Artículo

Cristina, C.; Díaz-Torga, G.; Baldi, A.; Góngora, A.; Rubinstein, M.; Low, M.J.; Becú-Villalobos, D. "Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice" (2005) Endocrinology. 146(7):2952-2962
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Abstract:

Vascular endothelial growth factor (VEGF)-A is an important angiogenic cytokine in cancer and pathological angiogenesis and has been related to the antiangiogenic activity of dopamine in endothelial cells. We investigated VEGF expression, localization, and function in pituitary hyperplasia of dopamine D2 receptor (D2R)-knockout female mice. Pituitaries from knockout mice showed increased protein and mRNA VEGF-A expression when compared with wild-type mice. In wild-type mice, prolonged treatment with the D2R antagonist, haloperidol, enhanced pituitary VEGF expression and prolactin release, suggesting that dopamine inhibits pituitary VEGF expression. VEGF expression was also increased in pituitary cells from knockout mice, even though these cells proliferated less in vitro when compared with wild-type cells, as determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium proliferation assay, proliferating cell nuclear antigen expression, and [3H]thymidine incorporation. In contrast to other animal models, estrogen did not increase pituitary VEGF protein and mRNA expression and lowered serum prolactin secretion in vivo and in vitro in both genotypes. VEGF(10 and 30 ng/ml) did not modify pituitary cell proliferation in either genotype and increased prolactin secretion in vitro in estrogen-pretreated cells of both genotypes. But conditioned media from D2R -/- cells enhanced human umbilical vein cell proliferation, and this effect could be partially inhibited by an anti-VEGF antiserum. Finally, using dual-labeling immunofluorescence and confocal laser microscopy, we found that in the hyperplastic pituitaries, VEGF-A was mostly present in follicle-stellate cells. In conclusion, pituitary VEGF expression is under dopaminergic control, and even though VEGF does not promote pituitary cellular proliferation in vitro, it may be critical for pituitary angiogenesis through paracrine actions in the D2R knockout female mice. Copyright © 2005 by The Endocrine Society.

Registro:

Documento: Artículo
Título:Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice
Autor:Cristina, C.; Díaz-Torga, G.; Baldi, A.; Góngora, A.; Rubinstein, M.; Low, M.J.; Becú-Villalobos, D.
Filiación:Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Argentina
Instituto de Biología Y Medicina Experimental, 1428 Buenos Aires, Argentina
Instituto de Investigaciones en Ingeniería Genética Y Biología Molecular, 1428 Buenos Aires, Argentina
University of Buenos Aires, 1428 Buenos Aires, Argentina
Vollum Institute, Oregon Health and Science University, Portland, OR 97239-3098, United States
Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas, V. Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:3 (4,5 dimethylthiazol 2 yl) 5 (3 carboxymethoxyphenyl) 2 (4 sulfophenyl) 2h tetrazolium; cell nucleus antigen; dopamine 2 receptor; dopamine 2 receptor blocking agent; estrogen; haloperidol; messenger RNA; prolactin; tetrazolium; thymidine; tritium; unclassified drug; vasculotropin A; dopamine 2 receptor; dopamine receptor blocking agent; drug derivative; estrogen; haloperidol; haloperidol decanoate; prolactin; vasculotropin A; angiogenesis; animal cell; animal experiment; animal model; animal tissue; antigen expression; article; cell proliferation; comparative study; confocal laser microscopy; controlled study; correlation analysis; female; genotype; hyperplasia; hypophysis cell; immunofluorescence test; in vitro study; in vivo study; knockout mouse; mouse; nonhuman; paracrine signaling; priority journal; prolactin release; protein expression; protein localization; stellate cell; umbilical vein; wild type; animal; blood; cell culture; chemistry; culture medium; cytology; drug effect; genetics; human; hypophysis; metabolism; mouse mutant; pathology; tissue distribution; Animals; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; Dopamine Antagonists; Estrogens; Female; Haloperidol; Humans; Hyperplasia; Mice; Mice, Knockout; Pituitary Gland; Prolactin; Receptors, Dopamine D2; RNA, Messenger; Tissue Distribution; Umbilical Veins; Vascular Endothelial Growth Factor A
Año:2005
Volumen:146
Número:7
Página de inicio:2952
Página de fin:2962
DOI: http://dx.doi.org/10.1210/en.2004-1445
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:haloperidol, 52-86-8; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; thymidine, 50-89-5; tritium, 10028-17-8; vasculotropin A, 489395-96-2; haloperidol decanoate, 74050-97-8; Culture Media, Conditioned; Dopamine Antagonists; Estrogens; haloperidol decanoate, 74050-97-8; Haloperidol, 52-86-8; Prolactin, 9002-62-4; Receptors, Dopamine D2; RNA, Messenger; Vascular Endothelial Growth Factor A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v146_n7_p2952_Cristina

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Citas:

---------- APA ----------
Cristina, C., Díaz-Torga, G., Baldi, A., Góngora, A., Rubinstein, M., Low, M.J. & Becú-Villalobos, D. (2005) . Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice. Endocrinology, 146(7), 2952-2962.
http://dx.doi.org/10.1210/en.2004-1445
---------- CHICAGO ----------
Cristina, C., Díaz-Torga, G., Baldi, A., Góngora, A., Rubinstein, M., Low, M.J., et al. "Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice" . Endocrinology 146, no. 7 (2005) : 2952-2962.
http://dx.doi.org/10.1210/en.2004-1445
---------- MLA ----------
Cristina, C., Díaz-Torga, G., Baldi, A., Góngora, A., Rubinstein, M., Low, M.J., et al. "Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice" . Endocrinology, vol. 146, no. 7, 2005, pp. 2952-2962.
http://dx.doi.org/10.1210/en.2004-1445
---------- VANCOUVER ----------
Cristina, C., Díaz-Torga, G., Baldi, A., Góngora, A., Rubinstein, M., Low, M.J., et al. Increased pituitary vascular endothelial growth factor-A in dopaminergic D2 receptor knockout female mice. Endocrinology. 2005;146(7):2952-2962.
http://dx.doi.org/10.1210/en.2004-1445