, 1428 Buenos Aires, Argentina
Institut National de la Santé et de la Recherche Médicale Unité 488, 80 R. du Gén. Leclerc, 94276 Bicêtre, France
Palabras clave:3(or 17)beta hydroxysteroid dehydrogenase; 3alpha hydroxy 5alpha pregnan 20 one; 5alpha pregnane 3,20 dione; cytochrome P450; messenger RNA; neurosteroid; pregnenolone; progesterone; steroid delta isomerase; adrenalectomy; animal experiment; article; biosynthesis; controlled study; gas chromatography; gonadectomy; male; mass spectrometry; nonhuman; priority journal; quantitative analysis; rat; reverse transcription polymerase chain reaction; spinal cord; 17-Hydroxysteroid Dehydrogenases; 5-alpha-Dihydroprogesterone; Animals; Cholesterol Side-Chain Cleavage Enzyme; Gas Chromatography-Mass Spectrometry; Male; Pregnanolone; Pregnenolone; Progesterone; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Spinal Cord; Spinal Cord Injuries
Año:2006
Volumen:147
Número:4
Página de inicio:1847
Página de fin:1859
DOI: http://dx.doi.org/10.1210/en.2005-0955
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; 3alpha hydroxy 5alpha pregnan 20 one, 516-54-1; 5alpha pregnane 3,20 dione, 566-65-4; cytochrome P450, 9035-51-2; pregnenolone, 145-13-1; progesterone, 57-83-0; steroid delta isomerase, 9031-36-1; 17-Hydroxysteroid Dehydrogenases, EC 1.1.-; 3 (or 17)-beta-hydroxysteroid dehydrogenase, EC 1.1.1.51; 5-alpha-Dihydroprogesterone, 566-65-4; Cholesterol Side-Chain Cleavage Enzyme, EC 1.14.15.6; Pregnanolone, 128-20-1; Pregnenolone, 145-13-1; Progesterone, 57-83-0; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v147_n4_p1847_Labombarda

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Citas:

---------- APA ----------
Labombarda, F., Pianos, A., Liere, P., Eychenne, B., Gonzalez, S., Cambourg, A., De Nicola, A.F.,..., Guennoun, R. (2006) . Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry. Endocrinology, 147(4), 1847-1859.
http://dx.doi.org/10.1210/en.2005-0955
---------- CHICAGO ----------
Labombarda, F., Pianos, A., Liere, P., Eychenne, B., Gonzalez, S., Cambourg, A., et al. "Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry" . Endocrinology 147, no. 4 (2006) : 1847-1859.
http://dx.doi.org/10.1210/en.2005-0955
---------- MLA ----------
Labombarda, F., Pianos, A., Liere, P., Eychenne, B., Gonzalez, S., Cambourg, A., et al. "Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry" . Endocrinology, vol. 147, no. 4, 2006, pp. 1847-1859.
http://dx.doi.org/10.1210/en.2005-0955
---------- VANCOUVER ----------
Labombarda, F., Pianos, A., Liere, P., Eychenne, B., Gonzalez, S., Cambourg, A., et al. Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry. Endocrinology. 2006;147(4):1847-1859.
http://dx.doi.org/10.1210/en.2005-0955