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Ensinck, J.W.; Baskin, D.G.; Vahl, T.P.; Vogel, R.E.; Laschansky, E.C.; Francis, B.H.; Hoffman, R.C.; Krakover, J.D.; Stamm, M.R.; Low, M.J.; Rubinstein, M.; Otero-Corchon, V.; D'Alessio, D.A. "Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation" (2002) Endocrinology. 143(7):2599-2609
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Abstract:

Preprosomatostatin is a gene expressed ubiquitously among vertebrates, and at least two duplications of this gene have occurred during evolution. Somatostatin-28 (S-28) and somatostatin-14 (S-14), C-terminal products of prosomatostatin (ProS), are differentially expressed in mammalian neurons, D cells, and enterocytes. One pathway for the generation of S-14 entails the excision of Arg13-Lys14 in S-28, leading to equivalent amounts of S-28(1-12). Using an antiserum (F-4), directed to the N-terminal region of S-28 that does not react with S-28(1-12), we detected a peptide, in addition to S-28 and ProS, that was present in human plasma and in the intestinal tract of rats and monkeys. This F-4 reacting peptide was purified from monkey ileum; and its amino acid sequence, molecular mass, and chromatographic characteristics conformed to those of S-28(1-13), a peptide not described heretofore. When extracts of the small intestine were measured by RIA, there was a discordance in the ratio of peptides reacting with F-4 and those containing the C terminus of ProS, suggesting sites of synthesis for S-28(1-13) distinct from those for S-14 and S-28. This was supported by immunocytochemistry, wherein F-4 reactivity was localized in gastrointestinal (GI) endocrine cells and a widespread plexus of neurons within the wall of the distal gut while immunoreactivity to C-terminal domains of S-14 and S-28 in these neurons was absent. Further, F-4 immunoreactivity persisted in similar GI endocrine cells and myenteric neurons in mice with a targeted deletion of the preprosomatostatin gene. We believe that these data suggest a novel peptide produced in the mammalian gut, homologous with the 13 residues of the proximal region of S-28 but not derived from the Pros gene. Pending characterization of the gene from which this peptide is derived, its distribution, and function, we have designated this peptide as thrittene. Its localization in both GI endocrine cells and gut neurons suggests that thrittene may function as both a hormone and neurotransmitter.

Registro:

Documento: Artículo
Título:Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation
Autor:Ensinck, J.W.; Baskin, D.G.; Vahl, T.P.; Vogel, R.E.; Laschansky, E.C.; Francis, B.H.; Hoffman, R.C.; Krakover, J.D.; Stamm, M.R.; Low, M.J.; Rubinstein, M.; Otero-Corchon, V.; D'Alessio, D.A.
Filiación:Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington, Seattle, WA 98195, United States
Department of Biological Structure, University of Washington, Seattle, WA 98195, United States
Department of Veterans Affairs Puget Sound Health Care System, Seattle, WA 98108, United States
ZymoGenetics, Seattle, WA 98102, United States
Vollum Institute, Oregon Health Sciences University, Portland, OR 97201-3098, United States
Division of Endocrinology, University of Cincinnati, OH 45267-0547, United States
University of Cincinnati, Division of Endocrinology, ML 0547, Cincinnati, OH 45267-0547, United States
Pharmaceutical Research Institute, P.O. Box 300, Raritan, NJ 08869-0602, United States
Howard Hughes Medical Institute, University of California at San Diego, Mailstop 0314, 9500 Gilman Drive, San Diego, CA 92103-0314, United States
Argonex, Inc., 706 Forest Street, Charlottesville, VA, United States
Institute de Investigaciones en Ingeneria Genetica y Biologia Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, 1428 Buenos Aires, Argentina
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:arginine; lysine; somatostatin 28; thrittene; unclassified drug; adult; amino acid sequence; article; carboxy terminal sequence; clinical article; gene deletion; gene duplication; gene targeting; human; immunocytochemistry; intestine blood flow; intestine cell; male; multigene family; neurotransmitter release; peptide synthesis; priority journal; protein domain
Año:2002
Volumen:143
Número:7
Página de inicio:2599
Página de fin:2609
DOI: http://dx.doi.org/10.1210/endo.143.7.8904
Título revista:Endocrinology
Título revista abreviado:Endocrinology
ISSN:00137227
CODEN:ENDOA
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; lysine, 56-87-1, 6899-06-5, 70-54-2; somatostatin 28, 75037-27-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v143_n7_p2599_Ensinck

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Citas:

---------- APA ----------
Ensinck, J.W., Baskin, D.G., Vahl, T.P., Vogel, R.E., Laschansky, E.C., Francis, B.H., Hoffman, R.C.,..., D'Alessio, D.A. (2002) . Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation. Endocrinology, 143(7), 2599-2609.
http://dx.doi.org/10.1210/endo.143.7.8904
---------- CHICAGO ----------
Ensinck, J.W., Baskin, D.G., Vahl, T.P., Vogel, R.E., Laschansky, E.C., Francis, B.H., et al. "Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation" . Endocrinology 143, no. 7 (2002) : 2599-2609.
http://dx.doi.org/10.1210/endo.143.7.8904
---------- MLA ----------
Ensinck, J.W., Baskin, D.G., Vahl, T.P., Vogel, R.E., Laschansky, E.C., Francis, B.H., et al. "Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation" . Endocrinology, vol. 143, no. 7, 2002, pp. 2599-2609.
http://dx.doi.org/10.1210/endo.143.7.8904
---------- VANCOUVER ----------
Ensinck, J.W., Baskin, D.G., Vahl, T.P., Vogel, R.E., Laschansky, E.C., Francis, B.H., et al. Thrittene, homologous with somatostatin-28(1-13), is a novel peptide in mammalian gut and circulation. Endocrinology. 2002;143(7):2599-2609.
http://dx.doi.org/10.1210/endo.143.7.8904