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Two of the most potent cytokines that regulate anterior pituitary cell function are leukemia inhibitory factor and IL-6. These and others like IL-11 and ciliary neurotrophic factor are referred to as the gp130 cytokines because they share the gp130 glycoprotein as a common receptor initial signal transducer. We and others have shown that gp130 cytokines and their receptors are expressed and functional in normal and tumoral anterior pituitary cells. To study the role of gp130 cytokines in tumorigenic process, we generated gp130 cDNA gp130 sense and gp130 antisense (gp130-AS) transfected stable clones derived from lactosomatotroph GH3 cells. We examined hormone secretion and cell proliferation of these clones as well as their tumorigenic properties in athymic nude mice. Although gp130-AS clones, which have low gp130 levels and impaired signal transducer and activator of transcription 3 activity and suppressor of cytokine signaling-3 expression, showed reduced proliferation and hormone secretion (GH and prolactin) in response to gp130 cytokines, they had a normal response to gp130-independent stimuli. Moreover, gp130-AS clones showed a severely impaired in vivo tumor development. In contrast, the overexpressing gp130 clones (gp130 sense) showed no differences, compared with cells transfected with control vector. Thus, the present study provides new evidence supporting a link between gp130 and pituitary abnormal growth.


Documento: Artículo
Título:Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
Autor:Perez Castro, C.; Giacomini, D.; Carbia Nagashima, A.; Onofri, C.; Graciarena, M.; Kobayashi, K.; Páez-Pereda, M.; Renner, U.; Stalla, G.K.; Arzt, E.
Filiación:Depto. Fisiol. y Biol. Molec. y Cel., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
Department of Endocrinology, Max Planck Institute of Psychiatry, 80804 Munich, Germany
Depto. Fisiol. y Biol. Molec. y Cel., Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina
Palabras clave:antisense oligodeoxynucleotide; complementary DNA; cytokine; glycoprotein gp 130; interleukin 11; interleukin 6; leukemia inhibitory factor; adenohypophysis; animal cell; article; carcinogenesis; cell function; cell growth; cell proliferation; cell strain GH3; clone; controlled study; gene expression; gene overexpression; genetic transfection; growth hormone release; hormone release; hypophysis cell; nonhuman; nude mouse; priority journal; prolactin release; prolactin synthesis; rat; signal transduction; transcription initiation; Animals; Antigens, CD; Cell Division; Cytokine Receptor gp130; Cytokines; Gene Expression Regulation, Neoplastic; Hormones; Membrane Glycoproteins; Mice; Mice, Nude; Neoplasm Transplantation; Pituitary Neoplasms; Rats; RNA, Messenger; Tumor Cells, Cultured
Página de inicio:693
Página de fin:700
Título revista:Endocrinology
Título revista abreviado:Endocrinology
CAS:Antigens, CD; Cytokine Receptor gp130, 133483-10-0; Cytokines; Hormones; Il6st protein, mouse; Il6st protein, rat; Membrane Glycoproteins; RNA, Messenger


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---------- APA ----------
Perez Castro, C., Giacomini, D., Carbia Nagashima, A., Onofri, C., Graciarena, M., Kobayashi, K., Páez-Pereda, M.,..., Arzt, E. (2003) . Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells. Endocrinology, 144(2), 693-700.
---------- CHICAGO ----------
Perez Castro, C., Giacomini, D., Carbia Nagashima, A., Onofri, C., Graciarena, M., Kobayashi, K., et al. "Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells" . Endocrinology 144, no. 2 (2003) : 693-700.
---------- MLA ----------
Perez Castro, C., Giacomini, D., Carbia Nagashima, A., Onofri, C., Graciarena, M., Kobayashi, K., et al. "Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells" . Endocrinology, vol. 144, no. 2, 2003, pp. 693-700.
---------- VANCOUVER ----------
Perez Castro, C., Giacomini, D., Carbia Nagashima, A., Onofri, C., Graciarena, M., Kobayashi, K., et al. Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells. Endocrinology. 2003;144(2):693-700.