Artículo

Chamson-Reig, A.; Sorianello, E.M.; Catalano, P.N.; Fernández, M.O.; Pignataro, O.P.; Libertun, C.; Lux-Lantos, V.A.R. "Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor" (2003) Endocrinology. 144(7):2957-2966
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Abstract:

Previous results showed that GnRH signaling is altered in cells from rat luteinized ovarian tumors (tumor group) because it did not activate the phospholipase C pathway, in contrast to control ovarian cells from superovulated prepubertal rats (SPO). In the present work, alternate GnRH-induced second messengers such as phospholipase A2 and phospholipase D activation, cAMP production, ERK1/2 phosphorylation, and the presence of G proteins were evaluated to determine GnRH mechanism of action in tumor cells. G proteins examined were present in both cell types. Buserelin, a GnRH agonist, (1, 10, and 100 ng/ml) increased phosphatidylethanol in SPO, indicating phospholipase D activation. Only 100 ng/ml buserelin induced a significant response in the tumor group. Buserelin (100 ng/ml) increased 3H-arachidonic acid in culture media in SPO, indicating phospholipase A2 activation; no effect was observed in the tumor group. Buserelin (100 and 1000 ng/ml) induced pertussis toxin-insensitive cAMP increases in both cell types, with similar potencies. In the tumor group, buserelin (100 ng/ml) inhibited human chorionic gonadotropin-induced cAMP and progesterone; this effect was protein kinase C (PKC) dependent (inhibited by GF109203X, a PKC inhibitor). Buserelin (100 and 1000 ng/ml) induced ERK1/2 phosphorylation in both cell kinds. Buserelin-induced ERK1/2 activation was Gi/0 independent and PKC dependent. Only in the tumor group, buserelin-induced ERK1/2 activation was cAMP dependent (abolished by SQ 22536, the adenylyl cyclase inhibitor). Furthermore, dibutyryl cAMP-induced ERK1/2 activation in the tumor group was PKC dependent (inhibited by GF109203X). In conclusion, activation of phospholipases in tumor cells does not seem to mediate GnRH effects. GnRH signaling seems to involve adenylyl cyclase activation, PKC stimulation, and ERK1/2 phosphorylation.

Registro:

Documento: Artículo
Título:Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor
Autor:Chamson-Reig, A.; Sorianello, E.M.; Catalano, P.N.; Fernández, M.O.; Pignataro, O.P.; Libertun, C.; Lux-Lantos, V.A.R.
Filiación:Inst. Biol. Med. Exp.-Consejo N., Facultad de Medicina, Universidad de Buenos Aires, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
V. de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:2 [1 (3 dimethylaminopropyl) 3 indolyl] 3 (3 indolyl)maleimide; 9 (tetrahydro 2 furyl)adenine; arachidonic acid; buserelin; cyclic AMP; gonadorelin; gonadorelin agonist; guanine nucleotide binding protein; mitogen activated protein kinase; mitogen activated protein kinase 1; phospholipase A2; phospholipase C; phospholipase D; progesterone; animal cell; animal experiment; animal model; article; cell type; controlled study; enzyme activation; female; nonhuman; ovary tumor; prepuberty; priority journal; protein phosphorylation; rat; superovulation; Adenylate Cyclase; Animals; Antineoplastic Agents, Hormonal; Buserelin; Carcinogens; Cyclic AMP; Enzyme Activation; Female; Gonadotropin-Releasing Hormone; GTP-Binding Protein alpha Subunits; GTP-Binding Protein alpha Subunits, Gi-Go; Heterotrimeric GTP-Binding Proteins; Luteoma; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Ovarian Neoplasms; Pertussis Toxin; Phospholipase D; Phospholipases A; Phosphorylation; Progesterone; Protein Kinase C; Rats; Rats, Sprague-Dawley; Signal Transduction; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured
Año:2003
Volumen:144
Número:7
Página de inicio:2957
Página de fin:2966
DOI: http://dx.doi.org/10.1210/en.2003-0011
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:2 [1 (3 dimethylaminopropyl) 3 indolyl] 3 (3 indolyl)maleimide, 133052-90-1; 9 (tetrahydro 2 furyl)adenine, 17318-31-9; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; buserelin, 57982-77-1; cyclic AMP, 60-92-4; gonadorelin, 33515-09-2, 9034-40-6; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase, 142243-02-5; phospholipase A2, 9001-84-7; phospholipase C, 9001-86-9; phospholipase D, 9001-87-0; progesterone, 57-83-0; Adenylate Cyclase, EC 4.6.1.1; Antineoplastic Agents, Hormonal; Buserelin, 57982-77-1; Carcinogens; Cyclic AMP, 60-92-4; Gonadotropin-Releasing Hormone, 33515-09-2; GTP-Binding Protein alpha Subunits; GTP-Binding Protein alpha Subunits, Gi-Go, EC 3.6.1.46; Heterotrimeric GTP-Binding Proteins, EC 3.6.1.46; Mitogen-Activated Protein Kinase 1, EC 2.7.1.37; Mitogen-Activated Protein Kinase 3, EC 2.7.1.37; Mitogen-Activated Protein Kinases, EC 2.7.1.37; olfactory G protein subunit alpha olf; Pertussis Toxin, EC 2.4.2.31; Phospholipase D, EC 3.1.4.4; Phospholipases A, EC 3.1.1.-; Progesterone, 57-83-0; Protein Kinase C, EC 2.7.1.37; Tetradecanoylphorbol Acetate, 16561-29-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v144_n7_p2957_ChamsonReig

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Citas:

---------- APA ----------
Chamson-Reig, A., Sorianello, E.M., Catalano, P.N., Fernández, M.O., Pignataro, O.P., Libertun, C. & Lux-Lantos, V.A.R. (2003) . Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor. Endocrinology, 144(7), 2957-2966.
http://dx.doi.org/10.1210/en.2003-0011
---------- CHICAGO ----------
Chamson-Reig, A., Sorianello, E.M., Catalano, P.N., Fernández, M.O., Pignataro, O.P., Libertun, C., et al. "Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor" . Endocrinology 144, no. 7 (2003) : 2957-2966.
http://dx.doi.org/10.1210/en.2003-0011
---------- MLA ----------
Chamson-Reig, A., Sorianello, E.M., Catalano, P.N., Fernández, M.O., Pignataro, O.P., Libertun, C., et al. "Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor" . Endocrinology, vol. 144, no. 7, 2003, pp. 2957-2966.
http://dx.doi.org/10.1210/en.2003-0011
---------- VANCOUVER ----------
Chamson-Reig, A., Sorianello, E.M., Catalano, P.N., Fernández, M.O., Pignataro, O.P., Libertun, C., et al. Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor. Endocrinology. 2003;144(7):2957-2966.
http://dx.doi.org/10.1210/en.2003-0011